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Dietary Fatty Acids Sustain the Growth of the Human Gut Microbiota.
Appl Environ Microbiol 2018; 84(21)AE

Abstract

While a substantial amount of dietary fats escape absorption in the human small intestine and reach the colon, the ability of resident microbiota to utilize these dietary fats for growth has not been investigated in detail. In this study, we used an in vitro multivessel simulator system of the human colon to reveal that the human gut microbiota is able to utilize typically consumed dietary fatty acids to sustain growth. Gut microbiota adapted quickly to a macronutrient switch from a balanced Western diet-type medium to its variant lacking carbohydrates and proteins. We defined specific genera that increased in their abundances on the fats-only medium, including Alistipes, Bilophila, and several genera of the class Gammaproteobacteria In contrast, the abundances of well-known glycan and protein degraders, including Bacteroides, Clostridium, and Roseburia spp., were reduced under such conditions. The predicted prevalences of microbial genes coding for fatty acid degradation enzymes and anaerobic respiratory reductases were significantly increased in the fats-only environment, whereas the abundance of glycan degradation genes was diminished. These changes also resulted in lower microbial production of short-chain fatty acids and antioxidants. Our findings provide justification for the previously observed alterations in gut microbiota observed in human and animal studies of high-fat diets.IMPORTANCE Increased intake of fats in many developed countries has raised awareness of potentially harmful and beneficial effects of high fat consumption on human health. Some dietary fats escape digestion in the small intestine and reach the colon where they can be metabolized by gut microbiota. We show that human gut microbes are able to maintain a complex community when supplied with dietary fatty acids as the only nutrient and carbon sources. Such fatty acid-based growth leads to lower production of short-chain fatty acids and antioxidants by community members, which potentially have negative health consequences on the host.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio, USA.Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio, USA.Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio, USA.Department of Nutrition and Food Science, School of Pharmacy, University of Granada, Granada, Spain.Department of Nutrition and Food Science, School of Pharmacy, University of Granada, Granada, Spain.Department of Biochemistry and Molecular Biology, Boonshoft School of Medicine, Wright State University, Dayton, Ohio, USA oleg.paliy@wright.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

30242004

Citation

Agans, Richard, et al. "Dietary Fatty Acids Sustain the Growth of the Human Gut Microbiota." Applied and Environmental Microbiology, vol. 84, no. 21, 2018.
Agans R, Gordon A, Kramer DL, et al. Dietary Fatty Acids Sustain the Growth of the Human Gut Microbiota. Appl Environ Microbiol. 2018;84(21).
Agans, R., Gordon, A., Kramer, D. L., Perez-Burillo, S., Rufián-Henares, J. A., & Paliy, O. (2018). Dietary Fatty Acids Sustain the Growth of the Human Gut Microbiota. Applied and Environmental Microbiology, 84(21), doi:10.1128/AEM.01525-18.
Agans R, et al. Dietary Fatty Acids Sustain the Growth of the Human Gut Microbiota. Appl Environ Microbiol. 2018 11 1;84(21) PubMed PMID: 30242004.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary Fatty Acids Sustain the Growth of the Human Gut Microbiota. AU - Agans,Richard, AU - Gordon,Alex, AU - Kramer,Denise Lynette, AU - Perez-Burillo,Sergio, AU - Rufián-Henares,José A, AU - Paliy,Oleg, Y1 - 2018/10/17/ PY - 2018/06/20/received PY - 2018/08/27/accepted PY - 2018/9/23/pubmed PY - 2018/9/23/medline PY - 2018/9/23/entrez KW - Western diet KW - dietary fats KW - microbial digestion KW - microbiota KW - nutrition JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 84 IS - 21 N2 - While a substantial amount of dietary fats escape absorption in the human small intestine and reach the colon, the ability of resident microbiota to utilize these dietary fats for growth has not been investigated in detail. In this study, we used an in vitro multivessel simulator system of the human colon to reveal that the human gut microbiota is able to utilize typically consumed dietary fatty acids to sustain growth. Gut microbiota adapted quickly to a macronutrient switch from a balanced Western diet-type medium to its variant lacking carbohydrates and proteins. We defined specific genera that increased in their abundances on the fats-only medium, including Alistipes, Bilophila, and several genera of the class Gammaproteobacteria In contrast, the abundances of well-known glycan and protein degraders, including Bacteroides, Clostridium, and Roseburia spp., were reduced under such conditions. The predicted prevalences of microbial genes coding for fatty acid degradation enzymes and anaerobic respiratory reductases were significantly increased in the fats-only environment, whereas the abundance of glycan degradation genes was diminished. These changes also resulted in lower microbial production of short-chain fatty acids and antioxidants. Our findings provide justification for the previously observed alterations in gut microbiota observed in human and animal studies of high-fat diets.IMPORTANCE Increased intake of fats in many developed countries has raised awareness of potentially harmful and beneficial effects of high fat consumption on human health. Some dietary fats escape digestion in the small intestine and reach the colon where they can be metabolized by gut microbiota. We show that human gut microbes are able to maintain a complex community when supplied with dietary fatty acids as the only nutrient and carbon sources. Such fatty acid-based growth leads to lower production of short-chain fatty acids and antioxidants by community members, which potentially have negative health consequences on the host. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/30242004/Dietary_Fatty_Acids_Sustain_the_Growth_of_the_Human_Gut_Microbiota_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=30242004 DB - PRIME DP - Unbound Medicine ER -