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Bioactive Composition, Antioxidant Activity, and Anticancer Potential of Freeze-Dried Extracts from Defatted Gac (Momordica cochinchinensis Spreng) Seeds.
Medicines (Basel). 2018 Sep 18; 5(3)M

Abstract

Background: Gac (Momordica cochinchinensis Spreng) seeds have long been used in traditional medicine as a remedy for numerous conditions due to a range of bioactive compounds. This study investigated the solvent extraction of compounds that could be responsible for antioxidant activity and anticancer potential. Methods: Defatted Gac seed kernel powder was extracted with different solvents: 100% water, 50% methanol:water, 70% ethanol:water, water saturated butanol, 100% methanol, and 100% ethanol. Trypsin inhibitors, saponins, phenolics, and antioxidant activity using the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the ferric reducing antioxidant power (FRAP) assays; and anticancer potential against two melanoma cancer cell lines (MM418C1 and D24) were analysed to determine the best extraction solvents. Results: Water was best for extracting trypsin inhibitors (581.4 ± 18.5 mg trypsin/mg) and reducing the viability of MM418C1 and D24 melanoma cells (75.5 ± 1.3 and 66.9 ± 2.2%, respectively); the anticancer potential against the MM418C1 cells was highly correlated with trypsin inhibitors (r = 0.92, p < 0.05), but there was no correlation between anticancer potential and antioxidant activity. The water saturated butanol had the highest saponins (71.8 ± 4.31 mg aescin equivalents/g), phenolic compounds (20.4 ± 0.86 mg gallic acid equivalents/g), and antioxidant activity, but these measures were not related to anticancer potential. Conclusions: Water yielded a Gac seed extract, rich in trypsin inhibitors, which had high anticancer potential against two melanoma cell lines.

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Authors+Show Affiliations

Le AV
School of Environmental and Life Sciences, University of Newcastle, Ourimbah, NSW 2258, Australia. vananh.le@uon.edu.au. Faculty of Bio-Food Technology and Environment, University of Technology (HUTECH), Ho Chi Minh City 700000, Vietnam. vananh.le@uon.edu.au.
Huynh TT
School of Science, RMIT University, Bundoora, VIC 3083, Australia. tien.huynh@rmit.edu.au.
Parks SE
School of Environmental and Life Sciences, University of Newcastle, Ourimbah, NSW 2258, Australia. sophie.parks@dpi.nsw.gov.au. Central Coast Primary Industries Centre, NSW Department of Primary Industries, Ourimbah, NSW 2258, Australia. sophie.parks@dpi.nsw.gov.au.
Nguyen MH
School of Environmental and Life Sciences, University of Newcastle, Ourimbah, NSW 2258, Australia. Minh.Nguyen@newcastle.edu.au. School of Science and Health, Western Sydney University, Penrith, NSW 2751, Australia. Minh.Nguyen@newcastle.edu.au.
Roach PD
School of Environmental and Life Sciences, University of Newcastle, Ourimbah, NSW 2258, Australia. Paul.Roach@newcastle.edu.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30231502

Citation

Le, Anh V., et al. "Bioactive Composition, Antioxidant Activity, and Anticancer Potential of Freeze-Dried Extracts From Defatted Gac (Momordica Cochinchinensis Spreng) Seeds." Medicines (Basel, Switzerland), vol. 5, no. 3, 2018.
Le AV, Huynh TT, Parks SE, et al. Bioactive Composition, Antioxidant Activity, and Anticancer Potential of Freeze-Dried Extracts from Defatted Gac (Momordica cochinchinensis Spreng) Seeds. Medicines (Basel). 2018;5(3).
Le, A. V., Huynh, T. T., Parks, S. E., Nguyen, M. H., & Roach, P. D. (2018). Bioactive Composition, Antioxidant Activity, and Anticancer Potential of Freeze-Dried Extracts from Defatted Gac (Momordica cochinchinensis Spreng) Seeds. Medicines (Basel, Switzerland), 5(3). https://doi.org/10.3390/medicines5030104
Le AV, et al. Bioactive Composition, Antioxidant Activity, and Anticancer Potential of Freeze-Dried Extracts From Defatted Gac (Momordica Cochinchinensis Spreng) Seeds. Medicines (Basel). 2018 Sep 18;5(3) PubMed PMID: 30231502.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioactive Composition, Antioxidant Activity, and Anticancer Potential of Freeze-Dried Extracts from Defatted Gac (Momordica cochinchinensis Spreng) Seeds. AU - Le,Anh V, AU - Huynh,Tien T, AU - Parks,Sophie E, AU - Nguyen,Minh H, AU - Roach,Paul D, Y1 - 2018/09/18/ PY - 2018/09/06/received PY - 2018/09/14/revised PY - 2018/09/15/accepted PY - 2018/9/21/entrez PY - 2018/9/21/pubmed PY - 2018/9/21/medline KW - Gac KW - Momordica cochinchinensis KW - anticancer KW - antioxidant KW - extraction KW - freeze dried extract KW - phenolics KW - saponins KW - seeds KW - trypsin inhibitors JF - Medicines (Basel, Switzerland) JO - Medicines (Basel) VL - 5 IS - 3 N2 - Background: Gac (Momordica cochinchinensis Spreng) seeds have long been used in traditional medicine as a remedy for numerous conditions due to a range of bioactive compounds. This study investigated the solvent extraction of compounds that could be responsible for antioxidant activity and anticancer potential. Methods: Defatted Gac seed kernel powder was extracted with different solvents: 100% water, 50% methanol:water, 70% ethanol:water, water saturated butanol, 100% methanol, and 100% ethanol. Trypsin inhibitors, saponins, phenolics, and antioxidant activity using the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the ferric reducing antioxidant power (FRAP) assays; and anticancer potential against two melanoma cancer cell lines (MM418C1 and D24) were analysed to determine the best extraction solvents. Results: Water was best for extracting trypsin inhibitors (581.4 ± 18.5 mg trypsin/mg) and reducing the viability of MM418C1 and D24 melanoma cells (75.5 ± 1.3 and 66.9 ± 2.2%, respectively); the anticancer potential against the MM418C1 cells was highly correlated with trypsin inhibitors (r = 0.92, p < 0.05), but there was no correlation between anticancer potential and antioxidant activity. The water saturated butanol had the highest saponins (71.8 ± 4.31 mg aescin equivalents/g), phenolic compounds (20.4 ± 0.86 mg gallic acid equivalents/g), and antioxidant activity, but these measures were not related to anticancer potential. Conclusions: Water yielded a Gac seed extract, rich in trypsin inhibitors, which had high anticancer potential against two melanoma cell lines. SN - 2305-6320 UR - https://www.unboundmedicine.com/medline/citation/30231502/Bioactive_Composition_Antioxidant_Activity_and_Anticancer_Potential_of_Freeze_Dried_Extracts_from_Defatted_Gac__Momordica_cochinchinensis_Spreng__Seeds_ DB - PRIME DP - Unbound Medicine ER -