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Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry.
Biomed Res Int 2018; 2018:1578643BR

Abstract

Background and Aims

The present study aimed to develop a simple and sensitive method for quantitative determination of monocrotaline (MCT) in mouse blood employing ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI/MS/MS) using rhynchophylline as an internal standard.

Methods

Proteins present in the blood samples were precipitated using acetonitrile. MCT was separated using a 1.7-μm ethylene bridged hybrid (BEH) C18 column (2.1 mm × 50 mm) with a gradient elution program and a constant flow rate of 0.4 mL/min. The LC mobile phase consisted of 10 mmol/L ammonium acetate (containing 0.1% formic acid) and acetonitrile. The total elution time was 4.0 min. The analytes were detected on a UPLC-ESI mass spectrometer in multiple reaction monitoring (MRM) mode and quantified.

Results

The new method for the determination of MCT has a satisfactory linear detection range of 1-2000 ng/mL and excellent linearity (r = 0.9971). The lower limit of quantification (LLOQ) of MCT is 1.0 ng/mL. Intra- and interassay precisions of MCT were ≤13% with an accuracy from 96.2% to 106.6%. The average recovery of the new method was >75.0%, and matrix effects were between 89.0% and 94.3%. Based on the pharmacokinetics data, the bioavailability of MCT in mice was 88.3% after oral administration.

Conclusions

The results suggest that the newly standardized method for quantitative determination of MCT in whole blood is fast, reliable, specific, sensitive, and suitable for pharmacokinetic studies of MCT after intravenous or intragastric administration.

Authors+Show Affiliations

The Third Clinical Institute Affiliated with Wenzhou Medical University & Wenzhou People's Hospital, Wenzhou 325000, China.Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.The Third Clinical Institute Affiliated with Wenzhou Medical University & Wenzhou People's Hospital, Wenzhou 325000, China.Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.Laboratory of Clinical Pharmacy, The People's Hospital of Lishui, Lishui 323000, China.Analytical and Testing Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China.The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30186850

Citation

Chen, Lianguo, et al. "Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood By Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry." BioMed Research International, vol. 2018, 2018, p. 1578643.
Chen L, Zhang B, Liu J, et al. Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. Biomed Res Int. 2018;2018:1578643.
Chen, L., Zhang, B., Liu, J., Fan, Z., Weng, Z., Geng, P., ... Lin, G. (2018). Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. BioMed Research International, 2018, p. 1578643. doi:10.1155/2018/1578643.
Chen L, et al. Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood By Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. Biomed Res Int. 2018;2018:1578643. PubMed PMID: 30186850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry. AU - Chen,Lianguo, AU - Zhang,Bin, AU - Liu,Jinlai, AU - Fan,Zhehua, AU - Weng,Ziwei, AU - Geng,Peiwu, AU - Wang,Xianqin, AU - Lin,Guanyang, Y1 - 2018/08/13/ PY - 2018/04/11/received PY - 2018/07/05/revised PY - 2018/07/29/accepted PY - 2018/9/7/entrez PY - 2018/9/7/pubmed PY - 2018/12/12/medline SP - 1578643 EP - 1578643 JF - BioMed research international JO - Biomed Res Int VL - 2018 N2 - Background and Aims: The present study aimed to develop a simple and sensitive method for quantitative determination of monocrotaline (MCT) in mouse blood employing ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI/MS/MS) using rhynchophylline as an internal standard. Methods: Proteins present in the blood samples were precipitated using acetonitrile. MCT was separated using a 1.7-μm ethylene bridged hybrid (BEH) C18 column (2.1 mm × 50 mm) with a gradient elution program and a constant flow rate of 0.4 mL/min. The LC mobile phase consisted of 10 mmol/L ammonium acetate (containing 0.1% formic acid) and acetonitrile. The total elution time was 4.0 min. The analytes were detected on a UPLC-ESI mass spectrometer in multiple reaction monitoring (MRM) mode and quantified. Results: The new method for the determination of MCT has a satisfactory linear detection range of 1-2000 ng/mL and excellent linearity (r = 0.9971). The lower limit of quantification (LLOQ) of MCT is 1.0 ng/mL. Intra- and interassay precisions of MCT were ≤13% with an accuracy from 96.2% to 106.6%. The average recovery of the new method was >75.0%, and matrix effects were between 89.0% and 94.3%. Based on the pharmacokinetics data, the bioavailability of MCT in mice was 88.3% after oral administration. Conclusions: The results suggest that the newly standardized method for quantitative determination of MCT in whole blood is fast, reliable, specific, sensitive, and suitable for pharmacokinetic studies of MCT after intravenous or intragastric administration. SN - 2314-6141 UR - https://www.unboundmedicine.com/medline/citation/30186850/Pharmacokinetics_and_Bioavailability_Study_of_Monocrotaline_in_Mouse_Blood_by_Ultra_Performance_Liquid_Chromatography_Tandem_Mass_Spectrometry_ L2 - https://dx.doi.org/10.1155/2018/1578643 DB - PRIME DP - Unbound Medicine ER -