Tags

Type your tag names separated by a space and hit enter

In vitro assessment of the acaricidal activity of carvacrol, thymol, eugenol and their acetylated derivatives on Rhipicephalus microplus (Acari: Ixodidae).
Vet Parasitol. 2018 Aug 30; 260:1-4.VP

Abstract

This study reports the comparative evaluation of the activity of carvacrol, thymol, eugenol and their respective acetylated derivatives (carvacrol acetate, thymol acetate and eugenol acetate) on Rhipicephalus microplus, to verify the possible influence of the acetate group. The acetylated derivatives were prepared from reactions of the phenolic compounds with acetic anhydride/pyridine. The formation of the products was confirmed by analysis of hydrogen and carbon-13 nuclear magnetic resonance (1H and 13C NMR) spectra. The larval packet test was used to evaluate the acaricidal activity, with concentrations of 0.312, 0.625, 1.25, 2.5, 5.0, 7.5, 10.0 and 15.0 mg/mL. Thymol and carvacrol resulted in 100% mortality starting at the concentration of 2.5 mg/mL, while the same was observed for carvacrol acetate starting at the concentration of 5.0 mg/mL. For the other treatments, 100% mortality was only achieved in the groups treated with the highest concentration (15.0 mg/mL). The LC50 and LC90 values (mg/mL) of carvacrol acetate (2.49, 4.21), thymol acetate (2.97, 8.52) and eugenol acetate (4.25, 13.10) were higher than those for the corresponding precursor molecules carvacrol (0.83, 2.02), thymol (1.26, 2.21) and eugenol (2.77, 5.35). The acetylation process did not enhance the activity of these substances on unengorged larvae of R. microplus, since the precursor substances (carvacrol, thymol and eugenol) had greater efficacy.

Authors+Show Affiliations

Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro (UFRRJ), 23.890-000, Seropédica, RJ, Brazil. Electronic address: tatinovatojf@gmail.com.Centro de Ciências Exatas e Tecnologia, Universidade Estadual Vale do Acaraú, 62040-730, Sobral, Ceará, Brazil.Programa de Pós-graduação em Ciência Animal, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Av. Esperança, s/n, Campus Samambaia, 74690-900, Goiânia, Goiás, Brazil.Programa de Pós-graduação em Ciências Biológicas, Comportamento e Biologia Animal, Universidade Federal de Juiz de Fora, 36.036-900, Juiz de Fora, MG, Brazil.Departamento de Química, Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, 23.890-000, BR-465, Km 7 - Seropédica, Rio de Janeiro, Brazil.Programa de Pós-graduação em Biologia Animal da Universidade Federal Rural do Rio de Janeiro, 23890-000, BR-465, Km 7 - Seropédica, Rio de Janeiro, Brazil.Departamento de Química, Instituto de Ciências Exatas, Universidade Federal Rural do Rio de Janeiro, 23.890-000, BR-465, Km 7 - Seropédica, Rio de Janeiro, Brazil.Programa de Pós-graduação em Ciências Biológicas, Comportamento e Biologia Animal, Universidade Federal de Juiz de Fora, 36.036-900, Juiz de Fora, MG, Brazil.Programa de Pós-graduação em Ciência Animal, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Av. Esperança, s/n, Campus Samambaia, 74690-900, Goiânia, Goiás, Brazil; Instituto de Patologia Tropical e Saúde Pública da Universidade Federal de Goiás, 74605-050, rua 35, s/n, Goiânia, Goiás, Brazil.

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

30197007

Citation

Novato, Tatiane, et al. "In Vitro Assessment of the Acaricidal Activity of Carvacrol, Thymol, Eugenol and Their Acetylated Derivatives On Rhipicephalus Microplus (Acari: Ixodidae)." Veterinary Parasitology, vol. 260, 2018, pp. 1-4.
Novato T, Gomes GA, Zeringóta V, et al. In vitro assessment of the acaricidal activity of carvacrol, thymol, eugenol and their acetylated derivatives on Rhipicephalus microplus (Acari: Ixodidae). Vet Parasitol. 2018;260:1-4.
Novato, T., Gomes, G. A., Zeringóta, V., Franco, C. T., de Oliveira, D. R., Melo, D., de Carvalho, M. G., Daemon, E., & de Oliveira Monteiro, C. M. (2018). In vitro assessment of the acaricidal activity of carvacrol, thymol, eugenol and their acetylated derivatives on Rhipicephalus microplus (Acari: Ixodidae). Veterinary Parasitology, 260, 1-4. https://doi.org/10.1016/j.vetpar.2018.07.009
Novato T, et al. In Vitro Assessment of the Acaricidal Activity of Carvacrol, Thymol, Eugenol and Their Acetylated Derivatives On Rhipicephalus Microplus (Acari: Ixodidae). Vet Parasitol. 2018 Aug 30;260:1-4. PubMed PMID: 30197007.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro assessment of the acaricidal activity of carvacrol, thymol, eugenol and their acetylated derivatives on Rhipicephalus microplus (Acari: Ixodidae). AU - Novato,Tatiane, AU - Gomes,Geovany Amorim, AU - Zeringóta,Viviane, AU - Franco,Cristiane Teixeira, AU - de Oliveira,Débora Ramos, AU - Melo,Diego, AU - de Carvalho,Mário Geraldo, AU - Daemon,Erik, AU - de Oliveira Monteiro,Caio Márcio, Y1 - 2018/07/18/ PY - 2018/04/10/received PY - 2018/07/17/revised PY - 2018/07/18/accepted PY - 2018/9/11/entrez PY - 2018/9/11/pubmed PY - 2019/1/31/medline KW - Carvacrol acetate KW - Cattle tick KW - Eugenol acetate KW - Thymol acetate SP - 1 EP - 4 JF - Veterinary parasitology JO - Vet. Parasitol. VL - 260 N2 - This study reports the comparative evaluation of the activity of carvacrol, thymol, eugenol and their respective acetylated derivatives (carvacrol acetate, thymol acetate and eugenol acetate) on Rhipicephalus microplus, to verify the possible influence of the acetate group. The acetylated derivatives were prepared from reactions of the phenolic compounds with acetic anhydride/pyridine. The formation of the products was confirmed by analysis of hydrogen and carbon-13 nuclear magnetic resonance (1H and 13C NMR) spectra. The larval packet test was used to evaluate the acaricidal activity, with concentrations of 0.312, 0.625, 1.25, 2.5, 5.0, 7.5, 10.0 and 15.0 mg/mL. Thymol and carvacrol resulted in 100% mortality starting at the concentration of 2.5 mg/mL, while the same was observed for carvacrol acetate starting at the concentration of 5.0 mg/mL. For the other treatments, 100% mortality was only achieved in the groups treated with the highest concentration (15.0 mg/mL). The LC50 and LC90 values (mg/mL) of carvacrol acetate (2.49, 4.21), thymol acetate (2.97, 8.52) and eugenol acetate (4.25, 13.10) were higher than those for the corresponding precursor molecules carvacrol (0.83, 2.02), thymol (1.26, 2.21) and eugenol (2.77, 5.35). The acetylation process did not enhance the activity of these substances on unengorged larvae of R. microplus, since the precursor substances (carvacrol, thymol and eugenol) had greater efficacy. SN - 1873-2550 UR - https://www.unboundmedicine.com/medline/citation/30197007/In_vitro_assessment_of_the_acaricidal_activity_of_carvacrol_thymol_eugenol_and_their_acetylated_derivatives_on_Rhipicephalus_microplus__Acari:_Ixodidae__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-4017(18)30277-2 DB - PRIME DP - Unbound Medicine ER -