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Physiochemical properties of Trichoderma longibrachiatum DSMZ 16517-synthesized silver nanoparticles for the mitigation of halotolerant sulphate-reducing bacteria.
J Appl Microbiol. 2019 Jan; 126(1):138-154.JA

Abstract

AIMS

In order to efficiently control the corrosive sulphate-reducing bacteria (SRB), the main precursor of the microbial influenced corrosion (MIC) in oil industry, the ability of Trichoderma longibrachiatumDSMZ 16517 to synthesize silver nanoparticles (AgNPs) was investigated and their biocidal activity against halotolerant SRB was tested.

METHODS AND RESULTS

The mycelial cell-free filtrate (MCFF) bioreduced the silver ions (Ag+) to their metallic nanoparticle state (Ag0), which was presumptively indicated by the appearance of a dark brown suspension and confirmed by the characteristic absorbance of AgNPs at ʎ422nm . One-factor-at-a-time technique was used to optimize the effect of temperature, time, pH, fungal biomass and silver nitrate concentrations, stirring rates and dark effect. The dynamic light scattering (DLS) analysis revealed average AgNPs size and zeta potential values of 17·75 nm and -26·8 mV, respectively, indicating the stability of the prepared AgNPs. The X-ray diffraction (XRD) pattern assured the crystallinity of the mycosynthesized AgNPs, with an average size of 61 nm. The field emission scanning electron microscope (FESEM) and high-resolution transmission electron microscope (HRTEM) showed nonagglomerated spherical, triangular and cuboid AgNPs ranging from 5 to 11 ± 0·5 nm. The Fourier transform infrared spectroscopy (FT-IR) analysis of the mycosynthesized AgNPs affirmed the role of MCFF as a reducing and capping agent. A preliminary suggested mechanism for mycosynthesis of AgNPs was elucidated. The mycosynthesized AgNPs expressed high biocidal activity against a halotolerant planktonic mixed culture of SRB. The HRTEM analysis showed a clear evidence of an alteration in cell morphology, a disruption of SRB cell membranes, a lysis in cell wall and a cytoplasmic extraction after treatment with AgNPs. This confirmed the bactericidal effect of the mycosynthesized AgNPs.

CONCLUSION

The biocidal activity of the mycosynthesized AgNPs against halotolerant planktonic SRB makes it an attractive option to control MIC in the petroleum industry.

SIGNIFICANCE AND IMPACT OF THE STUDY

This research provides a helpful insight into the development of a new mycosynthesized biocidal agent against the corrosive sulphate-reducing bacteria.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Egyptian Petroleum Research Institute, Cairo, Egypt.

Egyptian Petroleum Research Institute, Cairo, Egypt.

Egyptian Petroleum Research Institute, Cairo, Egypt.

Egyptian Petroleum Research Institute, Cairo, Egypt.

Egyptian Petroleum Research Institute, Cairo, Egypt.

Faculty of Science, Ain Shams University, Cairo, Egypt.

Egyptian Petroleum Research Institute, Cairo, Egypt.

MeSH

Anti-Bacterial AgentsBacteriaMetal NanoparticlesMicroscopy, Electron, TransmissionOxidation-ReductionSilverSodium ChlorideSpectroscopy, Fourier Transform InfraredSulfatesTrichodermaX-Ray Diffraction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30199141

Citation

Omran, B A., et al. "Physiochemical Properties of Trichoderma Longibrachiatum DSMZ 16517-synthesized Silver Nanoparticles for the Mitigation of Halotolerant Sulphate-reducing Bacteria." Journal of Applied Microbiology, vol. 126, no. 1, 2019, pp. 138-154.

Omran BA, Nassar HN, Younis SA, et al. Physiochemical properties of Trichoderma longibrachiatum DSMZ 16517-synthesized silver nanoparticles for the mitigation of halotolerant sulphate-reducing bacteria. J Appl Microbiol. 2019;126(1):138-154.

