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The transient receptor potential cation channel subfamily V members 1 and 2, P2X purinoceptor 3 and calcitonin gene-related peptide in sensory neurons of the rat trigeminal ganglion, innervating the periosteum, masseter muscle and facial skin.
Arch Oral Biol. 2018 Dec; 96:66-73.AO

Abstract

OBJECTIVE

Distribution of the transient receptor potential cation channel subfamily V members 1 (TRPV1) and 2 (TRPV2), and P2X purinoceptor 3 (P2 × 3) was investigated in rat trigeminal ganglion neurons innervating the periosteum, masseter muscle and facial skin.

DESIGN

Double immunofluorescence method for TRPV1 and TRPV2 ion channels or ATP receptor P2 × 3 with calcitonin gene-related peptide (CGRP) was performed on trigeminal ganglion neurons retrogradely labeled from the mandibular periosteum, masseter muscle, or facial skin in 15 male Wistar rats.

RESULTS

The cell size of periosteum neurons (mean ± S.D. = 810.7 ± 36.1 μ m2) was smaller than that of masseter muscle neurons (927.0 ± 75.6 μ m2), and larger than that of facial skin neurons (661.3 ± 82.2 μ m2). Periosteum neurons contained TRPV1- (26.7%), TRPV2- (47.1%) and P2 × 3-immunoreactivity (50.1%). Expression of TRPV2-immunoreactivity was more abundant among periosteum neurons than among facial skin neurons (16.1%). Regarding to TRPV1 and P2 × 3 expression, however, there was no significant difference between periosteum neurons and, masseter muscle and facial skin neurons. TRPV1- immunoreactive trigeminal ganglion neurons which innervated the periosteum, masseter muscle and facial skin mostly had small and medium-sized cell bodies, whereas TRPV2- and P2 × 3-immunoreactive trigeminal ganglion neurons innervating those tissues were of various cell body sizes. Approximately 20% of periosteum (19.2%), masseter muscle (19.2%) and facial skin (21.5%) neurons contained both TRPV1- and CGRP-immunoreactivity. Some periosteum neurons also co-expressed CGRP-immunoreactivity with TRPV2- (10.9%) or P2 × 3- immunoreactivity (11.1%). Distributions of perivascular and free nerve fibers containing CGRP and either TRPV1, TRPV2, or P2 × 3 were occasionally very similar in the mandibular periosteum.

CONCLUSIONS

The present study indicated that trigeminal ganglion nociceptors innervating the periosteum as well as those innervating the masseter muscle and facial skin have vanilloid, acidic, thermal, mechanical and ATP sensors. In some periosteum neurons, CGRP may act as inflammatory mediator through activation of TRPV1, TRPV2 and P2 × 3.

Authors+Show Affiliations

Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku University, Sendai, 980-8575, Japan.Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku University, Sendai, 980-8575, Japan.Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku University, Sendai, 980-8575, Japan.Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku University, Sendai, 980-8575, Japan.Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku University, Sendai, 980-8575, Japan. Electronic address: hiroichi@anat.dent.tohoku.ac.jp.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30195141

