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Engineering Trichoderma reesei Rut-C30 with the overexpression of egl1 at the ace1 locus to relieve repression on cellulase production and to adjust the ratio of cellulolytic enzymes for more efficient hydrolysis of lignocellulosic biomass.
J Biotechnol. 2018 Nov 10; 285:56-63.JB

Abstract

Cellulose hydrolysis is a synergetic process performed sequentially by different cellulolytic enzymes including endoglucanases, exoglucanases and glucosidases. Trichoderma reesei has been acknowledged as the best cellulase producer, but cellulase production by T. reesei through submerged fermentation is costly due to intensive energy consumption associated with the process for mixing and aeration, since non-Newtonian fluid properties are developed with mycelial growth. Therefore, engineering the ratio of cellulolytic enzymes in the cocktail for more efficient cellulose hydrolysis is an alternative strategy for reducing cellulase dosage and thus saving cost in enzyme consumption for cellulose hydrolysis. In this study, T. reesei QS305 with high endoglucanase activity was developed from T. reesei Rut-C30 by replacing the transcription repressor gene ace1 with the coding region of endoglucanase gene egl1. Compared to T. reesei Rut-C30, T. reesei QS305 showed 90.0% and 132.7% increase in the activities of total cellulases and endoglucanases under flask culture conditions. When cellulase production by T. reesei QS305 was performed in the 5-L fermentor, cellulases activity of 10.7 FPU/mL was achieved at 108 h, 75.4% higher than that produced by T. reesei Rut-C30. Moreover, cellulases produced by T. reesei QS305 were more efficient for hydrolyzing pretreated corn stover and Jerusalem artichoke stalk.

Authors+Show Affiliations

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China.State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: xqzhao@sjtu.edu.cn.School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China; State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: fwbai@sjtu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30194052

Citation

Meng, Qing-Shan, et al. "Engineering Trichoderma Reesei Rut-C30 With the Overexpression of Egl1 at the Ace1 Locus to Relieve Repression On Cellulase Production and to Adjust the Ratio of Cellulolytic Enzymes for More Efficient Hydrolysis of Lignocellulosic Biomass." Journal of Biotechnology, vol. 285, 2018, pp. 56-63.
Meng QS, Liu CG, Zhao XQ, et al. Engineering Trichoderma reesei Rut-C30 with the overexpression of egl1 at the ace1 locus to relieve repression on cellulase production and to adjust the ratio of cellulolytic enzymes for more efficient hydrolysis of lignocellulosic biomass. J Biotechnol. 2018;285:56-63.
Meng, Q. S., Liu, C. G., Zhao, X. Q., & Bai, F. W. (2018). Engineering Trichoderma reesei Rut-C30 with the overexpression of egl1 at the ace1 locus to relieve repression on cellulase production and to adjust the ratio of cellulolytic enzymes for more efficient hydrolysis of lignocellulosic biomass. Journal of Biotechnology, 285, 56-63. https://doi.org/10.1016/j.jbiotec.2018.09.001
Meng QS, et al. Engineering Trichoderma Reesei Rut-C30 With the Overexpression of Egl1 at the Ace1 Locus to Relieve Repression On Cellulase Production and to Adjust the Ratio of Cellulolytic Enzymes for More Efficient Hydrolysis of Lignocellulosic Biomass. J Biotechnol. 2018 Nov 10;285:56-63. PubMed PMID: 30194052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Engineering Trichoderma reesei Rut-C30 with the overexpression of egl1 at the ace1 locus to relieve repression on cellulase production and to adjust the ratio of cellulolytic enzymes for more efficient hydrolysis of lignocellulosic biomass. AU - Meng,Qing-Shan, AU - Liu,Chen-Guang, AU - Zhao,Xin-Qing, AU - Bai,Feng-Wu, Y1 - 2018/09/05/ PY - 2018/06/11/received PY - 2018/08/06/revised PY - 2018/09/03/accepted PY - 2018/9/9/pubmed PY - 2018/12/12/medline PY - 2018/9/9/entrez KW - Cellulases KW - Endoglucanases KW - Transcription repressor ACE1 KW - Trichoderma reesei SP - 56 EP - 63 JF - Journal of biotechnology JO - J. Biotechnol. VL - 285 N2 - Cellulose hydrolysis is a synergetic process performed sequentially by different cellulolytic enzymes including endoglucanases, exoglucanases and glucosidases. Trichoderma reesei has been acknowledged as the best cellulase producer, but cellulase production by T. reesei through submerged fermentation is costly due to intensive energy consumption associated with the process for mixing and aeration, since non-Newtonian fluid properties are developed with mycelial growth. Therefore, engineering the ratio of cellulolytic enzymes in the cocktail for more efficient cellulose hydrolysis is an alternative strategy for reducing cellulase dosage and thus saving cost in enzyme consumption for cellulose hydrolysis. In this study, T. reesei QS305 with high endoglucanase activity was developed from T. reesei Rut-C30 by replacing the transcription repressor gene ace1 with the coding region of endoglucanase gene egl1. Compared to T. reesei Rut-C30, T. reesei QS305 showed 90.0% and 132.7% increase in the activities of total cellulases and endoglucanases under flask culture conditions. When cellulase production by T. reesei QS305 was performed in the 5-L fermentor, cellulases activity of 10.7 FPU/mL was achieved at 108 h, 75.4% higher than that produced by T. reesei Rut-C30. Moreover, cellulases produced by T. reesei QS305 were more efficient for hydrolyzing pretreated corn stover and Jerusalem artichoke stalk. SN - 1873-4863 UR - https://www.unboundmedicine.com/medline/citation/30194052/Engineering_Trichoderma_reesei_Rut_C30_with_the_overexpression_of_egl1_at_the_ace1_locus_to_relieve_repression_on_cellulase_production_and_to_adjust_the_ratio_of_cellulolytic_enzymes_for_more_efficient_hydrolysis_of_lignocellulosic_biomass_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1656(18)30628-X DB - PRIME DP - Unbound Medicine ER -