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The Relative Occurrence of Grapevine leafroll-associated virus 3 and Grapevine red blotch virus in Washington State Vineyards.
Plant Dis. 2018 11; 102(11):2129-2135.PD

Abstract

Vineyard surveys were conducted for three consecutive seasons in eastern Washington State, the major grapevine-growing region in the state, to document the occurrence of Grapevine leafroll-associated virus 3 (GLRaV-3) and Grapevine red blotch virus (GRBV). The majority of samples were collected from red-berried wine grape (Vitis vinifera) cultivars exhibiting symptoms of or suspected for grapevine leafroll (GLD) and red blotch (GRBD) diseases. A limited number of samples from white-berried cultivars were collected randomly due to the lack of visual symptoms. Samples were collected from a total of 2,063 grapevines from 18 red-berried cultivars and seven white-berried cultivars planted in eight American Viticultural Areas and tested for GLRaV-3 and GRBV using RT-PCR and PCR, respectively. The results showed 67.77% and 6.01% of total samples positive for GLRaV-3 and GRBV, respectively, and 9.06% of samples positive for both viruses. About 17% of samples tested negative for the two viruses, but some of these samples were positive for GLRaV-2 and GLRaV-4. Overall results indicated that GLRaV-3 was more common than GRBV, independent of cultivars and the geographic origin of samples. Due to variability in symptoms in red-berried cultivars, virus-specific diagnostic assays were deemed necessary for reliable identification of GLRaV-3 and GRBV and to differentiate GLD and GRBD symptoms from those induced by biotic and abiotic stresses in vineyards. A multiplex PCR protocol was developed for simultaneous detection of GLRaV-3 and GRBV in grapevine samples. A global phylogenetic analysis of GRBV genome sequences revealed segregation of virus isolates from Washington State vineyards into two distinct clades, with the majority of isolates belonging to clade II.

Authors+Show Affiliations

Department of Plant Pathology, Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA 99350.Department of Agriculture, Columbia Basin College, Pasco, WA 99301.Department of Plant Pathology, Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA 99350.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30226418

Citation

Adiputra, Jati, et al. "The Relative Occurrence of Grapevine Leafroll-associated Virus 3 and Grapevine Red Blotch Virus in Washington State Vineyards." Plant Disease, vol. 102, no. 11, 2018, pp. 2129-2135.
Adiputra J, Kesoju SR, Naidu RA. The Relative Occurrence of Grapevine leafroll-associated virus 3 and Grapevine red blotch virus in Washington State Vineyards. Plant Dis. 2018;102(11):2129-2135.
Adiputra, J., Kesoju, S. R., & Naidu, R. A. (2018). The Relative Occurrence of Grapevine leafroll-associated virus 3 and Grapevine red blotch virus in Washington State Vineyards. Plant Disease, 102(11), 2129-2135. https://doi.org/10.1094/PDIS-12-17-1962-RE
Adiputra J, Kesoju SR, Naidu RA. The Relative Occurrence of Grapevine Leafroll-associated Virus 3 and Grapevine Red Blotch Virus in Washington State Vineyards. Plant Dis. 2018;102(11):2129-2135. PubMed PMID: 30226418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Relative Occurrence of Grapevine leafroll-associated virus 3 and Grapevine red blotch virus in Washington State Vineyards. AU - Adiputra,Jati, AU - Kesoju,Sandya R, AU - Naidu,Rayapati A, Y1 - 2018/09/17/ PY - 2018/9/19/pubmed PY - 2019/2/23/medline PY - 2018/9/19/entrez SP - 2129 EP - 2135 JF - Plant disease JO - Plant Dis VL - 102 IS - 11 N2 - Vineyard surveys were conducted for three consecutive seasons in eastern Washington State, the major grapevine-growing region in the state, to document the occurrence of Grapevine leafroll-associated virus 3 (GLRaV-3) and Grapevine red blotch virus (GRBV). The majority of samples were collected from red-berried wine grape (Vitis vinifera) cultivars exhibiting symptoms of or suspected for grapevine leafroll (GLD) and red blotch (GRBD) diseases. A limited number of samples from white-berried cultivars were collected randomly due to the lack of visual symptoms. Samples were collected from a total of 2,063 grapevines from 18 red-berried cultivars and seven white-berried cultivars planted in eight American Viticultural Areas and tested for GLRaV-3 and GRBV using RT-PCR and PCR, respectively. The results showed 67.77% and 6.01% of total samples positive for GLRaV-3 and GRBV, respectively, and 9.06% of samples positive for both viruses. About 17% of samples tested negative for the two viruses, but some of these samples were positive for GLRaV-2 and GLRaV-4. Overall results indicated that GLRaV-3 was more common than GRBV, independent of cultivars and the geographic origin of samples. Due to variability in symptoms in red-berried cultivars, virus-specific diagnostic assays were deemed necessary for reliable identification of GLRaV-3 and GRBV and to differentiate GLD and GRBD symptoms from those induced by biotic and abiotic stresses in vineyards. A multiplex PCR protocol was developed for simultaneous detection of GLRaV-3 and GRBV in grapevine samples. A global phylogenetic analysis of GRBV genome sequences revealed segregation of virus isolates from Washington State vineyards into two distinct clades, with the majority of isolates belonging to clade II. SN - 0191-2917 UR - https://www.unboundmedicine.com/medline/citation/30226418/The_Relative_Occurrence_of_Grapevine_leafroll_associated_virus_3_and_Grapevine_red_blotch_virus_in_Washington_State_Vineyards_ L2 - https://apsjournals.apsnet.org/doi/10.1094/PDIS-12-17-1962-RE?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -