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The Distribution and Detection of Grapevine red blotch virus in its Host Depend on Time of Sampling and Tissue Type.
Plant Dis. 2018 11; 102(11):2187-2193.PD

Abstract

Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch, an emerging disease that affects cultivated grapevine such as Vitis vinifera. The ability to detect viruses in grapevine is often hindered by low virus titers compounded by a variable distribution in the plant and seasonal variations. In order to examine these two variables in relation to GRBV, we developed a quantitative polymerase chain reaction (qPCR) method that incorporates both internal and external references to enhance assay robustness. In greenhouse-grown vines infected with GRBV, qPCR identified highest virus titers in the petioles of fully expanded leaves and significantly reduced levels of virus in the shoot extremities. In vineyard-grown vines infected with GRBV, the virus titer in July and October 2016 followed a pattern similar to that found for the greenhouse-grown plants but, most strikingly, close to half (44%) of the samples analyzed in June 2015 tested negative for infection. The technique presented and results obtained highlight the variability of virus distribution in its host and provide a useful guide for selecting the best tissues for optimal GRBV diagnosis.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Setiono FJ
Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
Chatterjee D
Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, 14853, USA.
Fuchs M
Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, New York State Agricultural Experiment Station, Geneva 14456, USA.
Perry KL
Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University.
Thompson JR
Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University.

MeSH

GeminiviridaeOrgan SpecificityPlant DiseasesPlant LeavesTime FactorsVitis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

30226420

Citation

Setiono, Felicia J., et al. "The Distribution and Detection of Grapevine Red Blotch Virus in Its Host Depend On Time of Sampling and Tissue Type." Plant Disease, vol. 102, no. 11, 2018, pp. 2187-2193.
Setiono FJ, Chatterjee D, Fuchs M, et al. The Distribution and Detection of Grapevine red blotch virus in its Host Depend on Time of Sampling and Tissue Type. Plant Dis. 2018;102(11):2187-2193.
Setiono, F. J., Chatterjee, D., Fuchs, M., Perry, K. L., & Thompson, J. R. (2018). The Distribution and Detection of Grapevine red blotch virus in its Host Depend on Time of Sampling and Tissue Type. Plant Disease, 102(11), 2187-2193. https://doi.org/10.1094/PDIS-03-18-0450-RE
Setiono FJ, et al. The Distribution and Detection of Grapevine Red Blotch Virus in Its Host Depend On Time of Sampling and Tissue Type. Plant Dis. 2018;102(11):2187-2193. PubMed PMID: 30226420.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Distribution and Detection of Grapevine red blotch virus in its Host Depend on Time of Sampling and Tissue Type. AU - Setiono,Felicia J, AU - Chatterjee,Debotri, AU - Fuchs,Marc, AU - Perry,Keith L, AU - Thompson,Jeremy R, Y1 - 2018/09/18/ PY - 2018/9/19/pubmed PY - 2019/2/23/medline PY - 2018/9/19/entrez SP - 2187 EP - 2193 JF - Plant disease JO - Plant Dis VL - 102 IS - 11 N2 - Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch, an emerging disease that affects cultivated grapevine such as Vitis vinifera. The ability to detect viruses in grapevine is often hindered by low virus titers compounded by a variable distribution in the plant and seasonal variations. In order to examine these two variables in relation to GRBV, we developed a quantitative polymerase chain reaction (qPCR) method that incorporates both internal and external references to enhance assay robustness. In greenhouse-grown vines infected with GRBV, qPCR identified highest virus titers in the petioles of fully expanded leaves and significantly reduced levels of virus in the shoot extremities. In vineyard-grown vines infected with GRBV, the virus titer in July and October 2016 followed a pattern similar to that found for the greenhouse-grown plants but, most strikingly, close to half (44%) of the samples analyzed in June 2015 tested negative for infection. The technique presented and results obtained highlight the variability of virus distribution in its host and provide a useful guide for selecting the best tissues for optimal GRBV diagnosis. SN - 0191-2917 UR - https://www.unboundmedicine.com/medline/citation/30226420/The_Distribution_and_Detection_of_Grapevine_red_blotch_virus_in_its_Host_Depend_on_Time_of_Sampling_and_Tissue_Type_ DB - PRIME DP - Unbound Medicine ER -