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Sharing of human milk oligosaccharides degradants within bifidobacterial communities in faecal cultures supplemented with Bifidobacterium bifidum.
Sci Rep. 2018 09 18; 8(1):13958.SR

Abstract

Gut microbiota of breast-fed infants are generally rich in bifidobacteria. Recent studies show that infant gut-associated bifidobacteria can assimilate human milk oligosaccharides (HMOs) specifically among the gut microbes. Nonetheless, little is known about how bifidobacterial-rich communities are shaped in the gut. Interestingly, HMOs assimilation ability is not related to the dominance of each species. Bifidobacterium longum susbp. longum and Bifidobacterium breve are commonly found as the dominant species in infant stools; however, they show limited HMOs assimilation ability in vitro. In contrast, avid in vitro HMOs consumers, Bifidobacterium bifidum and Bifidobacterium longum subsp. infantis, are less abundant in infant stools. In this study, we observed altruistic behaviour by B. bifidum when incubated in HMOs-containing faecal cultures. Four B. bifidum strains, all of which contained complete sets of HMO-degrading genes, commonly left HMOs degradants unconsumed during in vitro growth. These strains stimulated the growth of other Bifidobacterium species when added to faecal cultures supplemented with HMOs, thereby increasing the prevalence of bifidobacteria in faecal communities. Enhanced HMOs consumption by B. bifidum-supplemented cultures was also observed. We also determined the complete genome sequences of B. bifidum strains JCM7004 and TMC3115. Our results suggest B. bifidum-mediated cross-feeding of HMOs degradants within bifidobacterial communities.

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Authors+Show Affiliations

Gotoh A
Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
Katoh T
Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
Sakanaka M
Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan.
Ling Y
Graduate School of Medical and Dental Sciences, Niigata University, Chuo-ku, Niigata, 951-8510, Japan.
Yamada C
Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan.
Asakuma S
Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Sapporo, Hokkaido, 062-8555, Japan.
Urashima T
Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
Tomabechi Y
Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan.
Katayama-Ikegami A
Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan.
Kurihara S
Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan.
Yamamoto K
Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan.
Harata G
Takanashi Milk Products Co., Ltd., Yokohama, Kanagawa, 241-0023, Japan.
He F
Takanashi Milk Products Co., Ltd., Yokohama, Kanagawa, 241-0023, Japan.
Hirose J
School of Human Cultures, The University of Shiga Prefecture, Hikone, Shiga, 522-8533, Japan.
Kitaoka M
Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, 305-8642, Japan.
Okuda S
Graduate School of Medical and Dental Sciences, Niigata University, Chuo-ku, Niigata, 951-8510, Japan. okd@med.niigata-u.ac.jp.
Katayama T
Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan. takane@lif.kyoto-u.ac.jp. Faculty of Bioresources and Environmental Sciences, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan. takane@lif.kyoto-u.ac.jp.

MeSH

AdultBacterial ProteinsBifidobacteriales InfectionsBifidobacteriumCells, CulturedChild, PreschoolDietary SupplementsFecesFemaleGastrointestinal MicrobiomeGenome, BacterialHumansInfantMaleMilk, HumanOligosaccharides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30228375

Citation

Gotoh, Aina, et al. "Sharing of Human Milk Oligosaccharides Degradants Within Bifidobacterial Communities in Faecal Cultures Supplemented With Bifidobacterium Bifidum." Scientific Reports, vol. 8, no. 1, 2018, p. 13958.
Gotoh A, Katoh T, Sakanaka M, et al. Sharing of human milk oligosaccharides degradants within bifidobacterial communities in faecal cultures supplemented with Bifidobacterium bifidum. Sci Rep. 2018;8(1):13958.
Gotoh, A., Katoh, T., Sakanaka, M., Ling, Y., Yamada, C., Asakuma, S., Urashima, T., Tomabechi, Y., Katayama-Ikegami, A., Kurihara, S., Yamamoto, K., Harata, G., He, F., Hirose, J., Kitaoka, M., Okuda, S., & Katayama, T. (2018). Sharing of human milk oligosaccharides degradants within bifidobacterial communities in faecal cultures supplemented with Bifidobacterium bifidum. Scientific Reports, 8(1), 13958. https://doi.org/10.1038/s41598-018-32080-3
Gotoh A, et al. Sharing of Human Milk Oligosaccharides Degradants Within Bifidobacterial Communities in Faecal Cultures Supplemented With Bifidobacterium Bifidum. Sci Rep. 2018 09 18;8(1):13958. PubMed PMID: 30228375.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sharing of human milk oligosaccharides degradants within bifidobacterial communities in faecal cultures supplemented with Bifidobacterium bifidum. AU - Gotoh,Aina, AU - Katoh,Toshihiko, AU - Sakanaka,Mikiyasu, AU - Ling,Yiwei, AU - Yamada,Chihaya, AU - Asakuma,Sadaki, AU - Urashima,Tadasu, AU - Tomabechi,Yusuke, AU - Katayama-Ikegami,Ayako, AU - Kurihara,Shin, AU - Yamamoto,Kenji, AU - Harata,Gaku, AU - He,Fang, AU - Hirose,Junko, AU - Kitaoka,Motomitsu, AU - Okuda,Shujiro, AU - Katayama,Takane, Y1 - 2018/09/18/ PY - 2018/03/05/received PY - 2018/09/03/accepted PY - 2018/9/20/entrez PY - 2018/9/20/pubmed PY - 2019/11/15/medline SP - 13958 EP - 13958 JF - Scientific reports JO - Sci Rep VL - 8 IS - 1 N2 - Gut microbiota of breast-fed infants are generally rich in bifidobacteria. Recent studies show that infant gut-associated bifidobacteria can assimilate human milk oligosaccharides (HMOs) specifically among the gut microbes. Nonetheless, little is known about how bifidobacterial-rich communities are shaped in the gut. Interestingly, HMOs assimilation ability is not related to the dominance of each species. Bifidobacterium longum susbp. longum and Bifidobacterium breve are commonly found as the dominant species in infant stools; however, they show limited HMOs assimilation ability in vitro. In contrast, avid in vitro HMOs consumers, Bifidobacterium bifidum and Bifidobacterium longum subsp. infantis, are less abundant in infant stools. In this study, we observed altruistic behaviour by B. bifidum when incubated in HMOs-containing faecal cultures. Four B. bifidum strains, all of which contained complete sets of HMO-degrading genes, commonly left HMOs degradants unconsumed during in vitro growth. These strains stimulated the growth of other Bifidobacterium species when added to faecal cultures supplemented with HMOs, thereby increasing the prevalence of bifidobacteria in faecal communities. Enhanced HMOs consumption by B. bifidum-supplemented cultures was also observed. We also determined the complete genome sequences of B. bifidum strains JCM7004 and TMC3115. Our results suggest B. bifidum-mediated cross-feeding of HMOs degradants within bifidobacterial communities. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/30228375/Sharing_of_human_milk_oligosaccharides_degradants_within_bifidobacterial_communities_in_faecal_cultures_supplemented_with_Bifidobacterium_bifidum_ DB - PRIME DP - Unbound Medicine ER -