Citation

Sato, Maki, et al. "The Transient Receptor Potential Cation Channel Subfamily V Members 1 and 2, P2X Purinoceptor 3 and Calcitonin Gene-related Peptide in Sensory Neurons of the Rat Trigeminal Ganglion, Innervating the Periosteum, Masseter Muscle and Facial Skin." Archives of Oral Biology, vol. 96, 2018, pp. 66-73.
Sato M, Sato T, Yajima T, et al. The transient receptor potential cation channel subfamily V members 1 and 2, P2X purinoceptor 3 and calcitonin gene-related peptide in sensory neurons of the rat trigeminal ganglion, innervating the periosteum, masseter muscle and facial skin. Arch Oral Biol. 2018;96:66-73.
Sato, M., Sato, T., Yajima, T., Shimazaki, K., & Ichikawa, H. (2018). The transient receptor potential cation channel subfamily V members 1 and 2, P2X purinoceptor 3 and calcitonin gene-related peptide in sensory neurons of the rat trigeminal ganglion, innervating the periosteum, masseter muscle and facial skin. Archives of Oral Biology, 96, 66-73. https://doi.org/10.1016/j.archoralbio.2018.08.012
Sato M, et al. The Transient Receptor Potential Cation Channel Subfamily V Members 1 and 2, P2X Purinoceptor 3 and Calcitonin Gene-related Peptide in Sensory Neurons of the Rat Trigeminal Ganglion, Innervating the Periosteum, Masseter Muscle and Facial Skin. Arch Oral Biol. 2018;96:66-73. PubMed PMID: 30195141.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The transient receptor potential cation channel subfamily V members 1 and 2, P2X purinoceptor 3 and calcitonin gene-related peptide in sensory neurons of the rat trigeminal ganglion, innervating the periosteum, masseter muscle and facial skin. AU - Sato,Maki, AU - Sato,Tadasu, AU - Yajima,Takehiro, AU - Shimazaki,Kenichiro, AU - Ichikawa,Hiroyuki, Y1 - 2018/08/30/ PY - 2018/06/26/received PY - 2018/08/17/revised PY - 2018/08/22/accepted PY - 2018/9/9/pubmed PY - 2019/3/28/medline PY - 2018/9/9/entrez KW - Immunohistochemistry KW - P2X3 KW - Periosteum KW - TRPV1 KW - TRPV2 KW - Trigeminal ganglion SP - 66 EP - 73 JF - Archives of oral biology JO - Arch. Oral Biol. VL - 96 N2 - OBJECTIVE: Distribution of the transient receptor potential cation channel subfamily V members 1 (TRPV1) and 2 (TRPV2), and P2X purinoceptor 3 (P2 × 3) was investigated in rat trigeminal ganglion neurons innervating the periosteum, masseter muscle and facial skin. DESIGN: Double immunofluorescence method for TRPV1 and TRPV2 ion channels or ATP receptor P2 × 3 with calcitonin gene-related peptide (CGRP) was performed on trigeminal ganglion neurons retrogradely labeled from the mandibular periosteum, masseter muscle, or facial skin in 15 male Wistar rats. RESULTS: The cell size of periosteum neurons (mean ± S.D. = 810.7 ± 36.1 μ m2) was smaller than that of masseter muscle neurons (927.0 ± 75.6 μ m2), and larger than that of facial skin neurons (661.3 ± 82.2 μ m2). Periosteum neurons contained TRPV1- (26.7%), TRPV2- (47.1%) and P2 × 3-immunoreactivity (50.1%). Expression of TRPV2-immunoreactivity was more abundant among periosteum neurons than among facial skin neurons (16.1%). Regarding to TRPV1 and P2 × 3 expression, however, there was no significant difference between periosteum neurons and, masseter muscle and facial skin neurons. TRPV1- immunoreactive trigeminal ganglion neurons which innervated the periosteum, masseter muscle and facial skin mostly had small and medium-sized cell bodies, whereas TRPV2- and P2 × 3-immunoreactive trigeminal ganglion neurons innervating those tissues were of various cell body sizes. Approximately 20% of periosteum (19.2%), masseter muscle (19.2%) and facial skin (21.5%) neurons contained both TRPV1- and CGRP-immunoreactivity. Some periosteum neurons also co-expressed CGRP-immunoreactivity with TRPV2- (10.9%) or P2 × 3- immunoreactivity (11.1%). Distributions of perivascular and free nerve fibers containing CGRP and either TRPV1, TRPV2, or P2 × 3 were occasionally very similar in the mandibular periosteum. CONCLUSIONS: The present study indicated that trigeminal ganglion nociceptors innervating the periosteum as well as those innervating the masseter muscle and facial skin have vanilloid, acidic, thermal, mechanical and ATP sensors. In some periosteum neurons, CGRP may act as inflammatory mediator through activation of TRPV1, TRPV2 and P2 × 3. SN - 1879-1506 UR - https://www.unboundmedicine.com/medline/citation/30195141/The_transient_receptor_potential_cation_channel_subfamily_V_members_1_and_2_P2X_purinoceptor_3_and_calcitonin_gene_related_peptide_in_sensory_neurons_of_the_rat_trigeminal_ganglion_innervating_the_periosteum_masseter_muscle_and_facial_skin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9969(18)30308-X DB - PRIME DP - Unbound Medicine ER -