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Synergistic effects of dietary Bacillus sp. SJ-10 plus β-glucooligosaccharides as a synbiotic on growth performance, innate immunity and streptococcosis resistance in olive flounder (Paralichthys olivaceus).
Fish Shellfish Immunol. 2018 Nov; 82:544-553.FS

Abstract

Bacillus sp. SJ-10 (BSJ-10) was identified from traditional Korean fermented fish, the previously recognized prebiotic β-glucooligosaccharides (BGO), and their combination as a synbiotic were prepared to evaluate their individual and synergistic effects in olive flounder (Paralichthys olivaceus). Four diets (one control and three treatments) were formulated containing neither BSJ-10 nor BGO (control), 1 × 108 CFU g -1 BSJ-10 (BSJ-10), 0.1% BGO (BGO), and 1 × 108 CFU g-1 BSJ-10 + 0.1% BGO (BSJ-10 + BGO). Triplicates of 15 fish (weight 10 ± 0.25 g) were randomly allocated to the four diet groups and fed one of the diets for 8 weeks. At the end of the experiment, fish weight gain (WG), specific growth rate (SGR), feed conversion ratio, and protein efficiency ratio in BSJ-10, BGO and BSJ-10 + BGO diets were positively modulated (P < 0.05) compared with control. Specially, WG and SGR were significantly (P < 0.05) higher in BSJ-10 + BGO than that of BSJ-10 and BGO (individual component). The innate immune parameters such as respiratory burst, superoxide dismutase, and lysozyme activity (LSZ) of fish fed BSJ-10 and BSJ-10 + BGO (both groups) were significantly (P < 0.05) higher than the control. Moreover, myeloperoxidase activity (MPO) and LSZ of fish fed BSJ-10 + BGO were significantly higher compared with individual component. Compared with control, intestinal BSJ-10 content, expression of interleukin (IL)-1β in liver and kidney, and tumor necrosis factor (TNF)-α in liver were higher in both groups, but microvillus length was increased (P < 0.05) only in BSJ-10 + BGO. During in vivo challenge experiment with Streptococcus iniae (1 × 108 CFU ml-1), survival rate of fish was significantly higher in all treatment groups versus control. Moreover, in BSJ-10 + BGO, protection against S. iniae infection and transcription of TNF-α and IL-6 in gill were significantly (P < 0.05) higher than the individual component. Collectively, an improved WG, SGR, MPO, LSZ, transcription of IL-6 and TNF-α, and cumulative survival rate against streptococcosis clearly demonstrates a synergistic outcome of diet BSJ-10 + BGO as synbiotic in olive flounder.

Authors+Show Affiliations

Department of Biotechnology, Pukyong National University, Busan, 608-737, Republic of Korea.Department of Biotechnology, Pukyong National University, Busan, 608-737, Republic of Korea.Department of Marine Bio-Materials and Aquaculture, Pukyong National University, Busan, 608-737, Republic of Korea.Aquafeed Research Center, NIFS, Pohang, 791-923, Republic of Korea.Aquafeed Research Center, NIFS, Pohang, 791-923, Republic of Korea.Aquafeed Research Center, NIFS, Pohang, 791-923, Republic of Korea.Aquafeed Research Center, NIFS, Pohang, 791-923, Republic of Korea.Department of Marine Bio-Materials and Aquaculture, Pukyong National University, Busan, 608-737, Republic of Korea.Department of Biotechnology, Pukyong National University, Busan, 608-737, Republic of Korea. Electronic address: iskong@pknu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30194054

