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The mechanism of action of a fat regulator: Glycyrrhetinic acid (GA) stimulating fatty acid transmembrane and intracellular transport in blunt snout bream (Megalobrama amblycephala).

Abstract

High-fat diets are associated with fatty liver and aberrant hepatic lipid metabolism, and glycyrrhetinic acid (GA) has been shown to exert a beneficial effect on lipolysis and fat deposition in fish. In the present study, we evaluated the effect of GA on the growth performance and expression of hepatic lipid transport related genes in blunt snout bream (Megalobrama amblycephala) fed a high fat diet. Two hundred and sixteen fish (average body weight: 45.57 g ± 0.98 g) were fed three experimental diets (6% fat/L6 group, control, 11% fat/L11 group, and 11% fat with 0.3 mg kg-1 GA/L11GA group) for 8 weeks. Compared to the control group, the weight gain and specific growth rate of high-fat fed group at the end of the trialwere significantly improved (P < .05).However, GA showed no effect on animals' growth performance(P > .05). Dietary supplementation with 0.3 mg kg-1 GA significantly decreased the hepatosomatic index, viscera/body ratio, and intraperitoneal fat ratio (P < .05), and up-regulated the expression levels of fatty acids transport protein (FATP), fatty acids binding protein (FABP), fatty acid translocase (CD36), carnitine palmitoyl transferase I (CPT1) and peroxisome proliferators-activated receptors α (PPARα) compared to both the L6 group and L11 group (P < .05). However, no significant difference was observed in fatty acid synthetase (FAS), acetyl-CoA carboxylase α (ACCα), or lipoprotein lipase (LPL) between groups (P > .05). In conclusion, GA significantly rescued high-fat diet induced hepatic lipid accumulation and metabolism dysfunction in M. amblycephalaby stimulating hepatic fatty acid transport and β-oxidation. Dietary GA may be used as a promising supplement to alleviate high-fat diet induced side effects on M. amblycephala.

Authors+Show Affiliations

Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing 210095, People's Republic of China.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing 210095, People's Republic of China.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing 210095, People's Republic of China.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing 210095, People's Republic of China.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, No.1 Weigang Road, Nanjing 210095, People's Republic of China. Electronic address: wbliu@njau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30193864

Citation

Jiang, Guang-Zhen, et al. "The Mechanism of Action of a Fat Regulator: Glycyrrhetinic Acid (GA) Stimulating Fatty Acid Transmembrane and Intracellular Transport in Blunt Snout Bream (Megalobrama Amblycephala)." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 226, 2018, pp. 83-90.
Jiang GZ, Zhou M, Zhang DD, et al. The mechanism of action of a fat regulator: Glycyrrhetinic acid (GA) stimulating fatty acid transmembrane and intracellular transport in blunt snout bream (Megalobrama amblycephala). Comp Biochem Physiol, Part A Mol Integr Physiol. 2018;226:83-90.
Jiang, G. Z., Zhou, M., Zhang, D. D., Li, X. F., & Liu, W. B. (2018). The mechanism of action of a fat regulator: Glycyrrhetinic acid (GA) stimulating fatty acid transmembrane and intracellular transport in blunt snout bream (Megalobrama amblycephala). Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 226, pp. 83-90. doi:10.1016/j.cbpa.2018.08.014.
Jiang GZ, et al. The Mechanism of Action of a Fat Regulator: Glycyrrhetinic Acid (GA) Stimulating Fatty Acid Transmembrane and Intracellular Transport in Blunt Snout Bream (Megalobrama Amblycephala). Comp Biochem Physiol, Part A Mol Integr Physiol. 2018;226:83-90. PubMed PMID: 30193864.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The mechanism of action of a fat regulator: Glycyrrhetinic acid (GA) stimulating fatty acid transmembrane and intracellular transport in blunt snout bream (Megalobrama amblycephala). AU - Jiang,Guang-Zhen, AU - Zhou,Man, AU - Zhang,Ding-Dong, AU - Li,Xiang-Fei, AU - Liu,Wen-Bin, Y1 - 2018/09/05/ PY - 2018/05/21/received PY - 2018/08/16/revised PY - 2018/08/27/accepted PY - 2018/9/9/pubmed PY - 2019/7/19/medline PY - 2018/9/9/entrez KW - Fatty acid transport KW - Glycyrrhetinic acid (GA) KW - High-fat diet KW - Megalobrama amblycephala SP - 83 EP - 90 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. VL - 226 N2 - High-fat diets are associated with fatty liver and aberrant hepatic lipid metabolism, and glycyrrhetinic acid (GA) has been shown to exert a beneficial effect on lipolysis and fat deposition in fish. In the present study, we evaluated the effect of GA on the growth performance and expression of hepatic lipid transport related genes in blunt snout bream (Megalobrama amblycephala) fed a high fat diet. Two hundred and sixteen fish (average body weight: 45.57 g ± 0.98 g) were fed three experimental diets (6% fat/L6 group, control, 11% fat/L11 group, and 11% fat with 0.3 mg kg-1 GA/L11GA group) for 8 weeks. Compared to the control group, the weight gain and specific growth rate of high-fat fed group at the end of the trialwere significantly improved (P < .05).However, GA showed no effect on animals' growth performance(P > .05). Dietary supplementation with 0.3 mg kg-1 GA significantly decreased the hepatosomatic index, viscera/body ratio, and intraperitoneal fat ratio (P < .05), and up-regulated the expression levels of fatty acids transport protein (FATP), fatty acids binding protein (FABP), fatty acid translocase (CD36), carnitine palmitoyl transferase I (CPT1) and peroxisome proliferators-activated receptors α (PPARα) compared to both the L6 group and L11 group (P < .05). However, no significant difference was observed in fatty acid synthetase (FAS), acetyl-CoA carboxylase α (ACCα), or lipoprotein lipase (LPL) between groups (P > .05). In conclusion, GA significantly rescued high-fat diet induced hepatic lipid accumulation and metabolism dysfunction in M. amblycephalaby stimulating hepatic fatty acid transport and β-oxidation. Dietary GA may be used as a promising supplement to alleviate high-fat diet induced side effects on M. amblycephala. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/30193864/The_mechanism_of_action_of_a_fat_regulator:_Glycyrrhetinic_acid__GA__stimulating_fatty_acid_transmembrane_and_intracellular_transport_in_blunt_snout_bream__Megalobrama_amblycephala__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(18)30141-7 DB - PRIME DP - Unbound Medicine ER -