Omran, B. A., Nassar, H. N., Younis, S. A., Fatthallah, N. A., Hamdy, A., El-Shatoury, E. H., & El-Gendy, N. S. (2019). Physiochemical properties of Trichoderma longibrachiatum DSMZ 16517-synthesized silver nanoparticles for the mitigation of halotolerant sulphate-reducing bacteria. Journal of Applied Microbiology, 126(1), 138-154. https://doi.org/10.1111/jam.14102

Omran BA, et al. Physiochemical Properties of Trichoderma Longibrachiatum DSMZ 16517-synthesized Silver Nanoparticles for the Mitigation of Halotolerant Sulphate-reducing Bacteria. J Appl Microbiol. 2019;126(1):138-154. PubMed PMID: 30199141.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Physiochemical properties of Trichoderma longibrachiatum DSMZ 16517-synthesized silver nanoparticles for the mitigation of halotolerant sulphate-reducing bacteria. AU - Omran,B A, AU - Nassar,H N, AU - Younis,S A, AU - Fatthallah,N A, AU - Hamdy,A, AU - El-Shatoury,E H, AU - El-Gendy,N Sh, Y1 - 2018/10/09/ PY - 2018/03/09/received PY - 2018/08/21/revised PY - 2018/09/04/accepted PY - 2018/9/11/pubmed PY - 2019/2/20/medline PY - 2018/9/11/entrez KW - biocide KW - halotolerant planktonic sulphate-reducing bacteria KW - mycogenic synthesis KW - silver nanoparticles KW - surface plasmon resonance SP - 138 EP - 154 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 126 IS - 1 N2 - AIMS: In order to efficiently control the corrosive sulphate-reducing bacteria (SRB), the main precursor of the microbial influenced corrosion (MIC) in oil industry, the ability of Trichoderma longibrachiatumDSMZ 16517 to synthesize silver nanoparticles (AgNPs) was investigated and their biocidal activity against halotolerant SRB was tested. METHODS AND RESULTS: The mycelial cell-free filtrate (MCFF) bioreduced the silver ions (Ag+) to their metallic nanoparticle state (Ag0), which was presumptively indicated by the appearance of a dark brown suspension and confirmed by the characteristic absorbance of AgNPs at ʎ422nm . One-factor-at-a-time technique was used to optimize the effect of temperature, time, pH, fungal biomass and silver nitrate concentrations, stirring rates and dark effect. The dynamic light scattering (DLS) analysis revealed average AgNPs size and zeta potential values of 17·75 nm and -26·8 mV, respectively, indicating the stability of the prepared AgNPs. The X-ray diffraction (XRD) pattern assured the crystallinity of the mycosynthesized AgNPs, with an average size of 61 nm. The field emission scanning electron microscope (FESEM) and high-resolution transmission electron microscope (HRTEM) showed nonagglomerated spherical, triangular and cuboid AgNPs ranging from 5 to 11 ± 0·5 nm. The Fourier transform infrared spectroscopy (FT-IR) analysis of the mycosynthesized AgNPs affirmed the role of MCFF as a reducing and capping agent. A preliminary suggested mechanism for mycosynthesis of AgNPs was elucidated. The mycosynthesized AgNPs expressed high biocidal activity against a halotolerant planktonic mixed culture of SRB. The HRTEM analysis showed a clear evidence of an alteration in cell morphology, a disruption of SRB cell membranes, a lysis in cell wall and a cytoplasmic extraction after treatment with AgNPs. This confirmed the bactericidal effect of the mycosynthesized AgNPs. CONCLUSION: The biocidal activity of the mycosynthesized AgNPs against halotolerant planktonic SRB makes it an attractive option to control MIC in the petroleum industry. SIGNIFICANCE AND IMPACT OF THE STUDY: This research provides a helpful insight into the development of a new mycosynthesized biocidal agent against the corrosive sulphate-reducing bacteria. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/30199141/Physiochemical_properties_of_Trichoderma_longibrachiatum_DSMZ_16517_synthesized_silver_nanoparticles_for_the_mitigation_of_halotolerant_sulphate_reducing_bacteria_ DB - PRIME DP - Unbound Medicine ER -