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Evidence of the bioaccumulation of ciguatoxins in giant clams (Tridacna maxima) exposed to Gambierdiscus spp. cells.
Harmful Algae. 2016 Jul; 57(Pt A):78-87.HA

Abstract

Ciguatera Fish Poisoning (CFP) is a foodborne disease classically related to the consumption of tropical coral reef fishes contaminated with ciguatoxins (CTXs), neurotoxins produced by dinoflagellates of the Gambierdiscus genus. Severe atypical ciguatera-like incidents involving giant clams, a marine resource highly consumed in the South Pacific, are also frequently reported in many Pacific Islands Countries and Territories. The present study was designed to assess the ability of giant clams to accumulate CTXs in their tissues and highlight the potential health risks associated with their consumption. Since giant clams are likely to be exposed to both free-swimming Gambierdiscus cells and dissolved CTXs in natural environment, ex situ contamination experiments were conducted as follows: giant clams were exposed to live or lyzed cells of TB92, a highly toxic strain of G. polynesiensis containing 5.83±0.85pg P-CTX-3C equiv.cell-1vs. HIT0, a weakly toxic strain of G. toxicus containing only (2.05±1.16)×10-3pg P-CTX-3C equiv.cell-1, administered over a 48h period at a concentration of 150cellsmL-1. The presence of CTXs in giant clams tissues was further assessed using the mouse neuroblastoma cell-based assay (CBA-N2a). Results showed that giant clams exposed to either lyzed or live cells of TB92 were able to bioaccumulate CTXs at concentrations well above the safety limit recommended for human consumption, i.e. 3.28±1.37 and 2.92±1.03ng P-CTX-3C equiv.g-1 flesh (wet weight), respectively, which represented approximately 3% of the total toxin load administered to the animals. In contrast, giant clams exposed to live or lyzed cells of HIT0 were found to be free of toxins, suggesting that in the nature, the risk of contamination of these bivalves is established only in the presence of highly toxic blooms of Gambierdiscus. Liquid chromatography-mass spectrometry (LC-MS/MS) analyses confirmed CBA-N2a results and also revealed that P-CTX-3B was the major CTX congener retained in the tissues of giant clams fed with TB92 cells. To the best of our knowledge, this study is the first to provide evidence of the bioaccumulation of Gambierdiscus CTXs in giant clams and confirms that these bivalve molluscs can actually constitute another pathway in ciguatera poisonings. While most monitoring programs currently focus on fish toxicity, these findings stress the importance of a concomitant surveillance of these marine invertebrates in applicable locations for an accurate assessment of ciguatera risk.

Authors+Show Affiliations

Institut de Recherche pour le Développement (IRD) - UMR 241-EIO, PO Box 529, 98713 Papeete, Tahiti, French Polynesia. Electronic address: melanie.roue@ird.fr.Institut Louis Malardé (ILM), Laboratory of Toxic Microalgae - UMR 241-EIO, PO Box 30, 98713 Papeete, Tahiti, French Polynesia.Institut de Recherche pour le Développement (IRD) - UMR 241-EIO, PO Box 529, 98713 Papeete, Tahiti, French Polynesia; Institut Louis Malardé (ILM), Laboratory of Toxic Microalgae - UMR 241-EIO, PO Box 30, 98713 Papeete, Tahiti, French Polynesia.Institut Louis Malardé (ILM), Laboratory of Toxic Microalgae - UMR 241-EIO, PO Box 30, 98713 Papeete, Tahiti, French Polynesia.Institut Louis Malardé (ILM), Laboratory of Toxic Microalgae - UMR 241-EIO, PO Box 30, 98713 Papeete, Tahiti, French Polynesia.Université de la Polynésie Française (UPF) - UMR 241-EIO, PO Box 6570, 98702 Faa'a, Tahiti, French Polynesia.Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Phycotoxins Laboratory, PO Box 21105, 44311 Nantes, France.Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Phycotoxins Laboratory, PO Box 21105, 44311 Nantes, France.Institut Louis Malardé (ILM), Laboratory of Toxic Microalgae - UMR 241-EIO, PO Box 30, 98713 Papeete, Tahiti, French Polynesia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30170724

