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Generation of trichogenic adipose-derived stem cells by expression of three factors.
J Dermatol Sci. 2018 Oct; 92(1):18-29.JD

Abstract

BACKGROUND

Previous studies demonstrated that adipose-derived stem cells (ASCs) can promote hair growth, but unmet needs exist for enhancing ASC hair inductivity.

OBJECTIVE

Therefore, we introduced three trichogenic factors platelet-derived growth factor-A, SOX2, and β-catenin to ASCs (tfASCs) and evaluated whether tfASCs have similar characteristics as dermal papilla (DP) cells.

METHOD

Global gene expression was examined using NGS analysis. Telogen-to-anagen induction, vibrissae hair follicle organ culture and patch assay were used.

RESULTS

tfASC cell size is smaller than that of ASCs, and they exhibit short doubling time. tfASCs also resist aging and can be expanded until passage 12. Cell proportion in S and G2/M increases in tfASCs, and tfASCs express high mRNA levels of cell cycle related genes. The mRNA expression of DP markers was notably higher in tfASCs. Moreover, NGS analysis revealed that the global gene expression of tfASCs is similar to that of DP cells. The injection of tfASCs accelerated the telogen-to-anagen transition and conditioned medium of tfASCs increased the anagen phase of vibrissal hair follicles. Finally, we found that the injection of 3D-cultured tfASCs at p 9 generated new hair follicles in nude mice.

CONCLUSION

Collectively, these results indicate that 1) tfASCs have similar characteristics as DP cells, 2) tfASCs have enhanced hair-regenerative potential compared with ASCs, and 3) tfASCs even at late passage can make new hair follicles in a hair reconstitution assay. Because DP cells are difficult to isolate/expand and ASCs have low hair inductivity, tfASCs and tfASC-CM are clinically good candidates for hair regeneration.

Authors+Show Affiliations

College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, South Korea; STEMORE Co. Ltd., Incheon, South Korea.College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, South Korea.STEMORE Co. Ltd., Incheon, South Korea.STEMORE Co. Ltd., Incheon, South Korea.College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, South Korea.College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, South Korea.Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine, Seoul, South Korea.College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, South Korea. Electronic address: jinulee@yonsei.ac.kr.College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, South Korea; STEMORE Co. Ltd., Incheon, South Korea. Electronic address: brian99@empal.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30146106

Citation

Choi, Nahyun, et al. "Generation of Trichogenic Adipose-derived Stem Cells By Expression of Three Factors." Journal of Dermatological Science, vol. 92, no. 1, 2018, pp. 18-29.
Choi N, Choi J, Kim JH, et al. Generation of trichogenic adipose-derived stem cells by expression of three factors. J Dermatol Sci. 2018;92(1):18-29.
Choi, N., Choi, J., Kim, J. H., Jang, Y., Yeo, J. H., Kang, J., Song, S. Y., Lee, J., & Sung, J. H. (2018). Generation of trichogenic adipose-derived stem cells by expression of three factors. Journal of Dermatological Science, 92(1), 18-29. https://doi.org/10.1016/j.jdermsci.2018.08.004
Choi N, et al. Generation of Trichogenic Adipose-derived Stem Cells By Expression of Three Factors. J Dermatol Sci. 2018;92(1):18-29. PubMed PMID: 30146106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of trichogenic adipose-derived stem cells by expression of three factors. AU - Choi,Nahyun, AU - Choi,Junjeong, AU - Kim,Ji Hye, AU - Jang,Yaeji, AU - Yeo,Joo Hye, AU - Kang,Juwon, AU - Song,Seung Yong, AU - Lee,Jinu, AU - Sung,Jong-Hyuk, Y1 - 2018/08/16/ PY - 2018/02/27/received PY - 2018/08/07/revised PY - 2018/08/10/accepted PY - 2018/8/28/pubmed PY - 2019/1/8/medline PY - 2018/8/28/entrez KW - Adipose-derived stem cells KW - Dermal papilla cell KW - Hair inductivity KW - Platelet-derived growth factor-A KW - Sox2 KW - β-catenin SP - 18 EP - 29 JF - Journal of dermatological science JO - J Dermatol Sci VL - 92 IS - 1 N2 - BACKGROUND: Previous studies demonstrated that adipose-derived stem cells (ASCs) can promote hair growth, but unmet needs exist for enhancing ASC hair inductivity. OBJECTIVE: Therefore, we introduced three trichogenic factors platelet-derived growth factor-A, SOX2, and β-catenin to ASCs (tfASCs) and evaluated whether tfASCs have similar characteristics as dermal papilla (DP) cells. METHOD: Global gene expression was examined using NGS analysis. Telogen-to-anagen induction, vibrissae hair follicle organ culture and patch assay were used. RESULTS: tfASC cell size is smaller than that of ASCs, and they exhibit short doubling time. tfASCs also resist aging and can be expanded until passage 12. Cell proportion in S and G2/M increases in tfASCs, and tfASCs express high mRNA levels of cell cycle related genes. The mRNA expression of DP markers was notably higher in tfASCs. Moreover, NGS analysis revealed that the global gene expression of tfASCs is similar to that of DP cells. The injection of tfASCs accelerated the telogen-to-anagen transition and conditioned medium of tfASCs increased the anagen phase of vibrissal hair follicles. Finally, we found that the injection of 3D-cultured tfASCs at p 9 generated new hair follicles in nude mice. CONCLUSION: Collectively, these results indicate that 1) tfASCs have similar characteristics as DP cells, 2) tfASCs have enhanced hair-regenerative potential compared with ASCs, and 3) tfASCs even at late passage can make new hair follicles in a hair reconstitution assay. Because DP cells are difficult to isolate/expand and ASCs have low hair inductivity, tfASCs and tfASC-CM are clinically good candidates for hair regeneration. SN - 1873-569X UR - https://www.unboundmedicine.com/medline/citation/30146106/Generation_of_trichogenic_adipose_derived_stem_cells_by_expression_of_three_factors_ DB - PRIME DP - Unbound Medicine ER -