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Molecular markers and cytogenetics to characterize a wheat-Dasypyrum villosum 3V (3D) substitution line conferring resistance to stripe rust.
PLoS One. 2018; 13(8):e0202033.Plos

Abstract

Dasypyrum villosum has been used as a valuable gene resource for disease resistances, yield increase and quality improvement in wheat. A novel wheat-D. villosum alien introgression line CD-3 was generated through hybridization between the common wheat Chinese Spring (CS) and a CS- D. villosum 3V addition line having considerably high stripe rust resistance, which enable the characterization of a potential new stripe rust resistance gene (s) derived from D. villosum. The results of non-denaturing fluorescent in situ hybridization (ND-FISH) showed that CD-3 contained 42 chromosomes, including a 3V chromosome pair, and the absence of both of the 3D chromosomes. PCR-based Landmark Unique Gene (PLUG) molecular marker analysis supported results from the FISH analysis, revealing CD-3 was a wheat-D. villosum 3V (3D) disomic substitution line. Resistant test of stripe rust on 52 plants of F2 generation (CD-3/CS), CD-3, CS and D.villosum have been conducted at seedling stage. 7 plants of F2 generation possessing two 3V chromosomes exhibited high resistance to stripe rust as CD-3 and D.villosum, 10 plants carrying one 3V chromosome and 35 plants without 3V chromosome were susceptive to stripe rust as CS. The result implied the high stripe rust resistance of CD-3 should be controlled by recessive gene(s) originating from D.villosum. To rapidly detect chromosome 3V in the genetic background of wheat, we developed a novel Sequence Characterized Amplified Region (SCAR) marker specific for 3V chromosome based on the sequence of a grain size-related gene DvGS5 in D. villosum, an orthologue of TaGS5 from wheat. The SCAR marker was designated DvGS5-1443, which could successfully amplify a unique 3V-specific fragment in CD-3 and D. villosum, suggesting that this SCAR marker could facilitate targeting the chromosome 3V in the genetic background of wheat for wheat improvement.

Authors+Show Affiliations

Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, China. Key Laboratory of Wheat Biology and Genetic Improvement on Southwestern China (Ministry of Agriculture), Chengdu, Sichuan, China.Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, China.Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, China.Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, China.Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, China.Institute of Agro-products Processing Science and Technology, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30157196

Citation

Zhang, Jie, et al. "Molecular Markers and Cytogenetics to Characterize a wheat-Dasypyrum Villosum 3V (3D) Substitution Line Conferring Resistance to Stripe Rust." PloS One, vol. 13, no. 8, 2018, pp. e0202033.
Zhang J, Jiang Y, Wang Y, et al. Molecular markers and cytogenetics to characterize a wheat-Dasypyrum villosum 3V (3D) substitution line conferring resistance to stripe rust. PLoS One. 2018;13(8):e0202033.
Zhang, J., Jiang, Y., Wang, Y., Guo, Y., Long, H., Deng, G., Chen, Q., & Xuan, P. (2018). Molecular markers and cytogenetics to characterize a wheat-Dasypyrum villosum 3V (3D) substitution line conferring resistance to stripe rust. PloS One, 13(8), e0202033. https://doi.org/10.1371/journal.pone.0202033
Zhang J, et al. Molecular Markers and Cytogenetics to Characterize a wheat-Dasypyrum Villosum 3V (3D) Substitution Line Conferring Resistance to Stripe Rust. PLoS One. 2018;13(8):e0202033. PubMed PMID: 30157196.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular markers and cytogenetics to characterize a wheat-Dasypyrum villosum 3V (3D) substitution line conferring resistance to stripe rust. AU - Zhang,Jie, AU - Jiang,Yun, AU - Wang,Ying, AU - Guo,Yuanlin, AU - Long,Hai, AU - Deng,Guangbing, AU - Chen,Qian, AU - Xuan,Pu, Y1 - 2018/08/29/ PY - 2018/02/21/received PY - 2018/07/26/accepted PY - 2018/8/30/entrez PY - 2018/8/30/pubmed PY - 2019/2/1/medline SP - e0202033 EP - e0202033 JF - PloS one JO - PLoS One VL - 13 IS - 8 N2 - Dasypyrum villosum has been used as a valuable gene resource for disease resistances, yield increase and quality improvement in wheat. A novel wheat-D. villosum alien introgression line CD-3 was generated through hybridization between the common wheat Chinese Spring (CS) and a CS- D. villosum 3V addition line having considerably high stripe rust resistance, which enable the characterization of a potential new stripe rust resistance gene (s) derived from D. villosum. The results of non-denaturing fluorescent in situ hybridization (ND-FISH) showed that CD-3 contained 42 chromosomes, including a 3V chromosome pair, and the absence of both of the 3D chromosomes. PCR-based Landmark Unique Gene (PLUG) molecular marker analysis supported results from the FISH analysis, revealing CD-3 was a wheat-D. villosum 3V (3D) disomic substitution line. Resistant test of stripe rust on 52 plants of F2 generation (CD-3/CS), CD-3, CS and D.villosum have been conducted at seedling stage. 7 plants of F2 generation possessing two 3V chromosomes exhibited high resistance to stripe rust as CD-3 and D.villosum, 10 plants carrying one 3V chromosome and 35 plants without 3V chromosome were susceptive to stripe rust as CS. The result implied the high stripe rust resistance of CD-3 should be controlled by recessive gene(s) originating from D.villosum. To rapidly detect chromosome 3V in the genetic background of wheat, we developed a novel Sequence Characterized Amplified Region (SCAR) marker specific for 3V chromosome based on the sequence of a grain size-related gene DvGS5 in D. villosum, an orthologue of TaGS5 from wheat. The SCAR marker was designated DvGS5-1443, which could successfully amplify a unique 3V-specific fragment in CD-3 and D. villosum, suggesting that this SCAR marker could facilitate targeting the chromosome 3V in the genetic background of wheat for wheat improvement. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/30157196/Molecular_markers_and_cytogenetics_to_characterize_a_wheat_Dasypyrum_villosum_3V__3D__substitution_line_conferring_resistance_to_stripe_rust_ L2 - https://dx.plos.org/10.1371/journal.pone.0202033 DB - PRIME DP - Unbound Medicine ER -