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Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy.
J Pregnancy 2018; 2018:4382084JP

Abstract

Aim

To investigate the changes in the maternal immune system at term pregnancy, we studied the expression of natural cytotoxicity receptors (NCRs) and the cytokine production of NK cells in term placenta decidua and peripheral blood.

Methods

Term decidua and peripheral blood were taken from patients undergoing elective cesarean section. The lymphocytes were separated using density gradient centrifugation (DGC) from peripheral blood and were separated from decidua using DGC after enzyme digestion. These cells were stained with FITC anti-CD56 and Per-CP anti-CD3 monoclonal antibodies, and the NCRs were stained with PE-conjugated anti-NKG2D, NKp46, NKp30, and NKp44 monoclonal antibodies. Cytokines, including IFN-γ, TNF-α, IL-10, and TGF-β, were stained and then analyzed by flow cytometry.

Results

There were fewer cells positive for NKG2D, NKp46, and NKp30 among CD56+CD3- cells in deciduas than in peripheral blood, but the percentages of NKp44-positive cells in CD56+CD3- lymphocytes in deciduas tended to be higher.

Conclusion

The decreased expression of some NCRs in deciduas may be related to decreased cytotoxicity at term pregnancy, but the increased expression of NKp44 may affect the increased cytokine production in the decidua. Similarly, the expression of NCRs in the decidua may be connected to the maintenance of pregnancy at term.

Authors+Show Affiliations

Department of Obstetrics & Gynecology, School of Medicine, Nihon University, Tokyo, Japan.Department of Obstetrics & Gynecology, School of Medicine, Nihon University, Tokyo, Japan.Department of Obstetrics & Gynecology, School of Medicine, Nihon University, Tokyo, Japan.Department of Obstetrics & Gynecology, School of Medicine, Nihon University, Tokyo, Japan.Department of Obstetrics & Gynecology, School of Medicine, Nihon University, Tokyo, Japan.Department of Obstetrics & Gynecology, School of Medicine, Nihon University, Tokyo, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30155304

Citation

Takahashi, Hideki, et al. "Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy." Journal of Pregnancy, vol. 2018, 2018, p. 4382084.
Takahashi H, Yamamoto T, Yamazaki M, et al. Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy. J Pregnancy. 2018;2018:4382084.
Takahashi, H., Yamamoto, T., Yamazaki, M., Murase, T., Matsuno, T., & Chishima, F. (2018). Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy. Journal of Pregnancy, 2018, p. 4382084. doi:10.1155/2018/4382084.
Takahashi H, et al. Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy. J Pregnancy. 2018;2018:4382084. PubMed PMID: 30155304.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy. AU - Takahashi,Hideki, AU - Yamamoto,Tatsuo, AU - Yamazaki,Motomi, AU - Murase,Takayuki, AU - Matsuno,Takayuki, AU - Chishima,Fumihisa, Y1 - 2018/08/01/ PY - 2017/11/11/received PY - 2018/06/24/revised PY - 2018/07/18/accepted PY - 2018/8/30/entrez PY - 2018/8/30/pubmed PY - 2019/1/31/medline SP - 4382084 EP - 4382084 JF - Journal of pregnancy JO - J Pregnancy VL - 2018 N2 - Aim: To investigate the changes in the maternal immune system at term pregnancy, we studied the expression of natural cytotoxicity receptors (NCRs) and the cytokine production of NK cells in term placenta decidua and peripheral blood. Methods: Term decidua and peripheral blood were taken from patients undergoing elective cesarean section. The lymphocytes were separated using density gradient centrifugation (DGC) from peripheral blood and were separated from decidua using DGC after enzyme digestion. These cells were stained with FITC anti-CD56 and Per-CP anti-CD3 monoclonal antibodies, and the NCRs were stained with PE-conjugated anti-NKG2D, NKp46, NKp30, and NKp44 monoclonal antibodies. Cytokines, including IFN-γ, TNF-α, IL-10, and TGF-β, were stained and then analyzed by flow cytometry. Results: There were fewer cells positive for NKG2D, NKp46, and NKp30 among CD56+CD3- cells in deciduas than in peripheral blood, but the percentages of NKp44-positive cells in CD56+CD3- lymphocytes in deciduas tended to be higher. Conclusion: The decreased expression of some NCRs in deciduas may be related to decreased cytotoxicity at term pregnancy, but the increased expression of NKp44 may affect the increased cytokine production in the decidua. Similarly, the expression of NCRs in the decidua may be connected to the maintenance of pregnancy at term. SN - 2090-2735 UR - https://www.unboundmedicine.com/medline/citation/30155304/Natural_Cytotoxicity_Receptors_in_Decidua_Natural_Killer_Cells_of_Term_Normal_Pregnancy_ L2 - https://dx.doi.org/10.1155/2018/4382084 DB - PRIME DP - Unbound Medicine ER -