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Antimicrobial resistance, virulence gene profile and molecular typing of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, northwest China.
J Glob Antimicrob Resist. 2019 03; 16:98-104.JG

Abstract

OBJECTIVES

Staphylococcus aureus (SA) is a major pathogen causing dairy cow mastitis and endometritis. Recently, animal-derived SA strains pose a serious public-health threat. However, little is known about antimicrobial resistance and virulence factors of SA isolated from dairy cows in Xinjiang, China. In this study, antimicrobial resistance, virulence gene profiles and genotypes of SA from clinical mastitis and endometritis in dairy cows were investigated.

METHODS

A total of 337 clinical samples (186 milk samples from clinical mastitis cases and 151 endometritis swab samples) were collected from 15 large-scale dairy farms and were screened for SA. All SA isolates were subjected to antimicrobial susceptibility testing, detection of virulence genes and molecular typing.

RESULTS

A total of 155 SA strains were isolated; 22 (14.2%) were methicillin-resistant S. aureus (MRSA). Resistance of MRSA isolates was significantly higher than that of methicillin-susceptible S. aureus (MSSA). The percentage of virulence genes varied between MSSA and MRSA. The strains could be divided into two SCCmec types (I and IVa), three agr types (I, II and III) and four spa types (t779, t2883, t13751 and t1939). MLST identified 14 sequence types, among which ST1 and ST9 had relatively high detection rates.

CONCLUSIONS

These findings revealed that ST9-t1939-agrI was the main genotype of MSSA, whilst ST1-SCCmecI-t1939-agrI was the main genotype of MRSA from dairy cows. More significantly, a novel ST (STX) was identified for the first time. The majority of SA strains from dairy cows were multidrug-resistant and carried multiple virulence genes, posing a potential public-health risk.

Authors+Show Affiliations

College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China. Electronic address: qj710625@163.com.College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.College of Animal Science and Technology, Shihezi University, North Street No. 4, Shihezi, Xinjiang 832003, China.Center for Animal Disease Prevention and Control, Urumqi, Xinjiang 830000, China.Center for Animal Disease Prevention and Control, Tacheng, Xinjiang 834700, China.Center for Animal Disease Prevention and Control, Tacheng, Xinjiang 834700, China.Center for Animal Disease Prevention and Control, Changji, Xinjiang 831100, China.State Key Lab of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30213718

Citation

Dan, Meng, et al. "Antimicrobial Resistance, Virulence Gene Profile and Molecular Typing of Staphylococcus Aureus Isolates From Dairy Cows in Xinjiang Province, Northwest China." Journal of Global Antimicrobial Resistance, vol. 16, 2019, pp. 98-104.
Dan M, Yehui W, Qingling M, et al. Antimicrobial resistance, virulence gene profile and molecular typing of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, northwest China. J Glob Antimicrob Resist. 2019;16:98-104.
Dan, M., Yehui, W., Qingling, M., Jun, Q., Xingxing, Z., Shuai, M., Kuojun, C., Jinsheng, Z., Zibing, C., Zaichao, Z., & Xuepeng, C. (2019). Antimicrobial resistance, virulence gene profile and molecular typing of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, northwest China. Journal of Global Antimicrobial Resistance, 16, 98-104. https://doi.org/10.1016/j.jgar.2018.08.024
Dan M, et al. Antimicrobial Resistance, Virulence Gene Profile and Molecular Typing of Staphylococcus Aureus Isolates From Dairy Cows in Xinjiang Province, Northwest China. J Glob Antimicrob Resist. 2019;16:98-104. PubMed PMID: 30213718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial resistance, virulence gene profile and molecular typing of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, northwest China. AU - Dan,Meng, AU - Yehui,Wu, AU - Qingling,Meng, AU - Jun,Qiao, AU - Xingxing,Zhang, AU - Shuai,Ma, AU - Kuojun,Cai, AU - Jinsheng,Zhang, AU - Zibing,Cheng, AU - Zaichao,Zhang, AU - Xuepeng,Cai, Y1 - 2018/09/10/ PY - 2017/10/06/received PY - 2018/08/30/revised PY - 2018/08/31/accepted PY - 2018/9/15/pubmed PY - 2020/3/26/medline PY - 2018/9/15/entrez KW - Antimicrobial resistance KW - Dairy cows KW - Molecular typing KW - Staphylococcus aureus KW - Virulence gene SP - 98 EP - 104 JF - Journal of global antimicrobial resistance JO - J Glob Antimicrob Resist VL - 16 N2 - OBJECTIVES: Staphylococcus aureus (SA) is a major pathogen causing dairy cow mastitis and endometritis. Recently, animal-derived SA strains pose a serious public-health threat. However, little is known about antimicrobial resistance and virulence factors of SA isolated from dairy cows in Xinjiang, China. In this study, antimicrobial resistance, virulence gene profiles and genotypes of SA from clinical mastitis and endometritis in dairy cows were investigated. METHODS: A total of 337 clinical samples (186 milk samples from clinical mastitis cases and 151 endometritis swab samples) were collected from 15 large-scale dairy farms and were screened for SA. All SA isolates were subjected to antimicrobial susceptibility testing, detection of virulence genes and molecular typing. RESULTS: A total of 155 SA strains were isolated; 22 (14.2%) were methicillin-resistant S. aureus (MRSA). Resistance of MRSA isolates was significantly higher than that of methicillin-susceptible S. aureus (MSSA). The percentage of virulence genes varied between MSSA and MRSA. The strains could be divided into two SCCmec types (I and IVa), three agr types (I, II and III) and four spa types (t779, t2883, t13751 and t1939). MLST identified 14 sequence types, among which ST1 and ST9 had relatively high detection rates. CONCLUSIONS: These findings revealed that ST9-t1939-agrI was the main genotype of MSSA, whilst ST1-SCCmecI-t1939-agrI was the main genotype of MRSA from dairy cows. More significantly, a novel ST (STX) was identified for the first time. The majority of SA strains from dairy cows were multidrug-resistant and carried multiple virulence genes, posing a potential public-health risk. SN - 2213-7173 UR - https://www.unboundmedicine.com/medline/citation/30213718/Antimicrobial_resistance_virulence_gene_profile_and_molecular_typing_of_Staphylococcus_aureus_isolates_from_dairy_cows_in_Xinjiang_Province_northwest_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2213-7165(18)30174-7 DB - PRIME DP - Unbound Medicine ER -