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Rapid and persistent RNAi response in western corn rootworm adults.
Pestic Biochem Physiol. 2018 Sep; 150:66-70.PB

Abstract

RNA interference (RNAi) has proven effective for controlling pest insects such as western corn rootworm (WCR), Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae). Previous studies have shown that WCR adults display a robust RNAi response to orally-administered double-stranded RNA (dsRNA). However, it is unclear how quickly the response occurs after ingestion or how long RNAi effect lasts after WCR stop ingesting diet containing dsRNA. In the current study, WCR adult females were provided with diet treated with dsRNAs of Laccase 2 and Argonaute 2, two nonessential genes, for 8 days. RNAi response in WCR females commenced as early as 10 h after exposure to dsRNA and lasted up to 40 days after exposure to dsRNA ended. Our results show that dsRNA-mediated RNAi response in WCR females is rapid and long-lasting. These findings suggest that even a short-term ingestion of transgenic plants expressing dsRNA by WCR may have a sustained impact on this insect.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wu K
Department of Entomology and Nematology, PO Box 11620, University of Florida, Gainesville, FL 32611, USA. Electronic address: kewu@ufl.edu.
Taylor CE
Department of Entomology and Nematology, PO Box 11620, University of Florida, Gainesville, FL 32611, USA.
Fishilevich E
Dow AgroSciences LLC, 9330 Zionsville Rd, Indianapolis, IN 46268, USA.
Narva KE
Dow AgroSciences LLC, 9330 Zionsville Rd, Indianapolis, IN 46268, USA.
Siegfried BD
Department of Entomology and Nematology, PO Box 11620, University of Florida, Gainesville, FL 32611, USA.

MeSH

AnimalsArgonaute ProteinsColeopteraFemaleGene Knockdown TechniquesGene SilencingInsect ProteinsLaccasePest Control, BiologicalRNA InterferenceRNA, Double-StrandedReverse Transcriptase Polymerase Chain Reaction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30195389

Citation

Wu, Ke, et al. "Rapid and Persistent RNAi Response in Western Corn Rootworm Adults." Pesticide Biochemistry and Physiology, vol. 150, 2018, pp. 66-70.
Wu K, Taylor CE, Fishilevich E, et al. Rapid and persistent RNAi response in western corn rootworm adults. Pestic Biochem Physiol. 2018;150:66-70.
Wu, K., Taylor, C. E., Fishilevich, E., Narva, K. E., & Siegfried, B. D. (2018). Rapid and persistent RNAi response in western corn rootworm adults. Pesticide Biochemistry and Physiology, 150, 66-70. https://doi.org/10.1016/j.pestbp.2018.07.002
Wu K, et al. Rapid and Persistent RNAi Response in Western Corn Rootworm Adults. Pestic Biochem Physiol. 2018;150:66-70. PubMed PMID: 30195389.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and persistent RNAi response in western corn rootworm adults. AU - Wu,Ke, AU - Taylor,Caitlin E, AU - Fishilevich,Elane, AU - Narva,Kenneth E, AU - Siegfried,Blair D, Y1 - 2018/07/10/ PY - 2018/03/28/received PY - 2018/06/28/revised PY - 2018/07/08/accepted PY - 2018/9/10/entrez PY - 2018/9/10/pubmed PY - 2018/11/2/medline SP - 66 EP - 70 JF - Pesticide biochemistry and physiology JO - Pestic Biochem Physiol VL - 150 N2 - RNA interference (RNAi) has proven effective for controlling pest insects such as western corn rootworm (WCR), Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae). Previous studies have shown that WCR adults display a robust RNAi response to orally-administered double-stranded RNA (dsRNA). However, it is unclear how quickly the response occurs after ingestion or how long RNAi effect lasts after WCR stop ingesting diet containing dsRNA. In the current study, WCR adult females were provided with diet treated with dsRNAs of Laccase 2 and Argonaute 2, two nonessential genes, for 8 days. RNAi response in WCR females commenced as early as 10 h after exposure to dsRNA and lasted up to 40 days after exposure to dsRNA ended. Our results show that dsRNA-mediated RNAi response in WCR females is rapid and long-lasting. These findings suggest that even a short-term ingestion of transgenic plants expressing dsRNA by WCR may have a sustained impact on this insect. SN - 1095-9939 UR - https://www.unboundmedicine.com/medline/citation/30195389/Rapid_and_persistent_RNAi_response_in_western_corn_rootworm_adults_ DB - PRIME DP - Unbound Medicine ER -