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Transport of trehalose in Salmonella typhimurium.
J Bacteriol. 1986 Dec; 168(3):1107-11.JB

Abstract

We have studied trehalose uptake in Salmonella typhimurium and the possible involvement of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in this process. Two transport systems could recognize and transport trehalose, the mannose PTS and the galactose permease. Uptake of trehalose via the latter system required that it be expressed constitutively (due to a galR or galC mutation). Introduction of a ptsM mutation, resulting in a defective IIMan/IIIMan system, in S. typhimurium strains that grew on trehalose abolished growth on trehalose. A ptsG mutation, eliminating IIGlc of the glucose PTS, had no effect. In contrast, a crr mutation that resulted in the absence of IIIGlc of the glucose PTS prevented growth on trehalose. The inability of crr and also cya mutants to grow on trehalose was due to lowered intracellular cyclic AMP synthesis, since addition of extracellular cyclic AMP restored growth. Subsequent trehalose metabolism could be via a trehalose phosphate hydrolase, if trehalose phosphate was formed via the PTS, or trehalase. Trehalose-grown cells contained trehalase activity, but we could not detect phosphoenolpyruvate-dependent phosphorylation of trehalose in toluenized cells.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3023298

Citation

Postma, P W., et al. "Transport of Trehalose in Salmonella Typhimurium." Journal of Bacteriology, vol. 168, no. 3, 1986, pp. 1107-11.
Postma PW, Keizer HG, Koolwijk P. Transport of trehalose in Salmonella typhimurium. J Bacteriol. 1986;168(3):1107-11.
Postma, P. W., Keizer, H. G., & Koolwijk, P. (1986). Transport of trehalose in Salmonella typhimurium. Journal of Bacteriology, 168(3), 1107-11.
Postma PW, Keizer HG, Koolwijk P. Transport of Trehalose in Salmonella Typhimurium. J Bacteriol. 1986;168(3):1107-11. PubMed PMID: 3023298.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transport of trehalose in Salmonella typhimurium. AU - Postma,P W, AU - Keizer,H G, AU - Koolwijk,P, PY - 1986/12/1/pubmed PY - 1986/12/1/medline PY - 1986/12/1/entrez SP - 1107 EP - 11 JF - Journal of bacteriology JO - J Bacteriol VL - 168 IS - 3 N2 - We have studied trehalose uptake in Salmonella typhimurium and the possible involvement of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in this process. Two transport systems could recognize and transport trehalose, the mannose PTS and the galactose permease. Uptake of trehalose via the latter system required that it be expressed constitutively (due to a galR or galC mutation). Introduction of a ptsM mutation, resulting in a defective IIMan/IIIMan system, in S. typhimurium strains that grew on trehalose abolished growth on trehalose. A ptsG mutation, eliminating IIGlc of the glucose PTS, had no effect. In contrast, a crr mutation that resulted in the absence of IIIGlc of the glucose PTS prevented growth on trehalose. The inability of crr and also cya mutants to grow on trehalose was due to lowered intracellular cyclic AMP synthesis, since addition of extracellular cyclic AMP restored growth. Subsequent trehalose metabolism could be via a trehalose phosphate hydrolase, if trehalose phosphate was formed via the PTS, or trehalase. Trehalose-grown cells contained trehalase activity, but we could not detect phosphoenolpyruvate-dependent phosphorylation of trehalose in toluenized cells. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/3023298/Transport_of_trehalose_in_Salmonella_typhimurium_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=3023298 DB - PRIME DP - Unbound Medicine ER -