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Electrochemical sandwich immunoassay for Escherichia coli O157:H7 based on the use of magnetic nanoparticles and graphene functionalized with electrocatalytically active Au@Pt core/shell nanoparticles.
Mikrochim Acta. 2018 09 13; 185(10):455.MA

Abstract

A highly sensitive electrochemical sandwich immunoassay is described for determination of Escherichia coli O157:H7 (E. coli O157:H7). Silica coated magnetite nanoparticles (Fe3O4) were modified with primary antibody to capture E. coli O157:H7. Gold-platinum core/shell nanoparticles (Au@Pt NPs) with different Pt shell thicknesses were prepared via changing the molar ratio of H2PtCl6 to HAuCl4 in the precursor solution. The optimized Au@Pt NPs exhibit enhanced activity in the electrocatalytic reduction of hydrogen peroxide (H2O2). The Au@Pt NPs were modified with graphene that was functionalized with Neutral Red, and then used as an electrochemical label for secondary antibodies and horseradish peroxidase (HRP). The sandwich immunocomplexes were magnetically absorbed on a 4-channel screen printed carbon electrode. Due to the catalysis of the Au@Pt NPs and HRP, the signal is strongly amplified in the presence of H2O2 when using thionine as the electron mediator. Under optimal conditions, the immunoassay has a linear response in the 4.0 × 102 to 4.0 × 108 CFU·mL-1 concentration range, with a limit of detection of 91 CFU·mL-1 (at an S/N ratio of 3). Graphical abstract Preparation of Au@Pt core/shell nanoparticles with different Pt shell thickness (A), rGO-NR (B), rGO-NR-Au@Pt-Ab2-HRP (C) and the preparation and the detection process of the immunoassay (D). rGO: reduced graphene oxide, GO: graphene oxide, NR: Neutral Red, HRP: horseradish peroxidase, AuNPs: gold nanoparticles, Fe3O4@SiO2: Silica coated magnetite nanoparticles, 4-SPCE: 4-channel screen printed carbon electrode.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zhu F
Food Safety Key Laboratory of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, China.
Zhao G
Food Safety Key Laboratory of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, China.
Dou W
Food Safety Key Laboratory of Zhejiang Province, College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, China. wdou@zjsu.edu.cn.

MeSH

CatalysisElectrochemistryEscherichia coli O157GoldGraphiteHydrogen PeroxideImmunoassayMagnetite NanoparticlesMetal NanoparticlesModels, MolecularMolecular ConformationOxidation-ReductionPhenothiazinesPlatinum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30215173

Citation

Zhu, Fanjun, et al. "Electrochemical Sandwich Immunoassay for Escherichia Coli O157:H7 Based On the Use of Magnetic Nanoparticles and Graphene Functionalized With Electrocatalytically Active Au@Pt Core/shell Nanoparticles." Mikrochimica Acta, vol. 185, no. 10, 2018, p. 455.
Zhu F, Zhao G, Dou W. Electrochemical sandwich immunoassay for Escherichia coli O157:H7 based on the use of magnetic nanoparticles and graphene functionalized with electrocatalytically active Au@Pt core/shell nanoparticles. Mikrochim Acta. 2018;185(10):455.
Zhu, F., Zhao, G., & Dou, W. (2018). Electrochemical sandwich immunoassay for Escherichia coli O157:H7 based on the use of magnetic nanoparticles and graphene functionalized with electrocatalytically active Au@Pt core/shell nanoparticles. Mikrochimica Acta, 185(10), 455. https://doi.org/10.1007/s00604-018-2984-2
Zhu F, Zhao G, Dou W. Electrochemical Sandwich Immunoassay for Escherichia Coli O157:H7 Based On the Use of Magnetic Nanoparticles and Graphene Functionalized With Electrocatalytically Active Au@Pt Core/shell Nanoparticles. Mikrochim Acta. 2018 09 13;185(10):455. PubMed PMID: 30215173.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical sandwich immunoassay for Escherichia coli O157:H7 based on the use of magnetic nanoparticles and graphene functionalized with electrocatalytically active Au@Pt core/shell nanoparticles. AU - Zhu,Fanjun, AU - Zhao,Guangying, AU - Dou,Wenchao, Y1 - 2018/09/13/ PY - 2018/05/19/received PY - 2018/08/29/accepted PY - 2018/9/15/entrez PY - 2018/9/15/pubmed PY - 2019/4/2/medline KW - Bacteria KW - Bimetallic nanoparticles KW - Food safety KW - Gold-platinum core/shell nanoparticles KW - Horseradish peroxidase KW - Hydrogen peroxide KW - Nanomaterial KW - Reduced graphene oxide KW - Screen printed carbon electrode KW - Signal amplification SP - 455 EP - 455 JF - Mikrochimica acta JO - Mikrochim Acta VL - 185 IS - 10 N2 - A highly sensitive electrochemical sandwich immunoassay is described for determination of Escherichia coli O157:H7 (E. coli O157:H7). Silica coated magnetite nanoparticles (Fe3O4) were modified with primary antibody to capture E. coli O157:H7. Gold-platinum core/shell nanoparticles (Au@Pt NPs) with different Pt shell thicknesses were prepared via changing the molar ratio of H2PtCl6 to HAuCl4 in the precursor solution. The optimized Au@Pt NPs exhibit enhanced activity in the electrocatalytic reduction of hydrogen peroxide (H2O2). The Au@Pt NPs were modified with graphene that was functionalized with Neutral Red, and then used as an electrochemical label for secondary antibodies and horseradish peroxidase (HRP). The sandwich immunocomplexes were magnetically absorbed on a 4-channel screen printed carbon electrode. Due to the catalysis of the Au@Pt NPs and HRP, the signal is strongly amplified in the presence of H2O2 when using thionine as the electron mediator. Under optimal conditions, the immunoassay has a linear response in the 4.0 × 102 to 4.0 × 108 CFU·mL-1 concentration range, with a limit of detection of 91 CFU·mL-1 (at an S/N ratio of 3). Graphical abstract Preparation of Au@Pt core/shell nanoparticles with different Pt shell thickness (A), rGO-NR (B), rGO-NR-Au@Pt-Ab2-HRP (C) and the preparation and the detection process of the immunoassay (D). rGO: reduced graphene oxide, GO: graphene oxide, NR: Neutral Red, HRP: horseradish peroxidase, AuNPs: gold nanoparticles, Fe3O4@SiO2: Silica coated magnetite nanoparticles, 4-SPCE: 4-channel screen printed carbon electrode. SN - 1436-5073 UR - https://www.unboundmedicine.com/medline/citation/30215173/Electrochemical_sandwich_immunoassay_for_Escherichia_coli_O157:H7_based_on_the_use_of_magnetic_nanoparticles_and_graphene_functionalized_with_electrocatalytically_active_Au@Pt_core/shell_nanoparticles_ DB - PRIME DP - Unbound Medicine ER -