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Aberrant Stamen Development is Associated with Parthenocarpic Fruit Set Through Up-Regulation of Gibberellin Biosynthesis in Tomato.
Plant Cell Physiol. 2019 Jan 01; 60(1):38-51.PC

Abstract

Parthenocarpy, a process in which fruit set occurs without fertilization, leads to the production of seedless fruit. A number of floral homeotic mutants with abnormal stamen development exhibit parthenocarpic fruit set. Flower development is thought to repress ovary growth before anthesis. However, the mechanism of parthenocarpic fruit development caused by aberrant flower formation is poorly understood. To investigate the molecular mechanism of parthenocarpic fruit development in floral homeotic mutants, we performed functional analysis of Tomato APETALA3 (TAP3) by loss-of-function approaches. Organ-specific promoter was used to induce organ-specific loss of function in stamen and ovary/fruit. We observed increased cell expansion in tap3 mutants and TAP3-RNAi lines during parthenocarpic fruit growth. These were predominantly accompanied by the up-regulation of GA biosynthesis genes, including SlGA20ox1, SlGA20ox2, and SlGA20ox3, as well as reduced expression of the GA-inactivating gene SlGA2ox1 and the auxin signaling gene SlARF7 involved in a crosstalk between GA and auxin. These transcriptional profiles are in agreement with the GA levels in these lines. These results suggest that stamen development negatively regulates fruit set by repressing the GA biosynthesis.

Authors+Show Affiliations

Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, Japan. Tsukuba Plant Innovation Research Center, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, Japan.Graduate School of Environmental and Life and Sciences, Okayama University, Tsushima, Okayama, Japan.Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, Japan. Tsukuba Plant Innovation Research Center, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, Japan.Graduate School of Environmental and Life and Sciences, Okayama University, Tsushima, Okayama, Japan.RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro, Tsurumi, Yokohama, Japan.RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro, Tsurumi, Yokohama, Japan.RIKEN Center for Sustainable Resource Science, 1-7-22, Suehiro, Tsurumi, Yokohama, Japan. Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan.Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, Japan. Tsukuba Plant Innovation Research Center, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, Japan.Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, Japan.Graduate School Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, Japan.Graduate School of Environmental and Life and Sciences, Okayama University, Tsushima, Okayama, Japan.Graduate School of Environmental and Life and Sciences, Okayama University, Tsushima, Okayama, Japan.Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, Japan. Tsukuba Plant Innovation Research Center, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30192961

Citation

Okabe, Yoshihiro, et al. "Aberrant Stamen Development Is Associated With Parthenocarpic Fruit Set Through Up-Regulation of Gibberellin Biosynthesis in Tomato." Plant & Cell Physiology, vol. 60, no. 1, 2019, pp. 38-51.
Okabe Y, Yamaoka T, Ariizumi T, et al. Aberrant Stamen Development is Associated with Parthenocarpic Fruit Set Through Up-Regulation of Gibberellin Biosynthesis in Tomato. Plant Cell Physiol. 2019;60(1):38-51.
Okabe, Y., Yamaoka, T., Ariizumi, T., Ushijima, K., Kojima, M., Takebayashi, Y., Sakakibara, H., Kusano, M., Shinozaki, Y., Pulungan, S. I., Kubo, Y., Nakano, R., & Ezura, H. (2019). Aberrant Stamen Development is Associated with Parthenocarpic Fruit Set Through Up-Regulation of Gibberellin Biosynthesis in Tomato. Plant & Cell Physiology, 60(1), 38-51. https://doi.org/10.1093/pcp/pcy184
Okabe Y, et al. Aberrant Stamen Development Is Associated With Parthenocarpic Fruit Set Through Up-Regulation of Gibberellin Biosynthesis in Tomato. Plant Cell Physiol. 2019 Jan 1;60(1):38-51. PubMed PMID: 30192961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aberrant Stamen Development is Associated with Parthenocarpic Fruit Set Through Up-Regulation of Gibberellin Biosynthesis in Tomato. AU - Okabe,Yoshihiro, AU - Yamaoka,Tatsuya, AU - Ariizumi,Tohru, AU - Ushijima,Koichiro, AU - Kojima,Mikiko, AU - Takebayashi,Yumiko, AU - Sakakibara,Hitoshi, AU - Kusano,Miyako, AU - Shinozaki,Yoshihito, AU - Pulungan,Sri Imriani, AU - Kubo,Yasutaka, AU - Nakano,Ryohei, AU - Ezura,Hiroshi, PY - 2018/02/23/received PY - 2018/09/04/accepted PY - 2018/9/8/pubmed PY - 2019/3/6/medline PY - 2018/9/8/entrez SP - 38 EP - 51 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 60 IS - 1 N2 - Parthenocarpy, a process in which fruit set occurs without fertilization, leads to the production of seedless fruit. A number of floral homeotic mutants with abnormal stamen development exhibit parthenocarpic fruit set. Flower development is thought to repress ovary growth before anthesis. However, the mechanism of parthenocarpic fruit development caused by aberrant flower formation is poorly understood. To investigate the molecular mechanism of parthenocarpic fruit development in floral homeotic mutants, we performed functional analysis of Tomato APETALA3 (TAP3) by loss-of-function approaches. Organ-specific promoter was used to induce organ-specific loss of function in stamen and ovary/fruit. We observed increased cell expansion in tap3 mutants and TAP3-RNAi lines during parthenocarpic fruit growth. These were predominantly accompanied by the up-regulation of GA biosynthesis genes, including SlGA20ox1, SlGA20ox2, and SlGA20ox3, as well as reduced expression of the GA-inactivating gene SlGA2ox1 and the auxin signaling gene SlARF7 involved in a crosstalk between GA and auxin. These transcriptional profiles are in agreement with the GA levels in these lines. These results suggest that stamen development negatively regulates fruit set by repressing the GA biosynthesis. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/30192961/Aberrant_Stamen_Development_is_Associated_with_Parthenocarpic_Fruit_Set_Through_Up_Regulation_of_Gibberellin_Biosynthesis_in_Tomato_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcy184 DB - PRIME DP - Unbound Medicine ER -