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[Chromatographic behavior of basic drugs on thioether-embedded benzenesulfonate silica stationary phases].
Se Pu 2018; 36(9):850-857SP

Abstract

Mixed-mode chromatography (MMC) has pronounced advantages in the separation and analysis of complex samples. Compared with single-mode chromatography, the solute retention in MMC is controlled by multiple interactions, and the retention mechanism is more complicated. In this work, two thioether-embedded benzenesulfonate silica single-ligand and mixed-ligand stationary phases were prepared by thiol-ene click chemistry. The retention mechanisms of four basic drugs were investigated under various mobile phase compositions (pH, ionic and solvent strength). The results showed that both stationary phases have the mixed retention mechanism of the reverse phase and ion exchange. By changing the salt concentration of the mobile phase, the relationship between the retention factors of basic drugs and the reciprocal of ionic strength were investigated. The results indicate that a three-interaction-form model, containing reversed-phase, pure ion-exchange, and hydrophobically assisted ion-exchange interaction, is more suitable for the mechanism study of MMC. The quantitative results demonstrate that ion-exchange interaction composed of pure ion-exchange and hydrophobically assisted ion-exchange interaction is dominated on two phases. The relative contribution of each mechanism was varied with the solute, mobile phase composition, and ligand type and ratio. In addition, the hydrophobically assisted ion-exchange interaction had a significant impact on the solute retention and separation selectivity. These fundamental studies on the MMC retention mechanism are of great theoretical significance for the novel stationary phase design and the optimization of complex sample separation.

Authors+Show Affiliations

School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

30251512

Citation

Wang, Xiaohuan, and Lei Chen. "[Chromatographic Behavior of Basic Drugs On Thioether-embedded Benzenesulfonate Silica Stationary Phases]." Se Pu = Chinese Journal of Chromatography, vol. 36, no. 9, 2018, pp. 850-857.
Wang X, Chen L. [Chromatographic behavior of basic drugs on thioether-embedded benzenesulfonate silica stationary phases]. Se Pu. 2018;36(9):850-857.
Wang, X., & Chen, L. (2018). [Chromatographic behavior of basic drugs on thioether-embedded benzenesulfonate silica stationary phases]. Se Pu = Chinese Journal of Chromatography, 36(9), pp. 850-857. doi:10.3724/SP.J.1123.2018.04005.
Wang X, Chen L. [Chromatographic Behavior of Basic Drugs On Thioether-embedded Benzenesulfonate Silica Stationary Phases]. Se Pu. 2018 Sep 8;36(9):850-857. PubMed PMID: 30251512.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Chromatographic behavior of basic drugs on thioether-embedded benzenesulfonate silica stationary phases]. AU - Wang,Xiaohuan, AU - Chen,Lei, PY - 2018/9/26/entrez PY - 2018/9/27/pubmed PY - 2018/10/30/medline KW - basic drugs KW - cation-exchange KW - mixed-mode chromatography (MMC) KW - retention mechanism KW - reversed-phase KW - thiol-ene click chemistry SP - 850 EP - 857 JF - Se pu = Chinese journal of chromatography JO - Se Pu VL - 36 IS - 9 N2 - Mixed-mode chromatography (MMC) has pronounced advantages in the separation and analysis of complex samples. Compared with single-mode chromatography, the solute retention in MMC is controlled by multiple interactions, and the retention mechanism is more complicated. In this work, two thioether-embedded benzenesulfonate silica single-ligand and mixed-ligand stationary phases were prepared by thiol-ene click chemistry. The retention mechanisms of four basic drugs were investigated under various mobile phase compositions (pH, ionic and solvent strength). The results showed that both stationary phases have the mixed retention mechanism of the reverse phase and ion exchange. By changing the salt concentration of the mobile phase, the relationship between the retention factors of basic drugs and the reciprocal of ionic strength were investigated. The results indicate that a three-interaction-form model, containing reversed-phase, pure ion-exchange, and hydrophobically assisted ion-exchange interaction, is more suitable for the mechanism study of MMC. The quantitative results demonstrate that ion-exchange interaction composed of pure ion-exchange and hydrophobically assisted ion-exchange interaction is dominated on two phases. The relative contribution of each mechanism was varied with the solute, mobile phase composition, and ligand type and ratio. In addition, the hydrophobically assisted ion-exchange interaction had a significant impact on the solute retention and separation selectivity. These fundamental studies on the MMC retention mechanism are of great theoretical significance for the novel stationary phase design and the optimization of complex sample separation. SN - 1000-8713 UR - https://www.unboundmedicine.com/medline/citation/30251512/[Chromatographic_behavior_of_basic_drugs_on_thioether_embedded_benzenesulfonate_silica_stationary_phases]_ L2 - https://medlineplus.gov/medicines.html DB - PRIME DP - Unbound Medicine ER -