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Prevalence and Genetic Diversity of Grabloviruses in Free-Living Vitis spp.
Plant Dis. 2018 11; 102(11):2308-2316.PD

Abstract

The distribution and diversity of grapevine red blotch virus (GRBV) and wild Vitis virus 1 (WVV1) (genus Grablovirus; family Geminiviridae) were determined in free-living Vitis spp. in northern California and New York from 2013 to 2017. Grabloviruses were detected by polymerase chain reaction in 28% (57 of 203) of samples from California but in none of the 163 samples from New York. The incidence of GRBV in free-living vines was significantly higher in samples from California counties with high compared with low grape production (χ2 = 83.09; P < 0.001), and in samples near (<5 km) to compared with far (>5 km) from vineyards (χ2 = 57.58; P < 0.001). These results suggested a directional spread of GRBV inoculum predominantly from vineyards to free-living Vitis spp. WVV1 incidence was also significantly higher in areas with higher grape production acreage (χ2 = 16.02; P < 0.001). However, in contrast to GRBV, no differential distribution of WVV1 incidence was observed with regard to distance from vineyards (χ2 = 0.88; P = 0.3513). Two distinct phylogenetic clades were identified for both GRBV and WVV1 isolates from free-living Vitis spp., although the nucleotide sequence variability of the genomic diversity fragment was higher for WWV1 (94.3 to 99.8% sequence identity within clade 1 isolates and 90.1 to 100% within clade 2 isolates) than GRBV (98.3% between clade 1 isolates and 96.9 to 100% within clade 2 isolates). Additionally, evidence for intraspecific recombination events was found in WVV1 isolates and confirmed in GRBV isolates. The prevalence of grabloviruses in California free-living vines highlights the need for vigilance regarding potential grablovirus inoculum sources in order to protect new vineyard plantings and foundation stock vineyards in California.

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Authors+Show Affiliations

Cieniewicz E
Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456.
Thompson JR
Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853.
McLane H
Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853.
Perry KL
Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853.
Dangl GS
Foundation Plant Services, University of California, Davis 95616.
Corbett Q
Finger Lakes Community College, Canandaigua, NY 14424.
Martinson T
Section of Horticulture, School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456.
Wise A
Cornell Cooperative Extension, Long Island Horticultural Research and Extension Center, Riverhead, NY 11901.
Wallis A
Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station and (formerly) Cornell Cooperative Extension, Eastern New York Commercial Horticulture, Plattsburg, NY 12901.
O'Connell J
Cornell Cooperative Extension, Eastern New York Commercial Horticulture, Highland, NY 12528.
Dunst R
Double A Vineyards, Fredonia, NY 14063.
Cox K
Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station.
Fuchs M
Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station.

MeSH

CaliforniaFarmsGeminiviridaeGenetic VariationGeographyNew YorkPhylogenyPlant DiseasesVitis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

30207510

Citation

Cieniewicz, Elizabeth, et al. "Prevalence and Genetic Diversity of Grabloviruses in Free-Living Vitis Spp." Plant Disease, vol. 102, no. 11, 2018, pp. 2308-2316.
Cieniewicz E, Thompson JR, McLane H, et al. Prevalence and Genetic Diversity of Grabloviruses in Free-Living Vitis spp. Plant Dis. 2018;102(11):2308-2316.
Cieniewicz, E., Thompson, J. R., McLane, H., Perry, K. L., Dangl, G. S., Corbett, Q., Martinson, T., Wise, A., Wallis, A., O'Connell, J., Dunst, R., Cox, K., & Fuchs, M. (2018). Prevalence and Genetic Diversity of Grabloviruses in Free-Living Vitis spp. Plant Disease, 102(11), 2308-2316. https://doi.org/10.1094/PDIS-03-18-0496-RE
Cieniewicz E, et al. Prevalence and Genetic Diversity of Grabloviruses in Free-Living Vitis Spp. Plant Dis. 2018;102(11):2308-2316. PubMed PMID: 30207510.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence and Genetic Diversity of Grabloviruses in Free-Living Vitis spp. AU - Cieniewicz,Elizabeth, AU - Thompson,Jeremy R, AU - McLane,Heather, AU - Perry,Keith L, AU - Dangl,Gerald S, AU - Corbett,Quinlan, AU - Martinson,Timothy, AU - Wise,Alice, AU - Wallis,Anna, AU - O'Connell,James, AU - Dunst,Rick, AU - Cox,Kerik, AU - Fuchs,Marc, Y1 - 2018/09/12/ PY - 2018/9/13/pubmed PY - 2019/2/23/medline PY - 2018/9/13/entrez SP - 2308 EP - 2316 JF - Plant disease JO - Plant Dis VL - 102 IS - 11 N2 - The distribution and diversity of grapevine red blotch virus (GRBV) and wild Vitis virus 1 (WVV1) (genus Grablovirus; family Geminiviridae) were determined in free-living Vitis spp. in northern California and New York from 2013 to 2017. Grabloviruses were detected by polymerase chain reaction in 28% (57 of 203) of samples from California but in none of the 163 samples from New York. The incidence of GRBV in free-living vines was significantly higher in samples from California counties with high compared with low grape production (χ2 = 83.09; P < 0.001), and in samples near (<5 km) to compared with far (>5 km) from vineyards (χ2 = 57.58; P < 0.001). These results suggested a directional spread of GRBV inoculum predominantly from vineyards to free-living Vitis spp. WVV1 incidence was also significantly higher in areas with higher grape production acreage (χ2 = 16.02; P < 0.001). However, in contrast to GRBV, no differential distribution of WVV1 incidence was observed with regard to distance from vineyards (χ2 = 0.88; P = 0.3513). Two distinct phylogenetic clades were identified for both GRBV and WVV1 isolates from free-living Vitis spp., although the nucleotide sequence variability of the genomic diversity fragment was higher for WWV1 (94.3 to 99.8% sequence identity within clade 1 isolates and 90.1 to 100% within clade 2 isolates) than GRBV (98.3% between clade 1 isolates and 96.9 to 100% within clade 2 isolates). Additionally, evidence for intraspecific recombination events was found in WVV1 isolates and confirmed in GRBV isolates. The prevalence of grabloviruses in California free-living vines highlights the need for vigilance regarding potential grablovirus inoculum sources in order to protect new vineyard plantings and foundation stock vineyards in California. SN - 0191-2917 UR - https://www.unboundmedicine.com/medline/citation/30207510/Prevalence_and_Genetic_Diversity_of_Grabloviruses_in_Free_Living_Vitis_spp_ DB - PRIME DP - Unbound Medicine ER -