Citation

Roué, Mélanie, et al. "Evidence of the Bioaccumulation of Ciguatoxins in Giant Clams (Tridacna Maxima) Exposed to Gambierdiscus Spp. Cells." Harmful Algae, vol. 57, no. Pt A, 2016, pp. 78-87.
Roué M, Darius HT, Picot S, et al. Evidence of the bioaccumulation of ciguatoxins in giant clams (Tridacna maxima) exposed to Gambierdiscus spp. cells. Harmful Algae. 2016;57(Pt A):78-87.
Roué, M., Darius, H. T., Picot, S., Ung, A., Viallon, J., Gaertner-Mazouni, N., Sibat, M., Amzil, Z., & Chinain, M. (2016). Evidence of the bioaccumulation of ciguatoxins in giant clams (Tridacna maxima) exposed to Gambierdiscus spp. cells. Harmful Algae, 57(Pt A), 78-87. https://doi.org/10.1016/j.hal.2016.05.007
Roué M, et al. Evidence of the Bioaccumulation of Ciguatoxins in Giant Clams (Tridacna Maxima) Exposed to Gambierdiscus Spp. Cells. Harmful Algae. 2016;57(Pt A):78-87. PubMed PMID: 30170724.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of the bioaccumulation of ciguatoxins in giant clams (Tridacna maxima) exposed to Gambierdiscus spp. cells. AU - Roué,Mélanie, AU - Darius,Hélène Taiana, AU - Picot,Sandy, AU - Ung,André, AU - Viallon,Jérôme, AU - Gaertner-Mazouni,Nabila, AU - Sibat,Manoella, AU - Amzil,Zouher, AU - Chinain,Mireille, Y1 - 2016/06/23/ PY - 2016/03/08/received PY - 2016/05/30/revised PY - 2016/05/31/accepted PY - 2018/9/2/entrez PY - 2016/7/1/pubmed PY - 2016/7/1/medline KW - Ciguatoxins KW - Ex situ contamination KW - Gambierdiscus polynesiensis KW - Giant clams KW - LC–MS/MS KW - Neuroblastoma cell-based assay SP - 78 EP - 87 JF - Harmful algae JO - Harmful Algae VL - 57 IS - Pt A N2 - Ciguatera Fish Poisoning (CFP) is a foodborne disease classically related to the consumption of tropical coral reef fishes contaminated with ciguatoxins (CTXs), neurotoxins produced by dinoflagellates of the Gambierdiscus genus. Severe atypical ciguatera-like incidents involving giant clams, a marine resource highly consumed in the South Pacific, are also frequently reported in many Pacific Islands Countries and Territories. The present study was designed to assess the ability of giant clams to accumulate CTXs in their tissues and highlight the potential health risks associated with their consumption. Since giant clams are likely to be exposed to both free-swimming Gambierdiscus cells and dissolved CTXs in natural environment, ex situ contamination experiments were conducted as follows: giant clams were exposed to live or lyzed cells of TB92, a highly toxic strain of G. polynesiensis containing 5.83±0.85pg P-CTX-3C equiv.cell-1vs. HIT0, a weakly toxic strain of G. toxicus containing only (2.05±1.16)×10-3pg P-CTX-3C equiv.cell-1, administered over a 48h period at a concentration of 150cellsmL-1. The presence of CTXs in giant clams tissues was further assessed using the mouse neuroblastoma cell-based assay (CBA-N2a). Results showed that giant clams exposed to either lyzed or live cells of TB92 were able to bioaccumulate CTXs at concentrations well above the safety limit recommended for human consumption, i.e. 3.28±1.37 and 2.92±1.03ng P-CTX-3C equiv.g-1 flesh (wet weight), respectively, which represented approximately 3% of the total toxin load administered to the animals. In contrast, giant clams exposed to live or lyzed cells of HIT0 were found to be free of toxins, suggesting that in the nature, the risk of contamination of these bivalves is established only in the presence of highly toxic blooms of Gambierdiscus. Liquid chromatography-mass spectrometry (LC-MS/MS) analyses confirmed CBA-N2a results and also revealed that P-CTX-3B was the major CTX congener retained in the tissues of giant clams fed with TB92 cells. To the best of our knowledge, this study is the first to provide evidence of the bioaccumulation of Gambierdiscus CTXs in giant clams and confirms that these bivalve molluscs can actually constitute another pathway in ciguatera poisonings. While most monitoring programs currently focus on fish toxicity, these findings stress the importance of a concomitant surveillance of these marine invertebrates in applicable locations for an accurate assessment of ciguatera risk. SN - 1878-1470 UR - https://www.unboundmedicine.com/medline/citation/30170724/Evidence_of_the_bioaccumulation_of_ciguatoxins_in_giant_clams__Tridacna_maxima__exposed_to_Gambierdiscus_spp__cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1568-9883(16)30059-2 DB - PRIME DP - Unbound Medicine ER -
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