Tags

Type your tag names separated by a space and hit enter

Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets.
Nutrients. 2018 Sep 06; 10(9)N

Abstract

Dietary fructose causes salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the filtered NaCl. Angiotensin II (Ang II), atrial natriuretic peptide (ANP) and norepinephrine (NE) regulate this process. Although Ang II signaling blockade ameliorates fructose-induced salt-sensitive hypertension, basal PT Na⁺ reabsorption and its sensitivity to the aforementioned factors have not been studied in this model. We hypothesized consuming fructose with a high-salt diet selectively enhances the sensitivity of PT transport to Ang II. We investigated the effects of Ang II, ANP and NE on PT Na reabsorption in rats fed a high-salt diet drinking tap water (HS) or 20% fructose (HS-FRU). Oxygen consumption (QO₂) was used as a measure of all ATP-dependent transport processes. Na⁺/K⁺-ATPase and Na⁺/H⁺-exchange (NHE) activities were studied because they represent primary apical and basolateral transporters in this segment. The effect of 10-12 mol/L Ang II in QO₂ by PTs from HS-FRU was larger than HS (p < 0.02; n = 7). In PTs from HS-FRU 10-12 mol/L Ang II stimulated NHE activity by 2.6 ± 0.7 arbitrary fluorescence units/s (p < 0.01; n = 5) but not in those from HS. The stimulatory effect of Ang II on PT Na⁺/K⁺-ATPase activity was not affected by HS-FRU. Responses of QO₂ and NHE activity to ANP did not differ between groups. The response of QO₂ to NE was unaltered by HS-FRU. We concluded that the sensitivity of PT Na⁺ reabsorption specifically to Ang II is enhanced by HS-FRU. This maintains high rates of transport even in the presence of low concentrations of the peptide, and likely contributes to the hypertension.

Authors+Show Affiliations

Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA. agustin.gonzalezvicente@case.edu. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires C1113AAD, Argentina. agustin.gonzalezvicente@case.edu.Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA. nxh156@case.edu.Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA. nxy66@case.edu.Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA. pdcabral@gmail.com. Facultad de Medicina, Departamento de Ciencias Fisiológicas, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires C1121ABG, Argentina. pdcabral@gmail.com. Merk Research Laboratories, 630 Gateway Blvd, South San Francisco, CA 94080, USA. pdcabral@gmail.com.Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA. jessica.berthiaume@case.edu.Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires C1113AAD, Argentina. dominici@qb.ffyb.uba.ar. Instituto de Química y Fisicoquímica Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires C1113AAD, Argentina. dominici@qb.ffyb.uba.ar.Department of Physiology and Biophysics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA. jlg5@case.edu.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

30200571

Citation

Gonzalez-Vicente, Agustin, et al. "Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets." Nutrients, vol. 10, no. 9, 2018.
Gonzalez-Vicente A, Hong NJ, Yang N, et al. Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets. Nutrients. 2018;10(9).
Gonzalez-Vicente, A., Hong, N. J., Yang, N., Cabral, P. D., Berthiaume, J. M., Dominici, F. P., & Garvin, J. L. (2018). Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets. Nutrients, 10(9). https://doi.org/10.3390/nu10091244
Gonzalez-Vicente A, et al. Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets. Nutrients. 2018 Sep 6;10(9) PubMed PMID: 30200571.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets. AU - Gonzalez-Vicente,Agustin, AU - Hong,Nancy J, AU - Yang,Nianxin, AU - Cabral,Pablo D, AU - Berthiaume,Jessica M, AU - Dominici,Fernando P, AU - Garvin,Jeffrey L, Y1 - 2018/09/06/ PY - 2018/08/08/received PY - 2018/09/04/revised PY - 2018/09/04/accepted PY - 2018/9/12/entrez PY - 2018/9/12/pubmed PY - 2018/12/18/medline KW - Na+/H+ exchange KW - Na+/K+-ATPase KW - atrial natriuretic peptide KW - blood pressure KW - kidney KW - norepinephrine KW - salt sensitivity KW - sodium excretion JF - Nutrients JO - Nutrients VL - 10 IS - 9 N2 - Dietary fructose causes salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the filtered NaCl. Angiotensin II (Ang II), atrial natriuretic peptide (ANP) and norepinephrine (NE) regulate this process. Although Ang II signaling blockade ameliorates fructose-induced salt-sensitive hypertension, basal PT Na⁺ reabsorption and its sensitivity to the aforementioned factors have not been studied in this model. We hypothesized consuming fructose with a high-salt diet selectively enhances the sensitivity of PT transport to Ang II. We investigated the effects of Ang II, ANP and NE on PT Na reabsorption in rats fed a high-salt diet drinking tap water (HS) or 20% fructose (HS-FRU). Oxygen consumption (QO₂) was used as a measure of all ATP-dependent transport processes. Na⁺/K⁺-ATPase and Na⁺/H⁺-exchange (NHE) activities were studied because they represent primary apical and basolateral transporters in this segment. The effect of 10-12 mol/L Ang II in QO₂ by PTs from HS-FRU was larger than HS (p < 0.02; n = 7). In PTs from HS-FRU 10-12 mol/L Ang II stimulated NHE activity by 2.6 ± 0.7 arbitrary fluorescence units/s (p < 0.01; n = 5) but not in those from HS. The stimulatory effect of Ang II on PT Na⁺/K⁺-ATPase activity was not affected by HS-FRU. Responses of QO₂ and NHE activity to ANP did not differ between groups. The response of QO₂ to NE was unaltered by HS-FRU. We concluded that the sensitivity of PT Na⁺ reabsorption specifically to Ang II is enhanced by HS-FRU. This maintains high rates of transport even in the presence of low concentrations of the peptide, and likely contributes to the hypertension. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/30200571/Dietary_Fructose_Increases_the_Sensitivity_of_Proximal_Tubules_to_Angiotensin_II_in_Rats_Fed_High_Salt_Diets_ L2 - http://www.mdpi.com/resolver?pii=nu10091244 DB - PRIME DP - Unbound Medicine ER -