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Generation of superoxide anion and hydrogen peroxide by erythrocytes from individuals with sickle trait or normal haemoglobin.
Eur J Clin Invest 1986; 16(3):204-10EJ

Abstract

To test the hypothesis that the resistance of sickle trait (AS Hgb) erythrocytes (rbcs) to malaria may be mediated by increased production of activated oxygen species, the production of superoxide anion (O2-) and hydrogen peroxide (H2O2) by AS rbcs and normal (AA Hgb) rbcs was measured under defined conditions. Formation of O2- and H2O2 was time, temperature and oxygen saturation dependent. Reproducible measurement of O2- formation required the presence of 0.2 mmol l-1 KCN to inhibit a cytochrome oxidase activity found in the cytochrome C preparation used. There was an inverse relationship between cell concentration and O2- and H2O2 formation. Use of the inhibitor of superoxide dismutase (SOD), diethyldithiocarbamic acid, increased the amount of O2- measured. When rbcs from blacks with AS Hgb and with AA Hgb were incubated under standardized conditions, significantly (P less than 0.05) more O2- was formed by AS than AA cells (24.3 v. 14.5 mmol per mol Hgb). These findings show that AS rbcs can generate more O2- than AA rbcs. The increased formation of O2- by rbcs containing AS Hgb may contribute to the resistance of AS rbcs to malarial parasitism.

Authors

No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

3015634

Citation

Schacter, L P.. "Generation of Superoxide Anion and Hydrogen Peroxide By Erythrocytes From Individuals With Sickle Trait or Normal Haemoglobin." European Journal of Clinical Investigation, vol. 16, no. 3, 1986, pp. 204-10.
Schacter LP. Generation of superoxide anion and hydrogen peroxide by erythrocytes from individuals with sickle trait or normal haemoglobin. Eur J Clin Invest. 1986;16(3):204-10.
Schacter, L. P. (1986). Generation of superoxide anion and hydrogen peroxide by erythrocytes from individuals with sickle trait or normal haemoglobin. European Journal of Clinical Investigation, 16(3), pp. 204-10.
Schacter LP. Generation of Superoxide Anion and Hydrogen Peroxide By Erythrocytes From Individuals With Sickle Trait or Normal Haemoglobin. Eur J Clin Invest. 1986;16(3):204-10. PubMed PMID: 3015634.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of superoxide anion and hydrogen peroxide by erythrocytes from individuals with sickle trait or normal haemoglobin. A1 - Schacter,L P, PY - 1986/6/1/pubmed PY - 1986/6/1/medline PY - 1986/6/1/entrez SP - 204 EP - 10 JF - European journal of clinical investigation JO - Eur. J. Clin. Invest. VL - 16 IS - 3 N2 - To test the hypothesis that the resistance of sickle trait (AS Hgb) erythrocytes (rbcs) to malaria may be mediated by increased production of activated oxygen species, the production of superoxide anion (O2-) and hydrogen peroxide (H2O2) by AS rbcs and normal (AA Hgb) rbcs was measured under defined conditions. Formation of O2- and H2O2 was time, temperature and oxygen saturation dependent. Reproducible measurement of O2- formation required the presence of 0.2 mmol l-1 KCN to inhibit a cytochrome oxidase activity found in the cytochrome C preparation used. There was an inverse relationship between cell concentration and O2- and H2O2 formation. Use of the inhibitor of superoxide dismutase (SOD), diethyldithiocarbamic acid, increased the amount of O2- measured. When rbcs from blacks with AS Hgb and with AA Hgb were incubated under standardized conditions, significantly (P less than 0.05) more O2- was formed by AS than AA cells (24.3 v. 14.5 mmol per mol Hgb). These findings show that AS rbcs can generate more O2- than AA rbcs. The increased formation of O2- by rbcs containing AS Hgb may contribute to the resistance of AS rbcs to malarial parasitism. SN - 0014-2972 UR - https://www.unboundmedicine.com/medline/citation/3015634/Generation_of_superoxide_anion_and_hydrogen_peroxide_by_erythrocytes_from_individuals_with_sickle_trait_or_normal_haemoglobin_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0014-2972&date=1986&volume=16&issue=3&spage=204 DB - PRIME DP - Unbound Medicine ER -