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Acute microplastic exposure raises stress response and suppresses detoxification and immune capacities in the scleractinian coral Pocillopora damicornis.
Environ Pollut. 2018 Dec; 243(Pt A):66-74.EP

Abstract

Microplastics are widespread emerging contaminants that have been found globally in the marine and freshwater ecosystem, but there is limited knowledge regarding its impact on coral reef ecosystem and underpinning mechanism. In the present study, using Pocillopora damicornis as a model, we investigated cytological, physiological, and molecular responses of a scleractinian coral to acute microplastic exposure. No significant changes were observed in the density of symbiotic zooxanthellae during the entire period of microplastic exposure, while its chlorophyll content increased significantly at 12 h of microplastic exposure. We observed significant increases in the activities of antioxidant enzymes such as superoxide dismutase and catalase, significant decrease in the detoxifying enzyme glutathione S-transferase and the immune enzyme alkaline phosphatase, but no change in the other immune enzyme phenoloxidase during the whole experiment period. Transcriptomic analysis revealed 134 significantly up-regulated coral genes at 12 h after the exposure, enriched in 11 GO terms mostly related to stress response, zymogen granule, and JNK signal pathway. Meanwhile, 215 coral genes were significantly down-regulated at 12 h after exposure, enriched in 25 GO terms involved in sterol transport and EGF-ERK1/2 signal pathway. In contrast, only 12 zooxanthella genes exhibited significant up-regulation and 95 genes down-regulation at 12 h after the microplastic exposure; genes regulating synthesis and export of glucose and amino acids were not impacted. These results suggest that acute exposure of microplastics can activate the stress response of the scleractinian coral P. damicornis, and repress its detoxification and immune system through the JNK and ERK signal pathways. These demonstrate that microplastic exposure can compromise the anti-stress capacity and immune system of the scleractinian coral P. damicornis, despite the minimal impact on the abundance and major photosynthate translocation transporters of the symbiont in the short term.

Authors+Show Affiliations

Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou, Hainan, China.Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou, Hainan, China.Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou, Hainan, China; State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, Hainan, China; Department of Marine Sciences, University of Connecticut, Groton, CT, USA. Electronic address: zhouzhi@hainu.edu.cn.Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou, Hainan, China; State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, Hainan, China.Department of Marine Sciences, University of Connecticut, Groton, CT, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30172125

Citation

Tang, Jia, et al. "Acute Microplastic Exposure Raises Stress Response and Suppresses Detoxification and Immune Capacities in the Scleractinian Coral Pocillopora Damicornis." Environmental Pollution (Barking, Essex : 1987), vol. 243, no. Pt A, 2018, pp. 66-74.
Tang J, Ni X, Zhou Z, et al. Acute microplastic exposure raises stress response and suppresses detoxification and immune capacities in the scleractinian coral Pocillopora damicornis. Environ Pollut. 2018;243(Pt A):66-74.
Tang, J., Ni, X., Zhou, Z., Wang, L., & Lin, S. (2018). Acute microplastic exposure raises stress response and suppresses detoxification and immune capacities in the scleractinian coral Pocillopora damicornis. Environmental Pollution (Barking, Essex : 1987), 243(Pt A), 66-74. https://doi.org/10.1016/j.envpol.2018.08.045
Tang J, et al. Acute Microplastic Exposure Raises Stress Response and Suppresses Detoxification and Immune Capacities in the Scleractinian Coral Pocillopora Damicornis. Environ Pollut. 2018;243(Pt A):66-74. PubMed PMID: 30172125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Acute microplastic exposure raises stress response and suppresses detoxification and immune capacities in the scleractinian coral Pocillopora damicornis. AU - Tang,Jia, AU - Ni,Xingzhen, AU - Zhou,Zhi, AU - Wang,Lingui, AU - Lin,Senjie, Y1 - 2018/08/20/ PY - 2018/05/11/received PY - 2018/08/13/revised PY - 2018/08/14/accepted PY - 2018/9/2/pubmed PY - 2018/12/12/medline PY - 2018/9/2/entrez KW - Adaptation KW - Coral KW - Microplastic KW - Stress KW - Symbiosis SP - 66 EP - 74 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ. Pollut. VL - 243 IS - Pt A N2 - Microplastics are widespread emerging contaminants that have been found globally in the marine and freshwater ecosystem, but there is limited knowledge regarding its impact on coral reef ecosystem and underpinning mechanism. In the present study, using Pocillopora damicornis as a model, we investigated cytological, physiological, and molecular responses of a scleractinian coral to acute microplastic exposure. No significant changes were observed in the density of symbiotic zooxanthellae during the entire period of microplastic exposure, while its chlorophyll content increased significantly at 12 h of microplastic exposure. We observed significant increases in the activities of antioxidant enzymes such as superoxide dismutase and catalase, significant decrease in the detoxifying enzyme glutathione S-transferase and the immune enzyme alkaline phosphatase, but no change in the other immune enzyme phenoloxidase during the whole experiment period. Transcriptomic analysis revealed 134 significantly up-regulated coral genes at 12 h after the exposure, enriched in 11 GO terms mostly related to stress response, zymogen granule, and JNK signal pathway. Meanwhile, 215 coral genes were significantly down-regulated at 12 h after exposure, enriched in 25 GO terms involved in sterol transport and EGF-ERK1/2 signal pathway. In contrast, only 12 zooxanthella genes exhibited significant up-regulation and 95 genes down-regulation at 12 h after the microplastic exposure; genes regulating synthesis and export of glucose and amino acids were not impacted. These results suggest that acute exposure of microplastics can activate the stress response of the scleractinian coral P. damicornis, and repress its detoxification and immune system through the JNK and ERK signal pathways. These demonstrate that microplastic exposure can compromise the anti-stress capacity and immune system of the scleractinian coral P. damicornis, despite the minimal impact on the abundance and major photosynthate translocation transporters of the symbiont in the short term. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/30172125/Acute_microplastic_exposure_raises_stress_response_and_suppresses_detoxification_and_immune_capacities_in_the_scleractinian_coral_Pocillopora_damicornis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0269-7491(18)32114-6 DB - PRIME DP - Unbound Medicine ER -