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Comprehensive characterisation of polyphenols in leaves and stems of three anti-dengue virus type-2 active Brazilian Faramea species (Rubiaceae) by HPLC-DAD-ESI-MS/MS.
Phytochem Anal. 2019 Jan; 30(1):62-72.PA

Abstract

INTRODUCTION

The methanol (MeOH) leaf extracts of the species Faramea bahiensis, F. hyacinthina and F. truncata (Rubiaceae) have previously shown in vitro non-cytotoxic and anti-dengue virus serotype 2 (DENV2) activities in human hepatocarcinoma cell lineage (HepG2). Chemical studies have led to the isolation of major flavonoids, but quite complex fractions of phenolic compounds still remain.

OBJECTIVE

To complete the study of phenolic compounds in the leaves and to access the presence of these compounds in the stems of these Faramea spp. by online high-performance liquid chromatography-diode array detector-electrospray ionisation tandem mass spectrometry (HPLC-DAD-ESI-MS/MS), as well as to evaluate the in vitro cytotoxic and anti-DENV2 activities of their MeOH stem extracts.

METHODOLOGY

The identification was performed by comparing retention times, UV and mass spectra with those of available standards and by using the mechanisms and fragmentation patterns established in previous studies. The effects of the extracts in DENV2 infected HepG2 cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The virus titer was quantified by plaque assay.

RESULTS

The study led to the characterisation of 31 phenolic compounds including flavonoid O- and C-glycosides, phenolic acids and one coumarin. The stem extracts from F. hyacinthina and F. bahiensis presented a similar bioactivity to those of their leaves but a loss of cytoprotective activity of F. bahiensis and a higher cytotoxicity of F. truncata were observed.

CONCLUSIONS

This research allowed a detailed phenolic composition of three bioactive Faramea species to be achieved, thus contributing to the study of this genus and providing valuable information for further phytotherapeutic applications.

Authors+Show Affiliations

Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), Bilbao, Spain.Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Instituto de Microbiologia Paulo Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Instituto de Microbiologia Paulo Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Instituto de Microbiologia Paulo Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rio de Janeiro, Brazil.Departamento de Química Analítica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), Bilbao, Spain.Departamento de Química Orgánica, Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), Bilbao, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30191624

Citation

Wolff, Thiago, et al. "Comprehensive Characterisation of Polyphenols in Leaves and Stems of Three Anti-dengue Virus Type-2 Active Brazilian Faramea Species (Rubiaceae) By HPLC-DAD-ESI-MS/MS." Phytochemical Analysis : PCA, vol. 30, no. 1, 2019, pp. 62-72.
Wolff T, Berrueta LA, Valente LMM, et al. Comprehensive characterisation of polyphenols in leaves and stems of three anti-dengue virus type-2 active Brazilian Faramea species (Rubiaceae) by HPLC-DAD-ESI-MS/MS. Phytochem Anal. 2019;30(1):62-72.
Wolff, T., Berrueta, L. A., Valente, L. M. M., Barboza, R. S., Neris, R. L. S., Guimarães-Andrade, I. P., Assunção-Miranda, I., Nascimento, A. C., Gomes, M., Gallo, B., & Iriondo, C. (2019). Comprehensive characterisation of polyphenols in leaves and stems of three anti-dengue virus type-2 active Brazilian Faramea species (Rubiaceae) by HPLC-DAD-ESI-MS/MS. Phytochemical Analysis : PCA, 30(1), 62-72. https://doi.org/10.1002/pca.2790
Wolff T, et al. Comprehensive Characterisation of Polyphenols in Leaves and Stems of Three Anti-dengue Virus Type-2 Active Brazilian Faramea Species (Rubiaceae) By HPLC-DAD-ESI-MS/MS. Phytochem Anal. 2019;30(1):62-72. PubMed PMID: 30191624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comprehensive characterisation of polyphenols in leaves and stems of three anti-dengue virus type-2 active Brazilian Faramea species (Rubiaceae) by HPLC-DAD-ESI-MS/MS. AU - Wolff,Thiago, AU - Berrueta,Luis A, AU - Valente,Ligia M M, AU - Barboza,Rodolfo S, AU - Neris,Rômulo L S, AU - Guimarães-Andrade,Iris P, AU - Assunção-Miranda,Iranaia, AU - Nascimento,Adriana C, AU - Gomes,Mário, AU - Gallo,Blanca, AU - Iriondo,Carmen, Y1 - 2018/09/07/ PY - 2018/02/21/received PY - 2018/06/22/revised PY - 2018/07/07/accepted PY - 2018/9/8/pubmed PY - 2019/2/1/medline PY - 2018/9/8/entrez KW - Faramea KW - HPLC-DAD-ESI-MS/MS KW - Rubiaceae KW - anti-dengue activity KW - dengue virus KW - polyphenols SP - 62 EP - 72 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 30 IS - 1 N2 - INTRODUCTION: The methanol (MeOH) leaf extracts of the species Faramea bahiensis, F. hyacinthina and F. truncata (Rubiaceae) have previously shown in vitro non-cytotoxic and anti-dengue virus serotype 2 (DENV2) activities in human hepatocarcinoma cell lineage (HepG2). Chemical studies have led to the isolation of major flavonoids, but quite complex fractions of phenolic compounds still remain. OBJECTIVE: To complete the study of phenolic compounds in the leaves and to access the presence of these compounds in the stems of these Faramea spp. by online high-performance liquid chromatography-diode array detector-electrospray ionisation tandem mass spectrometry (HPLC-DAD-ESI-MS/MS), as well as to evaluate the in vitro cytotoxic and anti-DENV2 activities of their MeOH stem extracts. METHODOLOGY: The identification was performed by comparing retention times, UV and mass spectra with those of available standards and by using the mechanisms and fragmentation patterns established in previous studies. The effects of the extracts in DENV2 infected HepG2 cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The virus titer was quantified by plaque assay. RESULTS: The study led to the characterisation of 31 phenolic compounds including flavonoid O- and C-glycosides, phenolic acids and one coumarin. The stem extracts from F. hyacinthina and F. bahiensis presented a similar bioactivity to those of their leaves but a loss of cytoprotective activity of F. bahiensis and a higher cytotoxicity of F. truncata were observed. CONCLUSIONS: This research allowed a detailed phenolic composition of three bioactive Faramea species to be achieved, thus contributing to the study of this genus and providing valuable information for further phytotherapeutic applications. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/30191624/Comprehensive_characterisation_of_polyphenols_in_leaves_and_stems_of_three_anti_dengue_virus_type_2_active_Brazilian_Faramea_species__Rubiaceae__by_HPLC_DAD_ESI_MS/MS_ L2 - https://doi.org/10.1002/pca.2790 DB - PRIME DP - Unbound Medicine ER -