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The lncRNA small nucleolar RNA host gene 5 regulates trophoblast cell proliferation, invasion, and migration via modulating miR-26a-5p/N-cadherin axis.
J Cell Biochem 2019; 120(3):3173-3184JC

Abstract

Pre-eclampsia (PE) is a pregnancy-specific disease characterized by the occurrence of hypertension and proteinuria after two weeks of gestation. Long noncoding RNAs (lncRNAs) are emerging as key regulators in PE development. This study aims to investigate the role of lncRNA, small nucleolar RNA host gene 5 (SNHG5), in the pathogenesis of PE. The expression of SNHG5 was significantly downregulated in placental tissues from patients with severe PE compared normal controls. Overexpression of SNHG5 promoted trophoblast (HTR-8/SVneo) cell proliferation, invasion, and migration, and flow cytometry results showed that SNHG5 overexpression inhibited apoptosis and caused a decrease of cell population at the G 0 /G 1 phase and an increase of cell population at the S phase, while knockdown of SNHG5 had the opposite effects. The interaction between SNHG5 and miR-26a-5p was predicted by bioinformatics analysis and confirmed by luciferase reporter assay and RNA immunoprecipitation, and miR-26a-5p was negatively regulated by SNHG5; miR-26a-5p expression was upregulated in PE placental tissues and was inversely correlated with SNHG5 expression. Furthermore, miR-26a-5p was predicted to target the 3' untranslated region of N-cadherin, which was confirmed by luciferase reporter assay, and miR-26a-5p overexpression suppressed N-cadherin expression in HTR-8/SVneo cells. N-cadherin mRNA expression was downregulated in PE placental tissues and was positively correlated with SNHG5 expression. Both overexpression of miR-26a-5p and knockdown of N-cadherin suppressed HTR-8/SVneo cell invasion and migration, and also attenuated the effects of SNHG5 on the cellular functions of HTR-8/SVneo cells. In conclusion, our study suggested that SNHG5 promotes trophoblast cell proliferation, invasion, and migration at least partly via regulating the miR-26a-5p/N-cadherin axis.

Authors+Show Affiliations

Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xi'an Medical University, Xi'an, China.Department of Obstetrics, Baoji Maternal and Children Health Hospital, Baoji, China.Department of Gynecology and Obstetrics, Tangdu Hospital, Medical University of the Air Force, Xi'an, China.Department of Gynecology and Obstetrics, Tangdu Hospital, Medical University of the Air Force, Xi'an, China.Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xi'an Medical University, Xi'an, China.Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xi'an Medical University, Xi'an, China.Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xi'an Medical University, Xi'an, China.Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30242892

Citation

Yang, Yang, et al. "The lncRNA Small Nucleolar RNA Host Gene 5 Regulates Trophoblast Cell Proliferation, Invasion, and Migration Via Modulating miR-26a-5p/N-cadherin Axis." Journal of Cellular Biochemistry, vol. 120, no. 3, 2019, pp. 3173-3184.
Yang Y, Xi L, Ma Y, et al. The lncRNA small nucleolar RNA host gene 5 regulates trophoblast cell proliferation, invasion, and migration via modulating miR-26a-5p/N-cadherin axis. J Cell Biochem. 2019;120(3):3173-3184.
Yang, Y., Xi, L., Ma, Y., Zhu, X., Chen, R., Luan, L., ... An, R. (2019). The lncRNA small nucleolar RNA host gene 5 regulates trophoblast cell proliferation, invasion, and migration via modulating miR-26a-5p/N-cadherin axis. Journal of Cellular Biochemistry, 120(3), pp. 3173-3184. doi:10.1002/jcb.27583.
Yang Y, et al. The lncRNA Small Nucleolar RNA Host Gene 5 Regulates Trophoblast Cell Proliferation, Invasion, and Migration Via Modulating miR-26a-5p/N-cadherin Axis. J Cell Biochem. 2019;120(3):3173-3184. PubMed PMID: 30242892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The lncRNA small nucleolar RNA host gene 5 regulates trophoblast cell proliferation, invasion, and migration via modulating miR-26a-5p/N-cadherin axis. AU - Yang,Yang, AU - Xi,Lan, AU - Ma,Yuan, AU - Zhu,Xiaoming, AU - Chen,Rui, AU - Luan,Lixia, AU - Yan,Jiajia, AU - An,Ruifang, Y1 - 2018/09/22/ PY - 2018/03/07/received PY - 2018/08/08/accepted PY - 2018/9/23/pubmed PY - 2018/9/23/medline PY - 2018/9/23/entrez KW - N-cadherin KW - invasion and migration KW - miR-26a-5p KW - pre-eclampsia (PE) KW - small nucleolar RNA host gene 5 (SNHG5) KW - trophoblast SP - 3173 EP - 3184 JF - Journal of cellular biochemistry JO - J. Cell. Biochem. VL - 120 IS - 3 N2 - Pre-eclampsia (PE) is a pregnancy-specific disease characterized by the occurrence of hypertension and proteinuria after two weeks of gestation. Long noncoding RNAs (lncRNAs) are emerging as key regulators in PE development. This study aims to investigate the role of lncRNA, small nucleolar RNA host gene 5 (SNHG5), in the pathogenesis of PE. The expression of SNHG5 was significantly downregulated in placental tissues from patients with severe PE compared normal controls. Overexpression of SNHG5 promoted trophoblast (HTR-8/SVneo) cell proliferation, invasion, and migration, and flow cytometry results showed that SNHG5 overexpression inhibited apoptosis and caused a decrease of cell population at the G 0 /G 1 phase and an increase of cell population at the S phase, while knockdown of SNHG5 had the opposite effects. The interaction between SNHG5 and miR-26a-5p was predicted by bioinformatics analysis and confirmed by luciferase reporter assay and RNA immunoprecipitation, and miR-26a-5p was negatively regulated by SNHG5; miR-26a-5p expression was upregulated in PE placental tissues and was inversely correlated with SNHG5 expression. Furthermore, miR-26a-5p was predicted to target the 3' untranslated region of N-cadherin, which was confirmed by luciferase reporter assay, and miR-26a-5p overexpression suppressed N-cadherin expression in HTR-8/SVneo cells. N-cadherin mRNA expression was downregulated in PE placental tissues and was positively correlated with SNHG5 expression. Both overexpression of miR-26a-5p and knockdown of N-cadherin suppressed HTR-8/SVneo cell invasion and migration, and also attenuated the effects of SNHG5 on the cellular functions of HTR-8/SVneo cells. In conclusion, our study suggested that SNHG5 promotes trophoblast cell proliferation, invasion, and migration at least partly via regulating the miR-26a-5p/N-cadherin axis. SN - 1097-4644 UR - https://www.unboundmedicine.com/medline/citation/30242892/The_lncRNA_small_nucleolar_RNA_host_gene_5_regulates_trophoblast_cell_proliferation_invasion_and_migration_via_modulating_miR_26a_5p/N_cadherin_axis_ L2 - https://doi.org/10.1002/jcb.27583 DB - PRIME DP - Unbound Medicine ER -