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Physicochemical and functional properties of Cucurbita maxima pumpkin pectin and commercial citrus and apple pectins: A comparative evaluation.
PLoS One. 2018; 13(9):e0204261.Plos

Abstract

The physicochemical characteristics and functional properties of pumpkin (Cucurbita maxima D. var. Cabello de Ángel) pectin obtained by cavitation facilitated extraction from pumpkin pulp have been evaluated and compared with commercial citrus and apple pectins. C. maxima pectin had an Mw value of 90 kDa and a high degree (72%) of esterification. The cytoprotective and antioxidant effects of citrus, apple and pumpkin pectin samples with different concentrations were studied in vitro in cell lines HT-29 (human colon adenocarcinoma) and MDCK1 (canine kidney epithelium). All pectin samples exhibited cytoprotective effect in HT-29 and MDCK1 cells after incubation with toxic concentrations of cadmium and mercury for 4 h. Pumpkin pectin increased the proliferation of cadmium-treated MDCK1 cells by 210%. The studied pectins also inhibited oxidative stress induced by 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AAPH) in cell cultures, as determined by measuring the production of intracellular reactive species using dihydrochlorofluorescein diacetate (DCFH-DA). Pectin from pumpkin pomace had the highest (p < 0.05) protective effect against reactive oxygen species generation in MDCK1 cells induced by AAPH. Distinctive features of pumpkin pectin were highly branched RG-I regions, the presence of RG-II regions and the highest galacturonic acid content among the studied samples of pectins. This correlates with a considerable protective effect of C. maxima pectin against oxidative stress and cytotoxicity induced by heavy metal ions. Thus, C. maxima pectin can be considered as a source of new functional foods of agricultural origin.

Authors+Show Affiliations

A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.Federal State-Owned Unitary Enterprise «All-Russian Research Institute for Optical and Physical Measurements», Moscow, Russia.A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia. Russian National Research Center "Kurchatov Institute", Moscow, Russia.Science and Technological Park of the University of Girona, Girona, Spain.A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30235297

Citation

Torkova, Anna A., et al. "Physicochemical and Functional Properties of Cucurbita Maxima Pumpkin Pectin and Commercial Citrus and Apple Pectins: a Comparative Evaluation." PloS One, vol. 13, no. 9, 2018, pp. e0204261.
Torkova AA, Lisitskaya KV, Filimonov IS, et al. Physicochemical and functional properties of Cucurbita maxima pumpkin pectin and commercial citrus and apple pectins: A comparative evaluation. PLoS ONE. 2018;13(9):e0204261.
Torkova, A. A., Lisitskaya, K. V., Filimonov, I. S., Glazunova, O. A., Kachalova, G. S., Golubev, V. N., & Fedorova, T. V. (2018). Physicochemical and functional properties of Cucurbita maxima pumpkin pectin and commercial citrus and apple pectins: A comparative evaluation. PloS One, 13(9), e0204261. https://doi.org/10.1371/journal.pone.0204261
Torkova AA, et al. Physicochemical and Functional Properties of Cucurbita Maxima Pumpkin Pectin and Commercial Citrus and Apple Pectins: a Comparative Evaluation. PLoS ONE. 2018;13(9):e0204261. PubMed PMID: 30235297.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physicochemical and functional properties of Cucurbita maxima pumpkin pectin and commercial citrus and apple pectins: A comparative evaluation. AU - Torkova,Anna A, AU - Lisitskaya,Ksenia V, AU - Filimonov,Ivan S, AU - Glazunova,Olga A, AU - Kachalova,Galina S, AU - Golubev,Vladimir N, AU - Fedorova,Tatyana V, Y1 - 2018/09/20/ PY - 2018/05/03/received PY - 2018/09/04/accepted PY - 2018/9/21/entrez PY - 2018/9/21/pubmed PY - 2019/3/5/medline SP - e0204261 EP - e0204261 JF - PloS one JO - PLoS ONE VL - 13 IS - 9 N2 - The physicochemical characteristics and functional properties of pumpkin (Cucurbita maxima D. var. Cabello de Ángel) pectin obtained by cavitation facilitated extraction from pumpkin pulp have been evaluated and compared with commercial citrus and apple pectins. C. maxima pectin had an Mw value of 90 kDa and a high degree (72%) of esterification. The cytoprotective and antioxidant effects of citrus, apple and pumpkin pectin samples with different concentrations were studied in vitro in cell lines HT-29 (human colon adenocarcinoma) and MDCK1 (canine kidney epithelium). All pectin samples exhibited cytoprotective effect in HT-29 and MDCK1 cells after incubation with toxic concentrations of cadmium and mercury for 4 h. Pumpkin pectin increased the proliferation of cadmium-treated MDCK1 cells by 210%. The studied pectins also inhibited oxidative stress induced by 2,2'-azobis(2-methylpropionamidine) dihydrochloride (AAPH) in cell cultures, as determined by measuring the production of intracellular reactive species using dihydrochlorofluorescein diacetate (DCFH-DA). Pectin from pumpkin pomace had the highest (p < 0.05) protective effect against reactive oxygen species generation in MDCK1 cells induced by AAPH. Distinctive features of pumpkin pectin were highly branched RG-I regions, the presence of RG-II regions and the highest galacturonic acid content among the studied samples of pectins. This correlates with a considerable protective effect of C. maxima pectin against oxidative stress and cytotoxicity induced by heavy metal ions. Thus, C. maxima pectin can be considered as a source of new functional foods of agricultural origin. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/30235297/Physicochemical_and_functional_properties_of_Cucurbita_maxima_pumpkin_pectin_and_commercial_citrus_and_apple_pectins:_A_comparative_evaluation_ L2 - http://dx.plos.org/10.1371/journal.pone.0204261 DB - PRIME DP - Unbound Medicine ER -