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Generation of superoxide anion and hydrogen peroxide by erythrocytes from individuals with sickle trait or normal haemoglobin.

Abstract

To test the hypothesis that the resistance of sickle trait (AS Hgb) erythrocytes (rbcs) to malaria may be mediated by increased production of activated oxygen species, the production of superoxide anion (O2-) and hydrogen peroxide (H2O2) by AS rbcs and normal (AA Hgb) rbcs was measured under defined conditions. Formation of O2- and H2O2 was time, temperature and oxygen saturation dependent. Reproducible measurement of O2- formation required the presence of 0.2 mmol l-1 KCN to inhibit a cytochrome oxidase activity found in the cytochrome C preparation used. There was an inverse relationship between cell concentration and O2- and H2O2 formation. Use of the inhibitor of superoxide dismutase (SOD), diethyldithiocarbamic acid, increased the amount of O2- measured. When rbcs from blacks with AS Hgb and with AA Hgb were incubated under standardized conditions, significantly (P less than 0.05) more O2- was formed by AS than AA cells (24.3 v. 14.5 mmol per mol Hgb). These findings show that AS rbcs can generate more O2- than AA rbcs. The increased formation of O2- by rbcs containing AS Hgb may contribute to the resistance of AS rbcs to malarial parasitism.

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    Source

    MeSH

    Adult
    Anemia, Sickle Cell
    Catalase
    Erythrocytes
    Free Radicals
    Hemoglobin A
    Hemoglobin, Sickle
    Humans
    Hydrogen Peroxide
    In Vitro Techniques
    Malaria
    Potassium Cyanide
    Sickle Cell Trait
    Superoxide Dismutase
    Superoxides

    Pub Type(s)

    Journal Article
    Research Support, U.S. Gov't, Non-P.H.S.

    Language

    eng

    PubMed ID

    3015634

    Citation

    Schacter, L P.. "Generation of Superoxide Anion and Hydrogen Peroxide By Erythrocytes From Individuals With Sickle Trait or Normal Haemoglobin." European Journal of Clinical Investigation, vol. 16, no. 3, 1986, pp. 204-10.
    Schacter LP. Generation of superoxide anion and hydrogen peroxide by erythrocytes from individuals with sickle trait or normal haemoglobin. Eur J Clin Invest. 1986;16(3):204-10.
    Schacter, L. P. (1986). Generation of superoxide anion and hydrogen peroxide by erythrocytes from individuals with sickle trait or normal haemoglobin. European Journal of Clinical Investigation, 16(3), pp. 204-10.
    Schacter LP. Generation of Superoxide Anion and Hydrogen Peroxide By Erythrocytes From Individuals With Sickle Trait or Normal Haemoglobin. Eur J Clin Invest. 1986;16(3):204-10. PubMed PMID: 3015634.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Generation of superoxide anion and hydrogen peroxide by erythrocytes from individuals with sickle trait or normal haemoglobin. A1 - Schacter,L P, PY - 1986/6/1/pubmed PY - 1986/6/1/medline PY - 1986/6/1/entrez SP - 204 EP - 10 JF - European journal of clinical investigation JO - Eur. J. Clin. Invest. VL - 16 IS - 3 N2 - To test the hypothesis that the resistance of sickle trait (AS Hgb) erythrocytes (rbcs) to malaria may be mediated by increased production of activated oxygen species, the production of superoxide anion (O2-) and hydrogen peroxide (H2O2) by AS rbcs and normal (AA Hgb) rbcs was measured under defined conditions. Formation of O2- and H2O2 was time, temperature and oxygen saturation dependent. Reproducible measurement of O2- formation required the presence of 0.2 mmol l-1 KCN to inhibit a cytochrome oxidase activity found in the cytochrome C preparation used. There was an inverse relationship between cell concentration and O2- and H2O2 formation. Use of the inhibitor of superoxide dismutase (SOD), diethyldithiocarbamic acid, increased the amount of O2- measured. When rbcs from blacks with AS Hgb and with AA Hgb were incubated under standardized conditions, significantly (P less than 0.05) more O2- was formed by AS than AA cells (24.3 v. 14.5 mmol per mol Hgb). These findings show that AS rbcs can generate more O2- than AA rbcs. The increased formation of O2- by rbcs containing AS Hgb may contribute to the resistance of AS rbcs to malarial parasitism. SN - 0014-2972 UR - https://www.unboundmedicine.com/medline/citation/3015634/Generation_of_superoxide_anion_and_hydrogen_peroxide_by_erythrocytes_from_individuals_with_sickle_trait_or_normal_haemoglobin_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0014-2972&date=1986&volume=16&issue=3&spage=204 DB - PRIME DP - Unbound Medicine ER -