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Antibacterial activity of rhodomyrtone on Clostridium difficile vegetative cells and spores in vitro.
Int J Antimicrob Agents. 2018 Nov; 52(5):724-729.IJ

Abstract

The increasing incidence and severity of diarrhoea and colitis caused by Clostridium difficile, together with a high rate of relapse following treatment with currently recommended antimicrobials, calls for novel interventions for C. difficile infection (CDI). Rhodomyrtone, a bioactive compound derived from the leaves of the rose myrtle (Rhodomyrtus tomentosa) has demonstrated antibacterial activity against several Gram-positive bacteria. This study compared the in vitro antimicrobial activity of rhodomyrtone on C. difficile with that of vancomycin, a recommended agent for the treatment of CDI. Determination of the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of rhodomyrtone and vancomycin for ten C. difficile isolates showed that the MICs of rhodomyrtone for C. difficile vegetative cells (0.625-2.5 mg/L) were comparable with that of vancomycin (1.25 mg/L), but the MBCs of rhodomyrtone (1.25-5 mg/L) were significantly lower than those for vancomycin (5 mg/L to ˃40 mg/L; P < 0.001). Time-kill assays showed rapid bactericidal activity for rhodomyrtone, with ≥99% killing within 4 h. Rhodomyrtone was also four-fold more potent than vancomycin in inhibiting C. difficile spore outgrowth. Transmission electron microscopy of rhodomyrtone-treated C. difficile revealed cell lysis and evidence of defective cell division and spore formation. These studies indicate that rhodomyrtone should be further investigated as a potential treatment for CDI.

Authors+Show Affiliations

Department of Microbiology and Excellence Research Laboratory on Natural Products, Faculty of Science and Natural Products Research Center of Excellence, Prince of Songkla University, Thailand.Department of Microbiology, Monash University, Clayton, Victoria, Australia.Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.Department of Microbiology, Monash University, Clayton, Victoria, Australia.Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia.Department of Microbiology and Excellence Research Laboratory on Natural Products, Faculty of Science and Natural Products Research Center of Excellence, Prince of Songkla University, Thailand.Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Melbourne, Victoria, Australia; Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia. Electronic address: r.browne@unimelb.edu.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30145248

Citation

Srisuwan, Sutthirat, et al. "Antibacterial Activity of Rhodomyrtone On Clostridium Difficile Vegetative Cells and Spores in Vitro." International Journal of Antimicrobial Agents, vol. 52, no. 5, 2018, pp. 724-729.
Srisuwan S, Mackin KE, Hocking D, et al. Antibacterial activity of rhodomyrtone on Clostridium difficile vegetative cells and spores in vitro. Int J Antimicrob Agents. 2018;52(5):724-729.
Srisuwan, S., Mackin, K. E., Hocking, D., Lyras, D., Bennett-Wood, V., Voravuthikunchai, S. P., & Robins-Browne, R. M. (2018). Antibacterial activity of rhodomyrtone on Clostridium difficile vegetative cells and spores in vitro. International Journal of Antimicrobial Agents, 52(5), 724-729. https://doi.org/10.1016/j.ijantimicag.2018.08.014
Srisuwan S, et al. Antibacterial Activity of Rhodomyrtone On Clostridium Difficile Vegetative Cells and Spores in Vitro. Int J Antimicrob Agents. 2018;52(5):724-729. PubMed PMID: 30145248.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial activity of rhodomyrtone on Clostridium difficile vegetative cells and spores in vitro. AU - Srisuwan,Sutthirat, AU - Mackin,Kate E, AU - Hocking,Dianna, AU - Lyras,Dena, AU - Bennett-Wood,Vicki, AU - Voravuthikunchai,Supayang P, AU - Robins-Browne,Roy M, Y1 - 2018/08/24/ PY - 2017/10/23/received PY - 2018/08/04/revised PY - 2018/08/18/accepted PY - 2018/8/27/pubmed PY - 2019/3/21/medline PY - 2018/8/27/entrez KW - Clostridium difficile KW - Rhodomyrtone KW - Spores KW - Vancomycin SP - 724 EP - 729 JF - International journal of antimicrobial agents JO - Int. J. Antimicrob. Agents VL - 52 IS - 5 N2 - The increasing incidence and severity of diarrhoea and colitis caused by Clostridium difficile, together with a high rate of relapse following treatment with currently recommended antimicrobials, calls for novel interventions for C. difficile infection (CDI). Rhodomyrtone, a bioactive compound derived from the leaves of the rose myrtle (Rhodomyrtus tomentosa) has demonstrated antibacterial activity against several Gram-positive bacteria. This study compared the in vitro antimicrobial activity of rhodomyrtone on C. difficile with that of vancomycin, a recommended agent for the treatment of CDI. Determination of the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of rhodomyrtone and vancomycin for ten C. difficile isolates showed that the MICs of rhodomyrtone for C. difficile vegetative cells (0.625-2.5 mg/L) were comparable with that of vancomycin (1.25 mg/L), but the MBCs of rhodomyrtone (1.25-5 mg/L) were significantly lower than those for vancomycin (5 mg/L to ˃40 mg/L; P < 0.001). Time-kill assays showed rapid bactericidal activity for rhodomyrtone, with ≥99% killing within 4 h. Rhodomyrtone was also four-fold more potent than vancomycin in inhibiting C. difficile spore outgrowth. Transmission electron microscopy of rhodomyrtone-treated C. difficile revealed cell lysis and evidence of defective cell division and spore formation. These studies indicate that rhodomyrtone should be further investigated as a potential treatment for CDI. SN - 1872-7913 UR - https://www.unboundmedicine.com/medline/citation/30145248/Antibacterial_activity_of_rhodomyrtone_on_Clostridium_difficile_vegetative_cells_and_spores_in_vitro_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(18)30240-1 DB - PRIME DP - Unbound Medicine ER -