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Rhubarb Peony Decoction ameliorates ulcerative colitis in mice by regulating gut microbiota to restoring Th17/Treg balance.
J Ethnopharmacol 2019; 231:39-49JE

Abstract

ETHNOPHARMACOLOGY RELEVANCE

Rhubarb Peony Decoction (RPD) is a formula of traditional Chinese medicine chronicled in Jin Gui Yao Lve, commonly used to treat ulcerative colitis (UC). However, the underlying mechanism of RPD treating UC remains elusive. In our study, we investigated the therapeutic efficacy of RPD and potential mechanism involved in inhibiting dextran sulfate sodium (DSS)-induced ulcerative colitis in mice.

METHODS

The colitis was induced by DSS in mice for 5 days and estimated body weight loss, disease activity index (DAI) and colon length. Histological changes were observed by H&E staining. The number and abundance of gut mircrobiota were measured with 16 S rDNA sequencing. GC-MS was used to detect the concentration of short chain fatty acids (SCFAs) in cecum. Flow cytometry analyzed the proportion of Th17 and Treg cells in mesenteric lymph nodes (MLNs). IL-17A and Foxp3 in colon were determined by immunohistochemical analyses. The level of cytokine was determined by Multi-Analyte Flow Assay Kit.

RESULTS

Administration of RPD significantly alleviated the pathological changes of UC mice, involving rescued the inflammation-related reduction of colon length, ameliorated body weight loss and damaged tissue. In addition, RPD altered the gut microbiota, involving restored α diversity, increased significantly the abundance of Firmicutes and Actinobacteria, decreased the Proteobacteria and Bacteroidetes. Furthermore, the number of Butyricicoccus pullicaecorum, a butyrate-producing bacterium, were augmented obviously by RPD. Besides, RPD restored the content of SCFA in intestinal tract. Additionally, the proportion of Th17 cells and Treg cells in mesenteric lymph nodes, likewise, the expression of IL-17A and Foxp3 in colon were regulated by RPD, contributing to the restoration of Th17/Treg balance. Moreover, RPD significantly decreased the level of IL-6, TNF-α, IFNγ, IL-10, IL-17A, IL-21, IL-22 in colon, simultaneously increased Treg-related cytokine TGF-β at dose-dependently.

CONCLUSIONS

These results demonstrated that RPD had effect on ulcerative colitis, which was related to regulating gut microbiota, especially Butyricicoccus pullicaecorum, and SCFAs to restore the gut Th17/Treg homeostasis.

Authors+Show Affiliations

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.Guangxi Scientific Experimental Center of Traditional Chinese Medicine, Guangxi University of Chinese Medicine, Nanning, China.School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.Basic Medical College, Guangzhou University of Chinese Medicine, Guangzhou, China.School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address: zl@gzucm.edu.cn.School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China. Electronic address: luoxia@gzucm.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30170079

Citation

Luo, Shuang, et al. "Rhubarb Peony Decoction Ameliorates Ulcerative Colitis in Mice By Regulating Gut Microbiota to Restoring Th17/Treg Balance." Journal of Ethnopharmacology, vol. 231, 2019, pp. 39-49.
Luo S, Wen R, Wang Q, et al. Rhubarb Peony Decoction ameliorates ulcerative colitis in mice by regulating gut microbiota to restoring Th17/Treg balance. J Ethnopharmacol. 2019;231:39-49.
Luo, S., Wen, R., Wang, Q., Zhao, Z., Nong, F., Fu, Y., ... Luo, X. (2019). Rhubarb Peony Decoction ameliorates ulcerative colitis in mice by regulating gut microbiota to restoring Th17/Treg balance. Journal of Ethnopharmacology, 231, pp. 39-49. doi:10.1016/j.jep.2018.08.033.
Luo S, et al. Rhubarb Peony Decoction Ameliorates Ulcerative Colitis in Mice By Regulating Gut Microbiota to Restoring Th17/Treg Balance. J Ethnopharmacol. 2019 Mar 1;231:39-49. PubMed PMID: 30170079.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rhubarb Peony Decoction ameliorates ulcerative colitis in mice by regulating gut microbiota to restoring Th17/Treg balance. AU - Luo,Shuang, AU - Wen,Ruyan, AU - Wang,Qing, AU - Zhao,Zhongxiang, AU - Nong,Feifei, AU - Fu,Yajun, AU - Huang,Shaowei, AU - Chen,Jinyan, AU - Zhou,Lian, AU - Luo,Xia, Y1 - 2018/08/28/ PY - 2018/05/08/received PY - 2018/08/16/revised PY - 2018/08/26/accepted PY - 2018/9/1/pubmed PY - 2019/2/26/medline PY - 2018/9/1/entrez KW - Butyricicoccus pullicaecorum KW - Gut microbiota KW - Rhubarb Peony Decoction KW - SCFAs KW - Th17/Treg KW - Ulcerative colitis SP - 39 EP - 49 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 231 N2 - ETHNOPHARMACOLOGY RELEVANCE: Rhubarb Peony Decoction (RPD) is a formula of traditional Chinese medicine chronicled in Jin Gui Yao Lve, commonly used to treat ulcerative colitis (UC). However, the underlying mechanism of RPD treating UC remains elusive. In our study, we investigated the therapeutic efficacy of RPD and potential mechanism involved in inhibiting dextran sulfate sodium (DSS)-induced ulcerative colitis in mice. METHODS: The colitis was induced by DSS in mice for 5 days and estimated body weight loss, disease activity index (DAI) and colon length. Histological changes were observed by H&E staining. The number and abundance of gut mircrobiota were measured with 16 S rDNA sequencing. GC-MS was used to detect the concentration of short chain fatty acids (SCFAs) in cecum. Flow cytometry analyzed the proportion of Th17 and Treg cells in mesenteric lymph nodes (MLNs). IL-17A and Foxp3 in colon were determined by immunohistochemical analyses. The level of cytokine was determined by Multi-Analyte Flow Assay Kit. RESULTS: Administration of RPD significantly alleviated the pathological changes of UC mice, involving rescued the inflammation-related reduction of colon length, ameliorated body weight loss and damaged tissue. In addition, RPD altered the gut microbiota, involving restored α diversity, increased significantly the abundance of Firmicutes and Actinobacteria, decreased the Proteobacteria and Bacteroidetes. Furthermore, the number of Butyricicoccus pullicaecorum, a butyrate-producing bacterium, were augmented obviously by RPD. Besides, RPD restored the content of SCFA in intestinal tract. Additionally, the proportion of Th17 cells and Treg cells in mesenteric lymph nodes, likewise, the expression of IL-17A and Foxp3 in colon were regulated by RPD, contributing to the restoration of Th17/Treg balance. Moreover, RPD significantly decreased the level of IL-6, TNF-α, IFNγ, IL-10, IL-17A, IL-21, IL-22 in colon, simultaneously increased Treg-related cytokine TGF-β at dose-dependently. CONCLUSIONS: These results demonstrated that RPD had effect on ulcerative colitis, which was related to regulating gut microbiota, especially Butyricicoccus pullicaecorum, and SCFAs to restore the gut Th17/Treg homeostasis. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/30170079/Rhubarb_Peony_Decoction_ameliorates_ulcerative_colitis_in_mice_by_regulating_gut_microbiota_to_restoring_Th17/Treg_balance_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(18)31697-0 DB - PRIME DP - Unbound Medicine ER -