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miRNA Expression Profile of Saliva in Subjects of Yang Deficiency Constitution and Yin Deficiency Constitution.
Cell Physiol Biochem. 2018; 49(5):2088-2098.CP

Abstract

BACKGROUND/AIMS

Based on the theory of constitution in Traditional Chinese Medicine (TCM), the Chinese Han population has been classified into nine constitutions. Of these, Yang deficiency constitution mainly exhibit cold intolerance while Yin deficiency constitution mainly exhibit heat intolerance. Some studies have been carried out to explore the modern genetic and biological basis of such constitution classification, but more remains to be done. MicroRNA (miRNA) serves as post-transcriptional regulators of gene expression and may play a role in the classification process. Here, we examined miRNA expression profile of saliva to further improve the comprehensiveness of constitution classification.

METHODS

Saliva was collected from Chinese Han individuals with Yang deficiency, Yin deficiency and Balanced constitutions (n=5 each), and miRNA expression profile was determined using the Human miRNA OneArray®v7. Based on 1.5 Fold change, means log2|Ratio|≥0.585 and P-value< 0.05, differentially expressed miRNA was screened. Target genes were predicted using DIANA-TarBasev7.0 and analysis of KEGG pathway was carried out using DIANA-mirPathv.3.

RESULTS

We found that 81 and 98 differentially expressed miRNAs were screened in Yang deficiency and Yin deficiency constitution, respectively. Among them, 16 miRNAs were identical and the others were unique. In addition, the target genes that are regulated by the unique miRNAs were significantly enriched in 27 and 20 signaling pathways in Yang deficiency and Yin deficiency constitution, respectively. Thyroid hormone signaling pathway is present in both constitutions. These unique miRNAs that regulated target genes of thyroid hormone signaling pathway may be associated with cold intolerance or heat intolerance.

CONCLUSION

The results of our study show that Yang deficiency and Yin deficiency constitutions exhibit systematic differences in miRNA expression profile. Moreover, the distinct characteristics of TCM constitution may be explained, in part, by differentially expressed miRNAs.

Authors+Show Affiliations

School of traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China. Department of Nutrition, China-Japan Friendship Hospital, Beijing, China.Department of Integrated Traditional and Western Medicine, People's Hospital of Wuhai Inner Mongolia, Wuhai, China.School of traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.School of traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.Department of Nutrition, China-Japan Friendship Hospital, Beijing, China.School of traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.School of traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.School of traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.School of traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.School of traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.School of traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.School of traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.School of traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30248664

Citation

Chen, Yu, et al. "MiRNA Expression Profile of Saliva in Subjects of Yang Deficiency Constitution and Yin Deficiency Constitution." Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, vol. 49, no. 5, 2018, pp. 2088-2098.
Chen Y, Wu Y, Yao H, et al. MiRNA Expression Profile of Saliva in Subjects of Yang Deficiency Constitution and Yin Deficiency Constitution. Cell Physiol Biochem. 2018;49(5):2088-2098.
Chen, Y., Wu, Y., Yao, H., Luo, H., Lin, B., Zhang, X., Liang, X., Sun, R., Zhao, S., Li, Y., Zheng, Y., Li, L., & Wang, Q. (2018). MiRNA Expression Profile of Saliva in Subjects of Yang Deficiency Constitution and Yin Deficiency Constitution. Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology, 49(5), 2088-2098. https://doi.org/10.1159/000493769
Chen Y, et al. MiRNA Expression Profile of Saliva in Subjects of Yang Deficiency Constitution and Yin Deficiency Constitution. Cell Physiol Biochem. 2018;49(5):2088-2098. PubMed PMID: 30248664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - miRNA Expression Profile of Saliva in Subjects of Yang Deficiency Constitution and Yin Deficiency Constitution. AU - Chen,Yu, AU - Wu,Yanling, AU - Yao,Haiqiang, AU - Luo,Hui, AU - Lin,Bing, AU - Zhang,Xiuping, AU - Liang,Xue, AU - Sun,Ranran, AU - Zhao,Shipeng, AU - Li,Yingshuai, AU - Zheng,Yanfei, AU - Li,Lingru, AU - Wang,Qi, Y1 - 2018/09/24/ PY - 2018/01/09/received PY - 2018/09/17/accepted PY - 2018/9/25/pubmed PY - 2018/10/17/medline PY - 2018/9/25/entrez KW - KEGG KW - Saliva KW - Target genes KW - Tcm constitution KW - miRNA SP - 2088 EP - 2098 JF - Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology JO - Cell Physiol Biochem VL - 49 IS - 5 N2 - BACKGROUND/AIMS: Based on the theory of constitution in Traditional Chinese Medicine (TCM), the Chinese Han population has been classified into nine constitutions. Of these, Yang deficiency constitution mainly exhibit cold intolerance while Yin deficiency constitution mainly exhibit heat intolerance. Some studies have been carried out to explore the modern genetic and biological basis of such constitution classification, but more remains to be done. MicroRNA (miRNA) serves as post-transcriptional regulators of gene expression and may play a role in the classification process. Here, we examined miRNA expression profile of saliva to further improve the comprehensiveness of constitution classification. METHODS: Saliva was collected from Chinese Han individuals with Yang deficiency, Yin deficiency and Balanced constitutions (n=5 each), and miRNA expression profile was determined using the Human miRNA OneArray®v7. Based on 1.5 Fold change, means log2|Ratio|≥0.585 and P-value< 0.05, differentially expressed miRNA was screened. Target genes were predicted using DIANA-TarBasev7.0 and analysis of KEGG pathway was carried out using DIANA-mirPathv.3. RESULTS: We found that 81 and 98 differentially expressed miRNAs were screened in Yang deficiency and Yin deficiency constitution, respectively. Among them, 16 miRNAs were identical and the others were unique. In addition, the target genes that are regulated by the unique miRNAs were significantly enriched in 27 and 20 signaling pathways in Yang deficiency and Yin deficiency constitution, respectively. Thyroid hormone signaling pathway is present in both constitutions. These unique miRNAs that regulated target genes of thyroid hormone signaling pathway may be associated with cold intolerance or heat intolerance. CONCLUSION: The results of our study show that Yang deficiency and Yin deficiency constitutions exhibit systematic differences in miRNA expression profile. Moreover, the distinct characteristics of TCM constitution may be explained, in part, by differentially expressed miRNAs. SN - 1421-9778 UR - https://www.unboundmedicine.com/medline/citation/30248664/miRNA_Expression_Profile_of_Saliva_in_Subjects_of_Yang_Deficiency_Constitution_and_Yin_Deficiency_Constitution_ L2 - https://www.karger.com?DOI=10.1159/000493769 DB - PRIME DP - Unbound Medicine ER -