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[Scalp-acupuncture Improves Neurological Function by Regulating Expression of NF-κB p 65 mRNA, IκB mRNA and IL-1 β and TNF-α in Parahippocampal Gyrus of Cerebral Ischemia Rats].
Zhen Ci Yan Jiu 2018; 43(9):556-61ZC

Abstract

OBJECTIVE

To observe the effect of scalp-acupuncture intervention on the expression of parahippocampal factor-κB p 65 mRNA (NF-κB p 65 mRNA), IκB mRNA, interleukin-1 β (IL-1 β) and tumor necrosis factor-α (TNF-α) in rats with cerebral ischemia (CI), so as to investigate its molecular mechanisms underlying improving CI by reducing inflammatory response.

METHODS

A total of 64 SD rats were randomized into normal control, model, medication and scalp-acupuncture groups, with 16 rats in each group. The focal CI model was established by middle cerebral artery occlusion (MCAO). Intraperitoneal injection of Pyrrolidine Dithiocarbamate (100 mg•kg-1•d-1) was administrated for rats in the medication group, once a day for 7 days. For rats of the scalp-acupuncture group, the acupuncture needles were rapidly inserted into bilateral Dingnieqianxiexian (MS 6) and Dingniehouxiexian (MS 7), followed by twirling the needles at 200 cycles/min for 1 min, once again every 10 min during 30 min's needle retention. The treatment was conducted once a day for 7 days. The neurologic deficit score (0-5 points, impaired consciousness, death, etc.) and neurological function score (motor, sensory and sensory tests, 0-10 points) were assessed according to Longa's (1989) and Schäbitz's (2004) methods, respectively. The expression levels of NF-κB p 65 mRNA and IκB mRNA in the parahippocampus gyrus tissue were detected by fluorescence quantitative reverse transcription-PCR, and IL-1 β and TNF-α proteins in the parahippocampus gyrus tissue were detected by immunohistochemistry.

RESULTS

After modeling, the neurologic deficit and neurological function scores and the expression levels of NF-κB p 65 mRNA, IL-1 β and TNF-α in the parahip-pocampus were significantly increased in the model group than in the normal group (P<0.01), while the expression of IκB mRNA was considerably down-regulated (P<0.01). Following treatment intervention, the neurologic deficit and neurological function scores as well as NF-κB p 65 mRNA, and IL-1 β and TNF-α protein expression were significantly decreased in both scalp-acupuncture and medication groups compared with the model group (P<0.05, P<0.01), and the decreased expression of IκB mRNA was obviously increased (P<0.05)..

CONCLUSION

Scalp-acupuncture can improve neurologic function in cerebral ischemic rats, which is related with its effects in up-regulating the expression of IκB to inhibit the dissociation of NF-κB, then decreasing the expression of IL-1 β and TNF-α (reducing inflammatory response) in the parahippocampal gyrus tissue.

Authors+Show Affiliations

Department of Traditional Chinese Medicine, Second Hospital of Lanzhou University, Lanzhou 730030, China.School of Acupuncture-moxibustion and Massage, Gansu University of Traditional Chinese Medicine, Lanzhou 730000.Department of Traditional Chinese Medicine, Second Hospital of Lanzhou University, Lanzhou 730030, China.Department of Traditional Chinese Medicine, Second Hospital of Lanzhou University, Lanzhou 730030, China.Department of Neurology, Second Hospital of Lanzhou University, Lanzhou 730030.Department of Neurology, Second Hospital of Lanzhou University, Lanzhou 730030.School of Acupuncture-moxibustion and Massage, Gansu University of Traditional Chinese Medicine, Lanzhou 730000.Department of Traditional Chinese Medicine, Second Hospital of Lanzhou University, Lanzhou 730030, China.School of Acupuncture-moxibustion and Massage, Gansu University of Traditional Chinese Medicine, Lanzhou 730000.School of Acupuncture-moxibustion and Massage, Gansu University of Traditional Chinese Medicine, Lanzhou 730000.School of Acupuncture-moxibustion and Massage, Gansu University of Traditional Chinese Medicine, Lanzhou 730000.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

