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Development and application of degradation kinetics of thin layer chromatographic densitometry and conductometric methods for butoconazole nitrate determination.
Ann Pharm Fr 2018; 76(6):453-463AP

Abstract

Two simple methods were developed for determination of butoconazole nitrate (BN). The first developed method was stability-indicating HPTLC-densitometric method (method A) which is based on the quantitative densitometric separation of butoconazole nitrate (BN) from its degradation products on silica gel 60 F254 and measurement of the bands at 290nm. The developed stability study of BN was performed under different stress conditions including oxidative, hydrolytic, thermal and photolytic. Degradation was observed under acidic hydrolytic and oxidative conditions. Moreover, the HPTLC method was used to study the kinetics of BN acid degradation, determining as first order kinetics. The degradation rate constant of BN was found to be 0.076 hr-1 and t 1/2 value was determined at 9.12 hr in acidic medium. The second method (Method B) was conductometric method which is based on the reaction of BN with phosphotungstic acid (PTA) to form an ion associate in 50% methanol-water system. Validation of the proposed methods was carried out. All proposed methods were successfully applied for the commercial dosage form of BN. Statistical analysis of the results has been carried out revealing high accuracy and good precision.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig El Gamaa Street, 44519 Sharkia, Egypt. Electronic address: raniaadelsayed@gmail.com.Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig El Gamaa Street, 44519 Sharkia, Egypt.Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig El Gamaa Street, 44519 Sharkia, Egypt.Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig El Gamaa Street, 44519 Sharkia, Egypt.Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig El Gamaa Street, 44519 Sharkia, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30172352

Citation

Sayed, R A., et al. "Development and Application of Degradation Kinetics of Thin Layer Chromatographic Densitometry and Conductometric Methods for Butoconazole Nitrate Determination." Annales Pharmaceutiques Francaises, vol. 76, no. 6, 2018, pp. 453-463.
Sayed RA, Elmasry MS, Hassan WS, et al. Development and application of degradation kinetics of thin layer chromatographic densitometry and conductometric methods for butoconazole nitrate determination. Ann Pharm Fr. 2018;76(6):453-463.
Sayed, R. A., Elmasry, M. S., Hassan, W. S., El-Mammli, M. Y., & Shalaby, A. (2018). Development and application of degradation kinetics of thin layer chromatographic densitometry and conductometric methods for butoconazole nitrate determination. Annales Pharmaceutiques Francaises, 76(6), pp. 453-463. doi:10.1016/j.pharma.2018.07.007.
Sayed RA, et al. Development and Application of Degradation Kinetics of Thin Layer Chromatographic Densitometry and Conductometric Methods for Butoconazole Nitrate Determination. Ann Pharm Fr. 2018;76(6):453-463. PubMed PMID: 30172352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and application of degradation kinetics of thin layer chromatographic densitometry and conductometric methods for butoconazole nitrate determination. AU - Sayed,R A, AU - Elmasry,M S, AU - Hassan,W S, AU - El-Mammli,M Y, AU - Shalaby,A, Y1 - 2018/08/29/ PY - 2018/04/28/received PY - 2018/07/11/revised PY - 2018/07/23/accepted PY - 2018/9/3/pubmed PY - 2019/4/4/medline PY - 2018/9/3/entrez KW - Butoconazole nitrate KW - Conductometric methods KW - HPTLC-densitometry KW - HPTLC-densitométrie KW - Kinetic study KW - Method d’indication de stabilité KW - Methods conductimétriques KW - Stability indicating method KW - Étude cinétique SP - 453 EP - 463 JF - Annales pharmaceutiques francaises JO - Ann Pharm Fr VL - 76 IS - 6 N2 - Two simple methods were developed for determination of butoconazole nitrate (BN). The first developed method was stability-indicating HPTLC-densitometric method (method A) which is based on the quantitative densitometric separation of butoconazole nitrate (BN) from its degradation products on silica gel 60 F254 and measurement of the bands at 290nm. The developed stability study of BN was performed under different stress conditions including oxidative, hydrolytic, thermal and photolytic. Degradation was observed under acidic hydrolytic and oxidative conditions. Moreover, the HPTLC method was used to study the kinetics of BN acid degradation, determining as first order kinetics. The degradation rate constant of BN was found to be 0.076 hr-1 and t 1/2 value was determined at 9.12 hr in acidic medium. The second method (Method B) was conductometric method which is based on the reaction of BN with phosphotungstic acid (PTA) to form an ion associate in 50% methanol-water system. Validation of the proposed methods was carried out. All proposed methods were successfully applied for the commercial dosage form of BN. Statistical analysis of the results has been carried out revealing high accuracy and good precision. SN - 0003-4509 UR - https://www.unboundmedicine.com/medline/citation/30172352/Development_and_application_of_degradation_kinetics_of_thin_layer_chromatographic_densitometry_and_conductometric_methods_for_butoconazole_nitrate_determination L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-4509(18)30076-2 DB - PRIME DP - Unbound Medicine ER -