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Genotoxicity by long-term exposure to the auxinic herbicides 2,4-dichlorophenoxyacetic acid and dicamba on Cnesterodon decemmaculatus (Pisces: Poeciliidae).
Environ Pollut. 2018 Dec; 243(Pt A):670-678.EP

Abstract

Long-term genotoxic effects of two auxinic herbicide formulations, namely, the 58.4% 2,4-dichlorophenoxyacetic acid (2,4-D)-based DMA® and the 57.7% dicamba (DIC)-based Banvel® were evaluated on Cnesterodon decemmaculatus. Primary DNA lesions were analyzed by the single-cell gel electrophoresis methodology. Two sublethal concentrations were tested for each herbicide corresponding to 2.5% and 5% of the LC5096h values. Accordingly, fish were exposed to 25.2 and 50.4 mg/L or 41 and 82 mg/L for 2,4-D and DIC, respectively. Fish were continuously exposed for 28 days with replacement of test solutions every 3 days. Genotoxicity was evaluated in ten individuals from each experimental point at the beginning of the exposure period (0 day) and at 7, 14, 21 and 28 days thereafter. Results demonstrated for first time that 2,4-D-based formulation DMA® induced primary DNA strand breaks after 7-28 days exposure on C. decemmaculatus regardless its concentration. On the other hand, DIC-based formulation Banvel® exerted its genotoxic effect after exposure during 7-14 days and 7 days of 2.5 and 5% LC5096h, respectively. The present study represents the first evidence of primary DNA lesions induced by two widely employed auxinic herbicides on C. decemmaculatus, namely 2,4-D and DIC, following long-term exposure.

Authors+Show Affiliations

Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nº 3, B1904AMA, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nº 3, B1904AMA, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Calle 64 Nº 3, B1904AMA, La Plata, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: ssoloneski@yahoo.com.ar.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30228061

Citation

Ruiz de Arcaute, Celeste, et al. "Genotoxicity By Long-term Exposure to the Auxinic Herbicides 2,4-dichlorophenoxyacetic Acid and Dicamba On Cnesterodon Decemmaculatus (Pisces: Poeciliidae)." Environmental Pollution (Barking, Essex : 1987), vol. 243, no. Pt A, 2018, pp. 670-678.
Ruiz de Arcaute C, Larramendy ML, Soloneski S. Genotoxicity by long-term exposure to the auxinic herbicides 2,4-dichlorophenoxyacetic acid and dicamba on Cnesterodon decemmaculatus (Pisces: Poeciliidae). Environ Pollut. 2018;243(Pt A):670-678.
Ruiz de Arcaute, C., Larramendy, M. L., & Soloneski, S. (2018). Genotoxicity by long-term exposure to the auxinic herbicides 2,4-dichlorophenoxyacetic acid and dicamba on Cnesterodon decemmaculatus (Pisces: Poeciliidae). Environmental Pollution (Barking, Essex : 1987), 243(Pt A), 670-678. https://doi.org/10.1016/j.envpol.2018.09.040
Ruiz de Arcaute C, Larramendy ML, Soloneski S. Genotoxicity By Long-term Exposure to the Auxinic Herbicides 2,4-dichlorophenoxyacetic Acid and Dicamba On Cnesterodon Decemmaculatus (Pisces: Poeciliidae). Environ Pollut. 2018;243(Pt A):670-678. PubMed PMID: 30228061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxicity by long-term exposure to the auxinic herbicides 2,4-dichlorophenoxyacetic acid and dicamba on Cnesterodon decemmaculatus (Pisces: Poeciliidae). AU - Ruiz de Arcaute,Celeste, AU - Larramendy,Marcelo L, AU - Soloneski,Sonia, Y1 - 2018/09/08/ PY - 2018/05/21/received PY - 2018/08/09/revised PY - 2018/09/07/accepted PY - 2018/9/20/pubmed PY - 2018/12/12/medline PY - 2018/9/20/entrez KW - 2,4-D-based formulation DMA(®) KW - Dicamba-based formulation Banvel(®) KW - Long-term assay KW - SCGE assay KW - Sublethal effects SP - 670 EP - 678 JF - Environmental pollution (Barking, Essex : 1987) JO - Environ. Pollut. VL - 243 IS - Pt A N2 - Long-term genotoxic effects of two auxinic herbicide formulations, namely, the 58.4% 2,4-dichlorophenoxyacetic acid (2,4-D)-based DMA® and the 57.7% dicamba (DIC)-based Banvel® were evaluated on Cnesterodon decemmaculatus. Primary DNA lesions were analyzed by the single-cell gel electrophoresis methodology. Two sublethal concentrations were tested for each herbicide corresponding to 2.5% and 5% of the LC5096h values. Accordingly, fish were exposed to 25.2 and 50.4 mg/L or 41 and 82 mg/L for 2,4-D and DIC, respectively. Fish were continuously exposed for 28 days with replacement of test solutions every 3 days. Genotoxicity was evaluated in ten individuals from each experimental point at the beginning of the exposure period (0 day) and at 7, 14, 21 and 28 days thereafter. Results demonstrated for first time that 2,4-D-based formulation DMA® induced primary DNA strand breaks after 7-28 days exposure on C. decemmaculatus regardless its concentration. On the other hand, DIC-based formulation Banvel® exerted its genotoxic effect after exposure during 7-14 days and 7 days of 2.5 and 5% LC5096h, respectively. The present study represents the first evidence of primary DNA lesions induced by two widely employed auxinic herbicides on C. decemmaculatus, namely 2,4-D and DIC, following long-term exposure. SN - 1873-6424 UR - https://www.unboundmedicine.com/medline/citation/30228061/Genotoxicity_by_long_term_exposure_to_the_auxinic_herbicides_24_dichlorophenoxyacetic_acid_and_dicamba_on_Cnesterodon_decemmaculatus__Pisces:_Poeciliidae__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0269-7491(18)32267-X DB - PRIME DP - Unbound Medicine ER -