30232863

Citation

Wang, Jin-Hai, et al. "[Scalp-acupuncture Improves Neurological Function By Regulating Expression of NF-κB P 65 mRNA, IκB mRNA and IL-1 Β and TNF-α in Parahippocampal Gyrus of Cerebral Ischemia Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 43, no. 9, 2018, pp. 556-61.
Wang JH, Zhang TZ, Zhao M, et al. [Scalp-acupuncture Improves Neurological Function by Regulating Expression of NF-κB p 65 mRNA, IκB mRNA and IL-1 β and TNF-α in Parahippocampal Gyrus of Cerebral Ischemia Rats]. Zhen Ci Yan Jiu. 2018;43(9):556-61.
Wang, J. H., Zhang, T. Z., Zhao, M., Bao, Y. C., Yan, Q., Zhang, Z. C., ... Li, X. L. (2018). [Scalp-acupuncture Improves Neurological Function by Regulating Expression of NF-κB p 65 mRNA, IκB mRNA and IL-1 β and TNF-α in Parahippocampal Gyrus of Cerebral Ischemia Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 43(9), pp. 556-61. doi:10.13702/j.1000-0607.170532.
Wang JH, et al. [Scalp-acupuncture Improves Neurological Function By Regulating Expression of NF-κB P 65 mRNA, IκB mRNA and IL-1 Β and TNF-α in Parahippocampal Gyrus of Cerebral Ischemia Rats]. Zhen Ci Yan Jiu. 2018 Sep 25;43(9):556-61. PubMed PMID: 30232863.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Scalp-acupuncture Improves Neurological Function by Regulating Expression of NF-κB p 65 mRNA, IκB mRNA and IL-1 β and TNF-α in Parahippocampal Gyrus of Cerebral Ischemia Rats]. AU - Wang,Jin-Hai, AU - Zhang,Ting-Zhuo, AU - Zhao,Min, AU - Bao,Ying-Cun, AU - Yan,Qi, AU - Zhang,Zhen-Chang, AU - Du,Xiao-Zheng, AU - Jiang,Hua, AU - Tian,Liang, AU - Yuan,Bo, AU - Li,Xing-Lan, PY - 2018/9/21/entrez PY - 2018/9/21/pubmed PY - 2019/4/9/medline KW - Acute cerebral ischemia KW - Interleukin-1 β (IL-1 β) KW - IκB mRNA KW - NF-κB p 65 mRNA KW - Neurologic deficit KW - Parahippocampus gyrus KW - Scalp acupuncture KW - Tumor necrosis factor-α (TNF-α)β SP - 556 EP - 61 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 43 IS - 9 N2 - OBJECTIVE: To observe the effect of scalp-acupuncture intervention on the expression of parahippocampal factor-κB p 65 mRNA (NF-κB p 65 mRNA), IκB mRNA, interleukin-1 β (IL-1 β) and tumor necrosis factor-α (TNF-α) in rats with cerebral ischemia (CI), so as to investigate its molecular mechanisms underlying improving CI by reducing inflammatory response. METHODS: A total of 64 SD rats were randomized into normal control, model, medication and scalp-acupuncture groups, with 16 rats in each group. The focal CI model was established by middle cerebral artery occlusion (MCAO). Intraperitoneal injection of Pyrrolidine Dithiocarbamate (100 mg•kg-1•d-1) was administrated for rats in the medication group, once a day for 7 days. For rats of the scalp-acupuncture group, the acupuncture needles were rapidly inserted into bilateral Dingnieqianxiexian (MS 6) and Dingniehouxiexian (MS 7), followed by twirling the needles at 200 cycles/min for 1 min, once again every 10 min during 30 min's needle retention. The treatment was conducted once a day for 7 days. The neurologic deficit score (0-5 points, impaired consciousness, death, etc.) and neurological function score (motor, sensory and sensory tests, 0-10 points) were assessed according to Longa's (1989) and Schäbitz's (2004) methods, respectively. The expression levels of NF-κB p 65 mRNA and IκB mRNA in the parahippocampus gyrus tissue were detected by fluorescence quantitative reverse transcription-PCR, and IL-1 β and TNF-α proteins in the parahippocampus gyrus tissue were detected by immunohistochemistry. RESULTS: After modeling, the neurologic deficit and neurological function scores and the expression levels of NF-κB p 65 mRNA, IL-1 β and TNF-α in the parahip-pocampus were significantly increased in the model group than in the normal group (P<0.01), while the expression of IκB mRNA was considerably down-regulated (P<0.01). Following treatment intervention, the neurologic deficit and neurological function scores as well as NF-κB p 65 mRNA, and IL-1 β and TNF-α protein expression were significantly decreased in both scalp-acupuncture and medication groups compared with the model group (P<0.05, P<0.01), and the decreased expression of IκB mRNA was obviously increased (P<0.05).. CONCLUSION: Scalp-acupuncture can improve neurologic function in cerebral ischemic rats, which is related with its effects in up-regulating the expression of IκB to inhibit the dissociation of NF-κB, then decreasing the expression of IL-1 β and TNF-α (reducing inflammatory response) in the parahippocampal gyrus tissue. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/30232863/[Scalp_acupuncture_Improves_Neurological_Function_by_Regulating_Expression_of_NF_κB_p_65_mRNA_IκB_mRNA_and_IL_1_β_and_TNF_α_in_Parahippocampal_Gyrus_of_Cerebral_Ischemia_Rats]_ DB - PRIME DP - Unbound Medicine ER -