Tags

Type your tag names separated by a space and hit enter

Effects of Lactococcus lactis subsp. lactis I2 with β-Glucooligosaccharides on Growth, Innate Immunity and Streptococcosis Resistance in Olive Flounder (Paralichthys olivaceus).
J Microbiol Biotechnol. 2018 Sep 28; 28(9):1433-1442.JM

Abstract

To identify and quantify the effects of a combination of dietary 1 × 108 CFU/g Lactococcus lactis subsp. lactis I2 (LI2) and 0.1% β-glucooligosaccharides (BGO) on the growth and immunity of olive flounder (Paralichthys olivaceus), a feeding experiment was conducted. Flounder (14 ± 0.5 g) were divided into two groups and fed control and synbiotic feeds for 8 weeks. Investigations were carried out on growth and feed utilization, innate immunity, serum biochemical parameters, intestinal lactic acid bacterial (LAB) viability, microvillus length, and changes in the expression levels of genes encoding pro-inflammatory cytokines (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, and IL-6). Results demonstrated the synbiotic diet had significantly better (p < 0.05) responses in terms of weight gain and specific growth rate, three innate immune parameters (respiratory burst, serum lysozyme, and superoxide dismutase), intestinal LAB viability, and the relative TNF-α expression level (p < 0.05). Moreover, after challenge with Streptococcus iniae (1 × 108 CFU/ml), the synbiotically fed group exhibited significantly higher (p < 0.05) protection against streptococcosis, validating the observed changes in immune parameters and induction of the cytokine-encoding gene. Therefore, according to the results of the present study, synbiotic feed (LI2 + BGO) increased growth, modulated innate immune parameters and protected olive flounder against streptococcosis.

Authors+Show Affiliations

Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.Aquafeed Research Center, NIFS, Pohang 46083, Republic of Korea.Aquafeed Research Center, NIFS, Pohang 46083, Republic of Korea.Aquafeed Research Center, NIFS, Pohang 46083, Republic of Korea.Aquafeed Research Center, NIFS, Pohang 46083, Republic of Korea.Department of Aquatic Life Medicine, Pukyong National University, Busan 48513, Republic of Korea.Department of Aquatic Life Medicine, Pukyong National University, Busan 48513, Republic of Korea.Aquaculture Research Division, NIFS, Busan 46083, Republic of Korea.Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30176713

Citation

Hasan, Md Tawheed, et al. "Effects of Lactococcus Lactis Subsp. Lactis I2 With β-Glucooligosaccharides On Growth, Innate Immunity and Streptococcosis Resistance in Olive Flounder (Paralichthys Olivaceus)." Journal of Microbiology and Biotechnology, vol. 28, no. 9, 2018, pp. 1433-1442.
Hasan MT, Jang WJ, Tak JY, et al. Effects of Lactococcus lactis subsp. lactis I2 with β-Glucooligosaccharides on Growth, Innate Immunity and Streptococcosis Resistance in Olive Flounder (Paralichthys olivaceus). J Microbiol Biotechnol. 2018;28(9):1433-1442.
Hasan, M. T., Jang, W. J., Tak, J. Y., Lee, B. J., Kim, K. W., Hur, S. W., Han, H. S., Kim, B. S., Min, D. H., Kim, S. K., & Kong, I. S. (2018). Effects of Lactococcus lactis subsp. lactis I2 with β-Glucooligosaccharides on Growth, Innate Immunity and Streptococcosis Resistance in Olive Flounder (Paralichthys olivaceus). Journal of Microbiology and Biotechnology, 28(9), 1433-1442. https://doi.org/10.4014/jmb.1805.05011
Hasan MT, et al. Effects of Lactococcus Lactis Subsp. Lactis I2 With β-Glucooligosaccharides On Growth, Innate Immunity and Streptococcosis Resistance in Olive Flounder (Paralichthys Olivaceus). J Microbiol Biotechnol. 2018 Sep 28;28(9):1433-1442. PubMed PMID: 30176713.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Lactococcus lactis subsp. lactis I2 with β-Glucooligosaccharides on Growth, Innate Immunity and Streptococcosis Resistance in Olive Flounder (Paralichthys olivaceus). AU - Hasan,Md Tawheed, AU - Jang,Won Je, AU - Tak,Jin Yeong, AU - Lee,Bong-Joo, AU - Kim,Kang Woong, AU - Hur,Sang Woo, AU - Han,Hyon-Sob, AU - Kim,Bo-Seong, AU - Min,Do-Huh, AU - Kim,Shin-Kwon, AU - Kong,In-Soo, PY - 2018/9/5/pubmed PY - 2019/5/1/medline PY - 2018/9/5/entrez KW - Synbiotic KW - gene expression KW - growth performance KW - innate immunity KW - olive flounder SP - 1433 EP - 1442 JF - Journal of microbiology and biotechnology JO - J Microbiol Biotechnol VL - 28 IS - 9 N2 - To identify and quantify the effects of a combination of dietary 1 × 108 CFU/g Lactococcus lactis subsp. lactis I2 (LI2) and 0.1% β-glucooligosaccharides (BGO) on the growth and immunity of olive flounder (Paralichthys olivaceus), a feeding experiment was conducted. Flounder (14 ± 0.5 g) were divided into two groups and fed control and synbiotic feeds for 8 weeks. Investigations were carried out on growth and feed utilization, innate immunity, serum biochemical parameters, intestinal lactic acid bacterial (LAB) viability, microvillus length, and changes in the expression levels of genes encoding pro-inflammatory cytokines (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, and IL-6). Results demonstrated the synbiotic diet had significantly better (p < 0.05) responses in terms of weight gain and specific growth rate, three innate immune parameters (respiratory burst, serum lysozyme, and superoxide dismutase), intestinal LAB viability, and the relative TNF-α expression level (p < 0.05). Moreover, after challenge with Streptococcus iniae (1 × 108 CFU/ml), the synbiotically fed group exhibited significantly higher (p < 0.05) protection against streptococcosis, validating the observed changes in immune parameters and induction of the cytokine-encoding gene. Therefore, according to the results of the present study, synbiotic feed (LI2 + BGO) increased growth, modulated innate immune parameters and protected olive flounder against streptococcosis. SN - 1738-8872 UR - https://www.unboundmedicine.com/medline/citation/30176713/Effects_of_Lactococcus_lactis_subsp__lactis_I2_with_β_Glucooligosaccharides_on_Growth_Innate_Immunity_and_Streptococcosis_Resistance_in_Olive_Flounder__Paralichthys_olivaceus__ L2 - http://www.jmb.or.kr/journal/view.html?doi=10.4014/jmb.1805.05011 DB - PRIME DP - Unbound Medicine ER -