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[Molecular cloning and functional analysis of DNA regions of plasmid RP4 determining the incompatibility properties].
Genetika. 1986 Aug; 22(8):2025-34.G

Abstract

Sau3A-generated DNA fragments determining incompatibility functions of the plasmid RP4 were cloned on the vectors pTK16 and pBR322. Inc+ recombinant plasmids were divided into two types: 1) expressing incompatibility only towards the homologous RP4 replicon, 2) expressing incompatibility - both towards the homologous RP4 replicon and towards the heterologous replicons of plasmids R906 and R751. For one member of the first type plasmids it was shown that the cloned Inc+-specific insertion derived from the region of location of the EcoRI restriction site. The majority of the Inc+ recombinant plasmids showed asymmetric expression of incompatibility, predominantly eliminating the resident IncP plasmid.

Authors

Riabchenko LE
No affiliation info available
Riabchenko NF
No affiliation info available
Dobrovol'skiĭ P
No affiliation info available
Sakanian VA
No affiliation info available
Alikhanian SI
No affiliation info available

MeSH

Chromosome MappingCloning, MolecularDNA Restriction EnzymesDNA, BacterialDNA, RecombinantEscherichia coliPlasmidsRepliconTransformation, Bacterial

Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

3021575

Citation

Riabchenko, L E., et al. "[Molecular Cloning and Functional Analysis of DNA Regions of Plasmid RP4 Determining the Incompatibility Properties]." Genetika, vol. 22, no. 8, 1986, pp. 2025-34.
Riabchenko LE, Riabchenko NF, Dobrovol'skiĭ P, et al. [Molecular cloning and functional analysis of DNA regions of plasmid RP4 determining the incompatibility properties]. Genetika. 1986;22(8):2025-34.
Riabchenko, L. E., Riabchenko, N. F., Dobrovol'skiĭ, P., Sakanian, V. A., & Alikhanian, S. I. (1986). [Molecular cloning and functional analysis of DNA regions of plasmid RP4 determining the incompatibility properties]. Genetika, 22(8), 2025-34.
Riabchenko LE, et al. [Molecular Cloning and Functional Analysis of DNA Regions of Plasmid RP4 Determining the Incompatibility Properties]. Genetika. 1986;22(8):2025-34. PubMed PMID: 3021575.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Molecular cloning and functional analysis of DNA regions of plasmid RP4 determining the incompatibility properties]. AU - Riabchenko,L E, AU - Riabchenko,N F, AU - Dobrovol'skiĭ,P, AU - Sakanian,V A, AU - Alikhanian,S I, PY - 1986/8/1/pubmed PY - 1986/8/1/medline PY - 1986/8/1/entrez SP - 2025 EP - 34 JF - Genetika JO - Genetika VL - 22 IS - 8 N2 - Sau3A-generated DNA fragments determining incompatibility functions of the plasmid RP4 were cloned on the vectors pTK16 and pBR322. Inc+ recombinant plasmids were divided into two types: 1) expressing incompatibility only towards the homologous RP4 replicon, 2) expressing incompatibility - both towards the homologous RP4 replicon and towards the heterologous replicons of plasmids R906 and R751. For one member of the first type plasmids it was shown that the cloned Inc+-specific insertion derived from the region of location of the EcoRI restriction site. The majority of the Inc+ recombinant plasmids showed asymmetric expression of incompatibility, predominantly eliminating the resident IncP plasmid. SN - 0016-6758 UR - https://www.unboundmedicine.com/medline/citation/3021575/[Molecular_cloning_and_functional_analysis_of_DNA_regions_of_plasmid_RP4_determining_the_incompatibility_properties]_ DB - PRIME DP - Unbound Medicine ER -