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Single-step detection of norovirus tuning localized surface plasmon resonance-induced optical signal between gold nanoparticles and quantum dots.
Biosens Bioelectron. 2018 Dec 30; 122:16-24.BB

Abstract

A new method of label free sensing approach with superior selectivity and sensitivity towards virlabel-freeon is presented here, employing the localized surface plasmon resonance (LSPR) behavior of gold nanoparticles (AuNPs) and fluorescent CdSeTeS quantum dots (QDs). Inorganic quaternary alloyed CdSeTeS QDs were capped with L-cysteine via a ligand exchange reaction. Alternatively, citrate stabilized AuNPs were functionalized with 11-mercaptoundecanoic acid to generate carboxylic group on the gold surface. The carboxylic group on the AuNPs was subjected to bind covalently with the amine group of L-cysteine capped CdSeTeS QDs to form CdSeTeS QDs/AuNPs nanocomposites. The fluorescence of CdSeTeS QDs/AuNPs nanocomposite shows quenched spectrum of CdSeTeS QDs at 640 nm due to the close interaction with AuNPs. However, after successive addition of norovirus-like particles (NoV-LPs), steric hindrance-induced LSPR signal from the adjacent AuNPs triggered the fluorescence enhancement of QDs in proportion to the concentration of the target NoV-LPs. A linear range of 10-14 to 10-9 g mL-1 NoV-LPs with a detection limit of 12.1 × 10-15 g mL-1 was obtained. This method was further applied on clinically isolated norovirus detection, in the range of 102-105 copies mL-1 with a detection limit of 95.0 copies mL-1, which is 100-fold higher than commercial ELISA kit. The superiority of the proposed sensor over other conventional sensors is found in its ultrasensitive detectability at low virus concentration even in clinically isolated samples. This proposed detection method can pave an avenue for the development of high performance and robust sensing probes for detection of virus in biomedical applications.

Authors+Show Affiliations

Laboratory of Biotechnology, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: fahmida.nasrin.17@shizuoka.ac.jp.Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: ankan.dutta.chowdhury@shizuoka.ac.jp.Laboratory of Biotechnology, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: takemura.kenshin.16@shizuoka.ac.jp.Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: lee.jaewook@shizuoka.ac.jp.Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: o.adegoke@dundee.ac.uk.Organization for International Collaboration, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: deo.vipin.kumar@shizuoka.ac.jp.Department of Microbiology, Shizuoka Institute of Environment and Hygiene, 4-27-2, Kita-ando, Aoi-ku, Shizuoka 420-8637, Japan. Electronic address: fuyuki1_abe@pref.shizuoka.lg.jp.Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Higashi-ku, Handa-yama, Hamamatsu 431-3192, Japan. Electronic address: tesuzuki@hama-med.ac.jp.Laboratory of Biotechnology, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: park.enoch@shizuoka.ac.jp.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

30236804

Citation

Nasrin, Fahmida, et al. "Single-step Detection of Norovirus Tuning Localized Surface Plasmon Resonance-induced Optical Signal Between Gold Nanoparticles and Quantum Dots." Biosensors & Bioelectronics, vol. 122, 2018, pp. 16-24.
Nasrin F, Chowdhury AD, Takemura K, et al. Single-step detection of norovirus tuning localized surface plasmon resonance-induced optical signal between gold nanoparticles and quantum dots. Biosens Bioelectron. 2018;122:16-24.
Nasrin, F., Chowdhury, A. D., Takemura, K., Lee, J., Adegoke, O., Deo, V. K., Abe, F., Suzuki, T., & Park, E. Y. (2018). Single-step detection of norovirus tuning localized surface plasmon resonance-induced optical signal between gold nanoparticles and quantum dots. Biosensors & Bioelectronics, 122, 16-24. https://doi.org/10.1016/j.bios.2018.09.024
Nasrin F, et al. Single-step Detection of Norovirus Tuning Localized Surface Plasmon Resonance-induced Optical Signal Between Gold Nanoparticles and Quantum Dots. Biosens Bioelectron. 2018 Dec 30;122:16-24. PubMed PMID: 30236804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Single-step detection of norovirus tuning localized surface plasmon resonance-induced optical signal between gold nanoparticles and quantum dots. AU - Nasrin,Fahmida, AU - Chowdhury,Ankan Dutta, AU - Takemura,Kenshin, AU - Lee,Jaewook, AU - Adegoke,Oluwasesan, AU - Deo,Vipin Kumar, AU - Abe,Fuyuki, AU - Suzuki,Tetsuro, AU - Park,Enoch Y, Y1 - 2018/09/07/ PY - 2018/06/07/received PY - 2018/08/21/revised PY - 2018/09/06/accepted PY - 2018/9/22/pubmed PY - 2019/1/24/medline PY - 2018/9/22/entrez KW - Biosensor KW - CdSeTeS KW - Gold nanoparticle KW - Localized surface plasmon resonance KW - Norovirus detection KW - Quantum dots SP - 16 EP - 24 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 122 N2 - A new method of label free sensing approach with superior selectivity and sensitivity towards virlabel-freeon is presented here, employing the localized surface plasmon resonance (LSPR) behavior of gold nanoparticles (AuNPs) and fluorescent CdSeTeS quantum dots (QDs). Inorganic quaternary alloyed CdSeTeS QDs were capped with L-cysteine via a ligand exchange reaction. Alternatively, citrate stabilized AuNPs were functionalized with 11-mercaptoundecanoic acid to generate carboxylic group on the gold surface. The carboxylic group on the AuNPs was subjected to bind covalently with the amine group of L-cysteine capped CdSeTeS QDs to form CdSeTeS QDs/AuNPs nanocomposites. The fluorescence of CdSeTeS QDs/AuNPs nanocomposite shows quenched spectrum of CdSeTeS QDs at 640 nm due to the close interaction with AuNPs. However, after successive addition of norovirus-like particles (NoV-LPs), steric hindrance-induced LSPR signal from the adjacent AuNPs triggered the fluorescence enhancement of QDs in proportion to the concentration of the target NoV-LPs. A linear range of 10-14 to 10-9 g mL-1 NoV-LPs with a detection limit of 12.1 × 10-15 g mL-1 was obtained. This method was further applied on clinically isolated norovirus detection, in the range of 102-105 copies mL-1 with a detection limit of 95.0 copies mL-1, which is 100-fold higher than commercial ELISA kit. The superiority of the proposed sensor over other conventional sensors is found in its ultrasensitive detectability at low virus concentration even in clinically isolated samples. This proposed detection method can pave an avenue for the development of high performance and robust sensing probes for detection of virus in biomedical applications. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/30236804/Single_step_detection_of_norovirus_tuning_localized_surface_plasmon_resonance_induced_optical_signal_between_gold_nanoparticles_and_quantum_dots_ DB - PRIME DP - Unbound Medicine ER -