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In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum.
J Integr Med. 2018 Nov; 16(6):404-410.JI

Abstract

OBJECTIVE

In the present study, we evaluated the effects of the aqueous extract of Physalis angulata root (AEPa) on Leishmania infantum proliferation, morphology, and the driving mechanism in leishmanicidal activity and modulatory action on macrophages.

METHODS

L. infantum promastigotes were treated with 50 and 100 µg/mL AEPa for 72 h and then antipromastigote assay was performed by counts in a Newbauer chamber, morphological changes were analyzed by transmission electron microscopy and the mechanism of the leishmanicidal activity was detected. In addition, macrophages were infected with L. infantum and were used to evaluate anti-amastigote activity of AEPa and effects of AEPa on cytokine secretion after 72-hour treatment.

RESULTS

Treatment with AEPa reduced the numbers of L. infantum promastigotes (50% inhibitory concentration (IC50) = 65.9 μg/mL; selectivity index (SI) = 22.1) and amastigotes (IC50 = 37.9 μg/mL; SI = 38.5) compared with the untreated control. Amphotericin B reduced 100% of the promastigote numbers after 72 h of treatment (IC50 = 0.2 μg/mL). AEPa induced several morphological changes and increased the production of reactive oxygen species and apoptotic death in promastigotes after treating for 72 h. AEPa (100 μg/mL) promoted tumor necrosis factor-α secretion in macrophages infected with L. infantum after 72 h of treatment, but did not induce an increase in this cytokine in noninfected macrophages. In addition, AEPa showed no cytotoxic effect on J774-A1 cells (50% cytotoxic concentration >1000 μg/mL).

CONCLUSION

AEPa presented antileishmanial activity against the promastigotes and amastigotes of L. infantum without macrophage cytotoxicity; these results show that natural products such as P. angulata have leishmanicidal potential and in the future may be an alternative treatment for leishmaniasis.

Authors+Show Affiliations

Laboratory of Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará 66075-110, Brazil; National Institute of Science and Technology in Structural Biology and Bioimaging (INCT-INBEB), Rio de Janeiro 21941-901, Brazil.Laboratory of Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará 66075-110, Brazil.Laboratory of Electron Microscopy, Department of Health Surveillance, Ministry of Health, Evandro Chagas Institute, Belém, Pará 66087-082, Brazil.Laboratory of Molecular and Cellular Neurochemistry, Institute of Biological Sciences, Federal University of Pará, Belém, Pará 66075-110, Brazil; National Institute of Science and Technology in Neuroimmunomodulation (INCT-NIM), Rio de Janeiro 21040-360, Brazil.Laboratory of Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Pará 66075-110, Brazil; National Institute of Science and Technology in Structural Biology and Bioimaging (INCT-INBEB), Rio de Janeiro 21941-901, Brazil. Electronic address: edilene@ufpa.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30195443

Citation

Da Silva, Bruno José Martins, et al. "In Vitro Antileishmanial Effects of Physalis Angulata Root Extract On Leishmania Infantum." Journal of Integrative Medicine, vol. 16, no. 6, 2018, pp. 404-410.
Da Silva BJM, Pereira SWG, Rodrigues APD, et al. In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum. J Integr Med. 2018;16(6):404-410.
Da Silva, B. J. M., Pereira, S. W. G., Rodrigues, A. P. D., Do Nascimento, J. L. M., & Silva, E. O. (2018). In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum. Journal of Integrative Medicine, 16(6), 404-410. https://doi.org/10.1016/j.joim.2018.08.004
Da Silva BJM, et al. In Vitro Antileishmanial Effects of Physalis Angulata Root Extract On Leishmania Infantum. J Integr Med. 2018;16(6):404-410. PubMed PMID: 30195443.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum. AU - Da Silva,Bruno José Martins, AU - Pereira,Sandro Wilson Gomes, AU - Rodrigues,Ana Paula Drummond, AU - Do Nascimento,José Luiz Martins, AU - Silva,Edilene Oliveira, Y1 - 2018/08/25/ PY - 2018/02/27/received PY - 2018/05/14/accepted PY - 2018/9/10/pubmed PY - 2019/8/29/medline PY - 2018/9/10/entrez KW - Apoptosis KW - Leishmania infantum KW - Leishmanicidal activity KW - Physalis angulata KW - Reactive oxygen species SP - 404 EP - 410 JF - Journal of integrative medicine JO - J Integr Med VL - 16 IS - 6 N2 - OBJECTIVE: In the present study, we evaluated the effects of the aqueous extract of Physalis angulata root (AEPa) on Leishmania infantum proliferation, morphology, and the driving mechanism in leishmanicidal activity and modulatory action on macrophages. METHODS: L. infantum promastigotes were treated with 50 and 100 µg/mL AEPa for 72 h and then antipromastigote assay was performed by counts in a Newbauer chamber, morphological changes were analyzed by transmission electron microscopy and the mechanism of the leishmanicidal activity was detected. In addition, macrophages were infected with L. infantum and were used to evaluate anti-amastigote activity of AEPa and effects of AEPa on cytokine secretion after 72-hour treatment. RESULTS: Treatment with AEPa reduced the numbers of L. infantum promastigotes (50% inhibitory concentration (IC50) = 65.9 μg/mL; selectivity index (SI) = 22.1) and amastigotes (IC50 = 37.9 μg/mL; SI = 38.5) compared with the untreated control. Amphotericin B reduced 100% of the promastigote numbers after 72 h of treatment (IC50 = 0.2 μg/mL). AEPa induced several morphological changes and increased the production of reactive oxygen species and apoptotic death in promastigotes after treating for 72 h. AEPa (100 μg/mL) promoted tumor necrosis factor-α secretion in macrophages infected with L. infantum after 72 h of treatment, but did not induce an increase in this cytokine in noninfected macrophages. In addition, AEPa showed no cytotoxic effect on J774-A1 cells (50% cytotoxic concentration >1000 μg/mL). CONCLUSION: AEPa presented antileishmanial activity against the promastigotes and amastigotes of L. infantum without macrophage cytotoxicity; these results show that natural products such as P. angulata have leishmanicidal potential and in the future may be an alternative treatment for leishmaniasis. SN - 2095-4964 UR - https://www.unboundmedicine.com/medline/citation/30195443/In_vitro_antileishmanial_effects_of_Physalis_angulata_root_extract_on_Leishmania_infantum_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2095-4964(18)30097-9 DB - PRIME DP - Unbound Medicine ER -