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Silence of lncRNA UCA1 Represses the Growth and Tube Formation of Human Microvascular Endothelial Cells Through miR-195.
Cell Physiol Biochem 2018; 49(4):1499-1511CP

Abstract

BACKGROUND/AIMS

Recent studies have suggested that several lncRNAs contribute to the angiogenic function of endothelial cells. Herein, we set out to reveal whether lncRNA UCA1 has functions in endothelial angiogenesis.

METHODS

The expression levels of lncRNA UCA1, miR-195 and CCND1 in human microvascular endothelial HMEC-1 cells were altered by transfection. Subsequently, cell viability, migration, tube formation and apoptosis of HMEC-1 cells were respectively assessed. The cross-talk between lncRNA UCA1, miR-195, CCND1, and MEK/ERK and mTOR signaling pathways were investigated by performing qRT-PCR and Western blotting.

RESULTS

Silence of lncRNA UCA1 repressed HMEC-1 cells viability, migration, tube formation, and induced apoptosis. Meanwhile, silence of lncRNA UCA1 significantly up-regulated miR-195 expression. These alterations induced by lncRNA UCA1 were further enhanced by miR-195 overexpression, while were attenuated by miR-195 suppression. Moreover, silence of lncRNA UCA1 deactivated MEK/ERK and mTOR signaling pathways via a miR-195-dependent regulation. And the deactivation of MEK/ERK and mTOR signaling pathways led to a down-regulation of CCND1.

CONCLUSION

This study demonstrates that silence of lncRNA UCA1 largely represses microvascular endothelial cells growth and tube formation. Silence of lncRNA UCA1 exerts its function possibly via up-regulation of miR-195, which in turn inactivates MEK/ERK and mTOR signaling pathways, and ultimately represses CCND1 expression.

Authors+Show Affiliations

Department of Vascular Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.The 4th Department of Surgery, Dongfang Hospital of Beijing University of Chinese Medicine, Beijing, China.Department of Vascular Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30205377

Citation

Yin, Dexin, et al. "Silence of lncRNA UCA1 Represses the Growth and Tube Formation of Human Microvascular Endothelial Cells Through MiR-195." Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, vol. 49, no. 4, 2018, pp. 1499-1511.
Yin D, Fu C, Sun D. Silence of lncRNA UCA1 Represses the Growth and Tube Formation of Human Microvascular Endothelial Cells Through miR-195. Cell Physiol Biochem. 2018;49(4):1499-1511.
Yin, D., Fu, C., & Sun, D. (2018). Silence of lncRNA UCA1 Represses the Growth and Tube Formation of Human Microvascular Endothelial Cells Through miR-195. Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 49(4), pp. 1499-1511. doi:10.1159/000493454.
Yin D, Fu C, Sun D. Silence of lncRNA UCA1 Represses the Growth and Tube Formation of Human Microvascular Endothelial Cells Through MiR-195. Cell Physiol Biochem. 2018;49(4):1499-1511. PubMed PMID: 30205377.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silence of lncRNA UCA1 Represses the Growth and Tube Formation of Human Microvascular Endothelial Cells Through miR-195. AU - Yin,Dexin, AU - Fu,Changgeng, AU - Sun,Dajun, Y1 - 2018/09/11/ PY - 2018/01/29/received PY - 2018/09/04/accepted PY - 2018/9/12/pubmed PY - 2018/10/17/medline PY - 2018/9/12/entrez KW - Angiogenesis KW - Atherosclerosis KW - CCND1 KW - lncRNA UCA1 KW - miR-195 SP - 1499 EP - 1511 JF - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology JO - Cell. Physiol. Biochem. VL - 49 IS - 4 N2 - BACKGROUND/AIMS: Recent studies have suggested that several lncRNAs contribute to the angiogenic function of endothelial cells. Herein, we set out to reveal whether lncRNA UCA1 has functions in endothelial angiogenesis. METHODS: The expression levels of lncRNA UCA1, miR-195 and CCND1 in human microvascular endothelial HMEC-1 cells were altered by transfection. Subsequently, cell viability, migration, tube formation and apoptosis of HMEC-1 cells were respectively assessed. The cross-talk between lncRNA UCA1, miR-195, CCND1, and MEK/ERK and mTOR signaling pathways were investigated by performing qRT-PCR and Western blotting. RESULTS: Silence of lncRNA UCA1 repressed HMEC-1 cells viability, migration, tube formation, and induced apoptosis. Meanwhile, silence of lncRNA UCA1 significantly up-regulated miR-195 expression. These alterations induced by lncRNA UCA1 were further enhanced by miR-195 overexpression, while were attenuated by miR-195 suppression. Moreover, silence of lncRNA UCA1 deactivated MEK/ERK and mTOR signaling pathways via a miR-195-dependent regulation. And the deactivation of MEK/ERK and mTOR signaling pathways led to a down-regulation of CCND1. CONCLUSION: This study demonstrates that silence of lncRNA UCA1 largely represses microvascular endothelial cells growth and tube formation. Silence of lncRNA UCA1 exerts its function possibly via up-regulation of miR-195, which in turn inactivates MEK/ERK and mTOR signaling pathways, and ultimately represses CCND1 expression. SN - 1421-9778 UR - https://www.unboundmedicine.com/medline/citation/30205377/Silence_of_lncRNA_UCA1_Represses_the_Growth_and_Tube_Formation_of_Human_Microvascular_Endothelial_Cells_Through_miR_195_ L2 - https://www.karger.com?DOI=10.1159/000493454 DB - PRIME DP - Unbound Medicine ER -