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Low nitric oxide level is implicated in sickle cell disease and its complications in Ghana.

Abstract

Background

Nitric oxide (NO) plays a fundamental role in maintaining normal vasomotor tone. Recent clinical and experimental data suggest that NO may play a role in the pathogenesis and therapy of sickle cell disease (SCD). The aim of this study was to determine NO metabolites (NOx) in SCD patients at steady state and in vaso-occlusive crisis (VOC), as well as those with hemolytic clinical sub-phenotype that includes leg ulcers and priapism.

Methodology

This was a case-control cross-sectional study conducted on a total of 694 subjects including 148 comparison group HbAA, 208 HbSS SCD patients in steady state, 82 HbSC SCD patients in steady state, 156 HbSS SCD patients in VOC, 34 HbSC SCD patients in VOC, 34 HbSS SCD patients in post VOC, 21 HbSS SCD patients with leg ulcer and 11 HbSS SCD patients with priapism, with age ranging from 15 to 65 years. Laboratory diagnosis of SCD was done at the Sickle Cell Clinic of the Korle-Bu Teaching Hospital. Plasma nitric oxide metabolites were measured using Griess reagent system by ELISA method.

Results

Mean NOx of 59.66±0.75 µMol/L in the comparison group was significantly different from those in steady state (P=0.02). During VOC, there was a significant reduction in mean NOx levels to 6.08±0.81 µMol/L (P<0.001). Mean NOx levels were however, significantly higher (50.97±1.68 µMol/L) (P<0.001) in the immediate postcrisis period. The mean NOx levels in the leg ulcer (21.70±1.18 µMol/L) (P<0.001) and priapism (28.97±1.27 µMol/L) (P<0.001) patients were significantly low as compared to the SCD patients in the steady state and comparison group.

Conclusion

This study presents the first report on plasma NOx levels in SCD complication in Ghanaian SCD patients and confirms reduced plasma NOx levels in SCD patients in general.

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  • Authors+Show Affiliations

    ,

    Department of Physiology, School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, Accra, Ghana, antwiboasiako@chs.edu.gh.

    Comprehensive Sickle Cell Program Children's National Medical Center, Division of Hematology, School of Medicine and Health Sciences George Washington University, Washington, DC, USA.

    Source

    MeSH

    Adolescent
    Adult
    Aged
    Anemia, Sickle Cell
    Biomarkers
    Case-Control Studies
    Cross-Sectional Studies
    Down-Regulation
    Enzyme-Linked Immunosorbent Assay
    Female
    Ghana
    Hemolysis
    Humans
    Leg Ulcer
    Male
    Middle Aged
    Nitric Oxide
    Priapism
    Prognosis
    Risk Factors
    Young Adult

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    30233199

    Citation

    Antwi-Boasiako, Charles, and Andrew D. Campbell. "Low Nitric Oxide Level Is Implicated in Sickle Cell Disease and Its Complications in Ghana." Vascular Health and Risk Management, vol. 14, 2018, pp. 199-204.
    Antwi-Boasiako C, Campbell AD. Low nitric oxide level is implicated in sickle cell disease and its complications in Ghana. Vasc Health Risk Manag. 2018;14:199-204.
    Antwi-Boasiako, C., & Campbell, A. D. (2018). Low nitric oxide level is implicated in sickle cell disease and its complications in Ghana. Vascular Health and Risk Management, 14, pp. 199-204. doi:10.2147/VHRM.S163228.
    Antwi-Boasiako C, Campbell AD. Low Nitric Oxide Level Is Implicated in Sickle Cell Disease and Its Complications in Ghana. Vasc Health Risk Manag. 2018;14:199-204. PubMed PMID: 30233199.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Low nitric oxide level is implicated in sickle cell disease and its complications in Ghana. AU - Antwi-Boasiako,Charles, AU - Campbell,Andrew D, Y1 - 2018/09/06/ PY - 2018/9/21/entrez PY - 2018/9/21/pubmed PY - 2018/11/6/medline KW - ELISA KW - leg ulcer KW - nitric oxide metabolites KW - priapism KW - sickle cell disease KW - vaso-occlusive crisis SP - 199 EP - 204 JF - Vascular health and risk management JO - Vasc Health Risk Manag VL - 14 N2 - Background: Nitric oxide (NO) plays a fundamental role in maintaining normal vasomotor tone. Recent clinical and experimental data suggest that NO may play a role in the pathogenesis and therapy of sickle cell disease (SCD). The aim of this study was to determine NO metabolites (NOx) in SCD patients at steady state and in vaso-occlusive crisis (VOC), as well as those with hemolytic clinical sub-phenotype that includes leg ulcers and priapism. Methodology: This was a case-control cross-sectional study conducted on a total of 694 subjects including 148 comparison group HbAA, 208 HbSS SCD patients in steady state, 82 HbSC SCD patients in steady state, 156 HbSS SCD patients in VOC, 34 HbSC SCD patients in VOC, 34 HbSS SCD patients in post VOC, 21 HbSS SCD patients with leg ulcer and 11 HbSS SCD patients with priapism, with age ranging from 15 to 65 years. Laboratory diagnosis of SCD was done at the Sickle Cell Clinic of the Korle-Bu Teaching Hospital. Plasma nitric oxide metabolites were measured using Griess reagent system by ELISA method. Results: Mean NOx of 59.66±0.75 µMol/L in the comparison group was significantly different from those in steady state (P=0.02). During VOC, there was a significant reduction in mean NOx levels to 6.08±0.81 µMol/L (P<0.001). Mean NOx levels were however, significantly higher (50.97±1.68 µMol/L) (P<0.001) in the immediate postcrisis period. The mean NOx levels in the leg ulcer (21.70±1.18 µMol/L) (P<0.001) and priapism (28.97±1.27 µMol/L) (P<0.001) patients were significantly low as compared to the SCD patients in the steady state and comparison group. Conclusion: This study presents the first report on plasma NOx levels in SCD complication in Ghanaian SCD patients and confirms reduced plasma NOx levels in SCD patients in general. SN - 1178-2048 UR - https://www.unboundmedicine.com/medline/citation/30233199/Low_nitric_oxide_level_is_implicated_in_sickle_cell_disease_and_its_complications_in_Ghana_ L2 - https://dx.doi.org/10.2147/VHRM.S163228 DB - PRIME DP - Unbound Medicine ER -