Tags

Type your tag names separated by a space and hit enter

Macular carotenoids in lipid food matrices: DOE-based high energy extraction of egg yolk xanthophylls and quantification through a validated APCI(+) LC-MS/MS method.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Oct 01; 1096:160-171.JC

Abstract

Lutein and zeaxanthin exhibit significant biological activities therefore their dietary intake through carotenoid-rich foods and supplements is strongly recommended as preventive approach. Hence their extraction from natural substrates targets to their commercial exploitation as nutraceuticals and ocular pharmaceuticals. Since carotenoids' bioavailability is higher in fat-containing substrates, egg yolk is considered an ideal food matrix. DOE-based optimization of novel high energy extraction practices achieves efficient recovery of xanthophylls from lipid sources. In this research, 23 full factorial and Box-Behnken designs (BBD) were applied for optimizing ultrasound- (UAE) and microwave-assisted extraction (MAE) variables (i.e. extraction solvent, temperature, time, US or MW power and solvent/material ratio). LC-MS/MS results pointed out the precedence of UAE in lutein and zeaxanthin extraction, where higher yields were obtained with 1:1 n-hexane-acetone as solvent mixture at 19 min, 600 W and 35 mL g-1. UAE carotenoid content was higher than MAE due to the different mechanisms laying behind the two processes and due to more complete granule rupture caused by higher US power. Evaluating the current results, DOE-based UAE analytical methodology stands out as an auspicious and sustainable alternative for commercial-based extraction of lipidic bioactive compounds for food and drug industrial applications.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Tsiaka T
Institute of Biology, Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, 48, Vas. Constantinou Ave., 11635 Athens, Greece; Laboratory of Chemistry, Analysis & Design of Food Processes, Department of Food Science and Technology, University of West Attica, Ag. Spyridonos, 1220 Egaleo, Greece; Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece.
Lantzouraki DZ
Institute of Biology, Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, 48, Vas. Constantinou Ave., 11635 Athens, Greece; Laboratory of Chemistry, Analysis & Design of Food Processes, Department of Food Science and Technology, University of West Attica, Ag. Spyridonos, 1220 Egaleo, Greece; Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece.
Siapi E
Institute of Biology, Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, 48, Vas. Constantinou Ave., 11635 Athens, Greece.
Sinanoglou VJ
Laboratory of Chemistry, Analysis & Design of Food Processes, Department of Food Science and Technology, University of West Attica, Ag. Spyridonos, 1220 Egaleo, Greece. Electronic address: vsina@teiath.gr.
Heropoulos GA
Institute of Biology, Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, 48, Vas. Constantinou Ave., 11635 Athens, Greece.
Calokerinos AC
Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece.
Zoumpoulakis P
Institute of Biology, Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, 48, Vas. Constantinou Ave., 11635 Athens, Greece. Electronic address: pzoump@eie.gr.

MeSH

Chromatography, LiquidEgg YolkLimit of DetectionLinear ModelsMicrowavesReproducibility of ResultsTandem Mass SpectrometryXanthophylls

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30173082

Citation

Tsiaka, Thalia, et al. "Macular Carotenoids in Lipid Food Matrices: DOE-based High Energy Extraction of Egg Yolk Xanthophylls and Quantification Through a Validated APCI(+) LC-MS/MS Method." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 1096, 2018, pp. 160-171.
Tsiaka T, Lantzouraki DZ, Siapi E, et al. Macular carotenoids in lipid food matrices: DOE-based high energy extraction of egg yolk xanthophylls and quantification through a validated APCI(+) LC-MS/MS method. J Chromatogr B Analyt Technol Biomed Life Sci. 2018;1096:160-171.
Tsiaka, T., Lantzouraki, D. Z., Siapi, E., Sinanoglou, V. J., Heropoulos, G. A., Calokerinos, A. C., & Zoumpoulakis, P. (2018). Macular carotenoids in lipid food matrices: DOE-based high energy extraction of egg yolk xanthophylls and quantification through a validated APCI(+) LC-MS/MS method. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1096, 160-171. https://doi.org/10.1016/j.jchromb.2018.08.010
Tsiaka T, et al. Macular Carotenoids in Lipid Food Matrices: DOE-based High Energy Extraction of Egg Yolk Xanthophylls and Quantification Through a Validated APCI(+) LC-MS/MS Method. J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Oct 1;1096:160-171. PubMed PMID: 30173082.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Macular carotenoids in lipid food matrices: DOE-based high energy extraction of egg yolk xanthophylls and quantification through a validated APCI(+) LC-MS/MS method. AU - Tsiaka,Thalia, AU - Lantzouraki,Dimitra Z, AU - Siapi,Eleni, AU - Sinanoglou,Vassilia J, AU - Heropoulos,Georgios A, AU - Calokerinos,Anthony C, AU - Zoumpoulakis,Panagiotis, Y1 - 2018/08/20/ PY - 2018/06/12/received PY - 2018/08/03/revised PY - 2018/08/16/accepted PY - 2018/9/3/pubmed PY - 2018/10/23/medline PY - 2018/9/3/entrez KW - Carotenoids KW - Egg yolk KW - Experimental design (DOE) KW - Microwave-assisted extraction (MAE) KW - Tandem liquid chromatography-mass spectrometry (LC-MS/MS) KW - Ultrasound-assisted extraction (UAE) SP - 160 EP - 171 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 1096 N2 - Lutein and zeaxanthin exhibit significant biological activities therefore their dietary intake through carotenoid-rich foods and supplements is strongly recommended as preventive approach. Hence their extraction from natural substrates targets to their commercial exploitation as nutraceuticals and ocular pharmaceuticals. Since carotenoids' bioavailability is higher in fat-containing substrates, egg yolk is considered an ideal food matrix. DOE-based optimization of novel high energy extraction practices achieves efficient recovery of xanthophylls from lipid sources. In this research, 23 full factorial and Box-Behnken designs (BBD) were applied for optimizing ultrasound- (UAE) and microwave-assisted extraction (MAE) variables (i.e. extraction solvent, temperature, time, US or MW power and solvent/material ratio). LC-MS/MS results pointed out the precedence of UAE in lutein and zeaxanthin extraction, where higher yields were obtained with 1:1 n-hexane-acetone as solvent mixture at 19 min, 600 W and 35 mL g-1. UAE carotenoid content was higher than MAE due to the different mechanisms laying behind the two processes and due to more complete granule rupture caused by higher US power. Evaluating the current results, DOE-based UAE analytical methodology stands out as an auspicious and sustainable alternative for commercial-based extraction of lipidic bioactive compounds for food and drug industrial applications. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/30173082/Macular_carotenoids_in_lipid_food_matrices:_DOE_based_high_energy_extraction_of_egg_yolk_xanthophylls_and_quantification_through_a_validated_APCI_+__LC_MS/MS_method_ DB - PRIME DP - Unbound Medicine ER -