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Simultaneous HPLC determination of alfuzosin, tamsulosin and vardenafil in human plasma and pharmaceutical formulations using time programmed fluorescence detection.
Ann Pharm Fr. 2019 Jan; 77(1):28-37.AP

Abstract

Alfuzosin and tamsulosin are recently co-administrated with vardenafil to treat symptoms of benign prostatic hyperplasia and erectile dysfunction. A highly sensitive and simple liquid chromatographic method was developed and validated for the simultaneous determination of the three drugs using moxifloxacin as an internal standard. Isocratic separation was achieved within 7.0 min using phenyl-hexyl column (250 × 4.6 mm i.d.) and a mobile phase composed of acetonitrile/0.25% phosphoric acid (30:70, v/v) at pH 3.0. The analysis was performed at a flow rate of 1.2 mL/min with fluorescence detection at 246/450 nm for Alfuzosin and vardenafil, and 226/322nm for tamsulosin using time programming technique. The proposed method was linear over the concentration ranges of 5.0-50.0ng/mL, 10.0-200.0ng/mL and 20.0-400.0ng/mL for alfuzosin, vardenafil and tamsulosin, with limits of detection of 0.56ng/mL, 0.98ng/mL and 2.81 ng/mL in a respective order. The developed method was successfully applied to determine the studied drugs in dosage forms and human plasma samples and the results were satisfactory as revealed by statistical analysis of the data.

Authors+Show Affiliations

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516 Mansoura, Egypt.Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516 Mansoura, Egypt.Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516 Mansoura, Egypt.Unit of Drug Analysis, Faculty of Pharmacy, University of Mansoura, 35516 Mansoura, Egypt.Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Delta University for Science and Technology, International Coastal Road, Gamasa City, Dakahliya, Egypt. Electronic address: Hadeerborg@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30172351

Citation

Walash, M I., et al. "Simultaneous HPLC Determination of Alfuzosin, Tamsulosin and Vardenafil in Human Plasma and Pharmaceutical Formulations Using Time Programmed Fluorescence Detection." Annales Pharmaceutiques Francaises, vol. 77, no. 1, 2019, pp. 28-37.
Walash MI, Belal F, Fathy M, et al. Simultaneous HPLC determination of alfuzosin, tamsulosin and vardenafil in human plasma and pharmaceutical formulations using time programmed fluorescence detection. Ann Pharm Fr. 2019;77(1):28-37.
Walash, M. I., Belal, F., Fathy, M., Zayed, S., & Borg, H. (2019). Simultaneous HPLC determination of alfuzosin, tamsulosin and vardenafil in human plasma and pharmaceutical formulations using time programmed fluorescence detection. Annales Pharmaceutiques Francaises, 77(1), 28-37. https://doi.org/10.1016/j.pharma.2018.08.003
Walash MI, et al. Simultaneous HPLC Determination of Alfuzosin, Tamsulosin and Vardenafil in Human Plasma and Pharmaceutical Formulations Using Time Programmed Fluorescence Detection. Ann Pharm Fr. 2019;77(1):28-37. PubMed PMID: 30172351.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous HPLC determination of alfuzosin, tamsulosin and vardenafil in human plasma and pharmaceutical formulations using time programmed fluorescence detection. AU - Walash,M I, AU - Belal,F, AU - Fathy,M, AU - Zayed,S, AU - Borg,H, Y1 - 2018/08/29/ PY - 2018/03/05/received PY - 2018/08/02/revised PY - 2018/08/07/accepted PY - 2018/9/3/pubmed PY - 2019/4/23/medline PY - 2018/9/3/entrez KW - Alfuzosin KW - Alfuzosine KW - Détection de fluorescence KW - Fluorescence detection KW - Human plasma KW - Plasma humain KW - Programmation temporelle KW - Tamsulosin KW - Tamsulosine KW - Time programming KW - Vardenafil KW - Vardénafil SP - 28 EP - 37 JF - Annales pharmaceutiques francaises JO - Ann Pharm Fr VL - 77 IS - 1 N2 - Alfuzosin and tamsulosin are recently co-administrated with vardenafil to treat symptoms of benign prostatic hyperplasia and erectile dysfunction. A highly sensitive and simple liquid chromatographic method was developed and validated for the simultaneous determination of the three drugs using moxifloxacin as an internal standard. Isocratic separation was achieved within 7.0 min using phenyl-hexyl column (250 × 4.6 mm i.d.) and a mobile phase composed of acetonitrile/0.25% phosphoric acid (30:70, v/v) at pH 3.0. The analysis was performed at a flow rate of 1.2 mL/min with fluorescence detection at 246/450 nm for Alfuzosin and vardenafil, and 226/322nm for tamsulosin using time programming technique. The proposed method was linear over the concentration ranges of 5.0-50.0ng/mL, 10.0-200.0ng/mL and 20.0-400.0ng/mL for alfuzosin, vardenafil and tamsulosin, with limits of detection of 0.56ng/mL, 0.98ng/mL and 2.81 ng/mL in a respective order. The developed method was successfully applied to determine the studied drugs in dosage forms and human plasma samples and the results were satisfactory as revealed by statistical analysis of the data. SN - 0003-4509 UR - https://www.unboundmedicine.com/medline/citation/30172351/Simultaneous_HPLC_determination_of_alfuzosin,_tamsulosin_and_vardenafil_in_human_plasma_and_pharmaceutical_formulations_using_time_programmed_fluorescence_detection L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-4509(18)30037-3 DB - PRIME DP - Unbound Medicine ER -