Citation

Hasan, Md Tawheed, et al. "Synergistic Effects of Dietary Bacillus Sp. SJ-10 Plus Β-glucooligosaccharides as a Synbiotic On Growth Performance, Innate Immunity and Streptococcosis Resistance in Olive Flounder (Paralichthys Olivaceus)." Fish & Shellfish Immunology, vol. 82, 2018, pp. 544-553.
Hasan MT, Jang WJ, Kim H, et al. Synergistic effects of dietary Bacillus sp. SJ-10 plus β-glucooligosaccharides as a synbiotic on growth performance, innate immunity and streptococcosis resistance in olive flounder (Paralichthys olivaceus). Fish Shellfish Immunol. 2018;82:544-553.
Hasan, M. T., Jang, W. J., Kim, H., Lee, B. J., Kim, K. W., Hur, S. W., Lim, S. G., Bai, S. C., & Kong, I. S. (2018). Synergistic effects of dietary Bacillus sp. SJ-10 plus β-glucooligosaccharides as a synbiotic on growth performance, innate immunity and streptococcosis resistance in olive flounder (Paralichthys olivaceus). Fish & Shellfish Immunology, 82, 544-553. https://doi.org/10.1016/j.fsi.2018.09.002
Hasan MT, et al. Synergistic Effects of Dietary Bacillus Sp. SJ-10 Plus Β-glucooligosaccharides as a Synbiotic On Growth Performance, Innate Immunity and Streptococcosis Resistance in Olive Flounder (Paralichthys Olivaceus). Fish Shellfish Immunol. 2018;82:544-553. PubMed PMID: 30194054.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synergistic effects of dietary Bacillus sp. SJ-10 plus β-glucooligosaccharides as a synbiotic on growth performance, innate immunity and streptococcosis resistance in olive flounder (Paralichthys olivaceus). AU - Hasan,Md Tawheed, AU - Jang,Won Je, AU - Kim,Haham, AU - Lee,Bong-Joo, AU - Kim,Kang Woong, AU - Hur,Sang Woo, AU - Lim,Sang Gu, AU - Bai,Sungchul C, AU - Kong,In-Soo, Y1 - 2018/09/04/ PY - 2018/05/01/received PY - 2018/07/23/revised PY - 2018/09/03/accepted PY - 2018/9/9/pubmed PY - 2019/1/4/medline PY - 2018/9/9/entrez KW - Gene expression KW - Growth performance KW - Innate immunity KW - Olive flounder KW - Synbiotic SP - 544 EP - 553 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 82 N2 - Bacillus sp. SJ-10 (BSJ-10) was identified from traditional Korean fermented fish, the previously recognized prebiotic β-glucooligosaccharides (BGO), and their combination as a synbiotic were prepared to evaluate their individual and synergistic effects in olive flounder (Paralichthys olivaceus). Four diets (one control and three treatments) were formulated containing neither BSJ-10 nor BGO (control), 1 × 108 CFU g -1 BSJ-10 (BSJ-10), 0.1% BGO (BGO), and 1 × 108 CFU g-1 BSJ-10 + 0.1% BGO (BSJ-10 + BGO). Triplicates of 15 fish (weight 10 ± 0.25 g) were randomly allocated to the four diet groups and fed one of the diets for 8 weeks. At the end of the experiment, fish weight gain (WG), specific growth rate (SGR), feed conversion ratio, and protein efficiency ratio in BSJ-10, BGO and BSJ-10 + BGO diets were positively modulated (P < 0.05) compared with control. Specially, WG and SGR were significantly (P < 0.05) higher in BSJ-10 + BGO than that of BSJ-10 and BGO (individual component). The innate immune parameters such as respiratory burst, superoxide dismutase, and lysozyme activity (LSZ) of fish fed BSJ-10 and BSJ-10 + BGO (both groups) were significantly (P < 0.05) higher than the control. Moreover, myeloperoxidase activity (MPO) and LSZ of fish fed BSJ-10 + BGO were significantly higher compared with individual component. Compared with control, intestinal BSJ-10 content, expression of interleukin (IL)-1β in liver and kidney, and tumor necrosis factor (TNF)-α in liver were higher in both groups, but microvillus length was increased (P < 0.05) only in BSJ-10 + BGO. During in vivo challenge experiment with Streptococcus iniae (1 × 108 CFU ml-1), survival rate of fish was significantly higher in all treatment groups versus control. Moreover, in BSJ-10 + BGO, protection against S. iniae infection and transcription of TNF-α and IL-6 in gill were significantly (P < 0.05) higher than the individual component. Collectively, an improved WG, SGR, MPO, LSZ, transcription of IL-6 and TNF-α, and cumulative survival rate against streptococcosis clearly demonstrates a synergistic outcome of diet BSJ-10 + BGO as synbiotic in olive flounder. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/30194054/Synergistic_effects_of_dietary_Bacillus_sp__SJ_10_plus_β_glucooligosaccharides_as_a_synbiotic_on_growth_performance_innate_immunity_and_streptococcosis_resistance_in_olive_flounder__Paralichthys_olivaceus__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(18)30538-2 DB - PRIME DP - Unbound Medicine ER -