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Detection and phylogenetic analysis of Orf virus in Kashmir Himalayas.
Virusdisease 2018; 29(3):405-410V

Abstract

Orf virus (ORFV) is a zoonotic pathogen that primarily infects sheep and goats, and is responsible for significant economic losses. ORFV is endemic in all the major sheep and goat rearing areas of the world including Indian subcontinent. However, the nature of ORFV circulating among sheep and goat in Kashmir Himalayas has not yet been characterized. In the present study, we describe natural outbreaks of ORFV in sheep and goats of Kashmir Himalayas. We detected the presence of ORFV in the scab lesion by PCR amplification of the major envelope protein (B2L) gene. We sequenced the virus interferon resistance (VIR) gene and determined their phylogenetic relationship with that of the published reference sequences. Phylogenetic analysis based on VIR gene revealed that the ORFV isolates from Kashmir Himalayas separated into main two clusters. The sheep isolates showed genetic homology with the sheep strains reported from Greece and Italy, whereas the goat-specific strain show homology with the goat strains reported from China. This study demonstrates the presence of ORFV infection in sheep and goats, and report first phylogenetic analysis of the ORFV strains prevalent in the Kashmir Himalayas.

Authors+Show Affiliations

1Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Sciences and Animal Husbandry (FVSc & AH), Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Srinagar, 190006 India.1Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Sciences and Animal Husbandry (FVSc & AH), Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Srinagar, 190006 India.1Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Sciences and Animal Husbandry (FVSc & AH), Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Srinagar, 190006 India.1Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Sciences and Animal Husbandry (FVSc & AH), Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Srinagar, 190006 India.2Faculty of Veterinary Sciences and Animal Husbandry (FVSc & AH), Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Srinagar, 190006 India.2Faculty of Veterinary Sciences and Animal Husbandry (FVSc & AH), Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Srinagar, 190006 India.2Faculty of Veterinary Sciences and Animal Husbandry (FVSc & AH), Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Srinagar, 190006 India.3Center for Cellular and Molecular Biology, Hyderabad, Telangana 500007 India.1Division of Veterinary Microbiology and Immunology, Faculty of Veterinary Sciences and Animal Husbandry (FVSc & AH), Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Srinagar, 190006 India. 4Present Address: Department of Medicine, University of Toledo, Toledo, OH USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30159380

Citation

Ahanger, Showket A., et al. "Detection and Phylogenetic Analysis of Orf Virus in Kashmir Himalayas." Virusdisease, vol. 29, no. 3, 2018, pp. 405-410.
Ahanger SA, Parveen R, Nazki S, et al. Detection and phylogenetic analysis of Orf virus in Kashmir Himalayas. Virusdisease. 2018;29(3):405-410.
Ahanger, S. A., Parveen, R., Nazki, S., Dar, Z., Dar, T., Dar, K. H., ... Dar, P. (2018). Detection and phylogenetic analysis of Orf virus in Kashmir Himalayas. Virusdisease, 29(3), pp. 405-410. doi:10.1007/s13337-018-0473-1.
Ahanger SA, et al. Detection and Phylogenetic Analysis of Orf Virus in Kashmir Himalayas. Virusdisease. 2018;29(3):405-410. PubMed PMID: 30159380.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection and phylogenetic analysis of Orf virus in Kashmir Himalayas. AU - Ahanger,Showket A, AU - Parveen,Rafia, AU - Nazki,Salik, AU - Dar,Zahoor, AU - Dar,Tanveer, AU - Dar,Khadim Hussain, AU - Dar,Aijaz, AU - Rai,Niraj, AU - Dar,Pervaiz, Y1 - 2018/07/28/ PY - 2017/11/23/received PY - 2018/06/28/accepted PY - 2018/8/31/entrez PY - 2018/8/31/pubmed PY - 2018/8/31/medline KW - Contagious ecthyma KW - Goat KW - Orf virus KW - Phylogenetic analysis KW - Sheep KW - Virus Interferon Resistance (VIR) gene SP - 405 EP - 410 JF - Virusdisease JO - Virusdisease VL - 29 IS - 3 N2 - Orf virus (ORFV) is a zoonotic pathogen that primarily infects sheep and goats, and is responsible for significant economic losses. ORFV is endemic in all the major sheep and goat rearing areas of the world including Indian subcontinent. However, the nature of ORFV circulating among sheep and goat in Kashmir Himalayas has not yet been characterized. In the present study, we describe natural outbreaks of ORFV in sheep and goats of Kashmir Himalayas. We detected the presence of ORFV in the scab lesion by PCR amplification of the major envelope protein (B2L) gene. We sequenced the virus interferon resistance (VIR) gene and determined their phylogenetic relationship with that of the published reference sequences. Phylogenetic analysis based on VIR gene revealed that the ORFV isolates from Kashmir Himalayas separated into main two clusters. The sheep isolates showed genetic homology with the sheep strains reported from Greece and Italy, whereas the goat-specific strain show homology with the goat strains reported from China. This study demonstrates the presence of ORFV infection in sheep and goats, and report first phylogenetic analysis of the ORFV strains prevalent in the Kashmir Himalayas. SN - 2347-3584 UR - https://www.unboundmedicine.com/medline/citation/30159380/Detection_and_phylogenetic_analysis_of_Orf_virus_in_Kashmir_Himalayas_ L2 - https://dx.doi.org/10.1007/s13337-018-0473-1 DB - PRIME DP - Unbound Medicine ER -