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Embryonic macrophages and microglia ablation alter the development of dorsal root ganglion sensory neurons in mouse embryos.
Glia. 2018 11; 66(11):2470-2486.GLIA

Abstract

Microglia are known to regulate several aspects of the development of the central nervous system. When microglia colonize the spinal cord, from E11.5 in the mouse embryo, they interact with growing central axons of dorsal root ganglion sensory neurons (SNs), which suggests that they may have some functions in SN development. To address this issue, we analyzed the effects of embryonic macrophage ablation on the early development of SNs using mouse embryo lacking embryonic macrophages (PU.1 knock-out mice) and immune cell ablation. We discovered that, in addition to microglia, embryonic macrophages contact tropomyosin receptor kinase (Trk) C+ SN, TrkB+ SN, and TrkA+ SN peripheral neurites from E11.5. Deprivation of immune cells resulted in an initial reduction of TrkC+ SN and TrkB+ SN populations at E11.5 that was unlikely to be related to an alteration in their developmental cell death (DCD), followed by a transitory increase in their number at E12.5. It also resulted in a reduction of TrkA+ SN number during the developmental period analyzed (E11.5-E15.5), although we did not observe any change in their DCD. Proliferation of cells negative for brain fatty acid-binding protein (BFABP-), which likely correspond to neuronal progenitors, was increased at E11.5, while their proliferation was decreased at E12.5, which could partly explain the alterations of SN subtype production observed from E11.5. In addition, we observed alterations in the proliferation of glial cell progenitors (BFABP+ cells) in the absence of embryonic macrophages. Our data indicate that embryonic macrophages and microglia ablation alter the development of SNs.

Authors+Show Affiliations

Sorbonne Université, UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris Seine, Institut de Biologie Paris Seine (NPS, IBPS), Paris, France. Neurophysiology and pharmacology laboratory, Federal University of Pernambuco, Pernambuco, Brazil.Sorbonne Université, UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris Seine, Institut de Biologie Paris Seine (NPS, IBPS), Paris, France. Neurophysiology and pharmacology laboratory, Federal University of Pernambuco, Pernambuco, Brazil.Sorbonne Université, UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris Seine, Institut de Biologie Paris Seine (NPS, IBPS), Paris, France.Singapore Immunology Network (SIgN), Agency for Science, Technology and Research, Singapore, Singapore.Singapore Immunology Network (SIgN), Agency for Science, Technology and Research, Singapore, Singapore.Neurophysiology and pharmacology laboratory, Federal University of Pernambuco, Pernambuco, Brazil.Sorbonne Université, UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris Seine, Institut de Biologie Paris Seine (NPS, IBPS), Paris, France.Sorbonne Université, UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris Seine, Institut de Biologie Paris Seine (NPS, IBPS), Paris, France. Université d'Angers, Angers, France.Sorbonne Université, UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris Seine, Institut de Biologie Paris Seine (NPS, IBPS), Paris, France.Sorbonne Université, UPMC Univ Paris 06, INSERM, CNRS, Neurosciences Paris Seine, Institut de Biologie Paris Seine (NPS, IBPS), Paris, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30252950

Citation

Angelim, Monara Kaélle Sérvulo Cruz, et al. "Embryonic Macrophages and Microglia Ablation Alter the Development of Dorsal Root Ganglion Sensory Neurons in Mouse Embryos." Glia, vol. 66, no. 11, 2018, pp. 2470-2486.
Angelim MKSC, Maia LMSS, Mouffle C, et al. Embryonic macrophages and microglia ablation alter the development of dorsal root ganglion sensory neurons in mouse embryos. Glia. 2018;66(11):2470-2486.
Angelim, M. K. S. C., Maia, L. M. S. S., Mouffle, C., Ginhoux, F., Low, D., Amancio-Dos-Santos, A., Makhoul, J., Le Corronc, H., Mangin, J. M., & Legendre, P. (2018). Embryonic macrophages and microglia ablation alter the development of dorsal root ganglion sensory neurons in mouse embryos. Glia, 66(11), 2470-2486. https://doi.org/10.1002/glia.23499
Angelim MKSC, et al. Embryonic Macrophages and Microglia Ablation Alter the Development of Dorsal Root Ganglion Sensory Neurons in Mouse Embryos. Glia. 2018;66(11):2470-2486. PubMed PMID: 30252950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Embryonic macrophages and microglia ablation alter the development of dorsal root ganglion sensory neurons in mouse embryos. AU - Angelim,Monara Kaélle Sérvulo Cruz, AU - Maia,Luciana Maria Silva de Seixas, AU - Mouffle,Christine, AU - Ginhoux,Florent, AU - Low,Donovan, AU - Amancio-Dos-Santos,Angela, AU - Makhoul,Jennifer, AU - Le Corronc,Hervé, AU - Mangin,Jean-Marie, AU - Legendre,Pascal, Y1 - 2018/09/25/ PY - 2018/02/01/received PY - 2018/06/29/revised PY - 2018/07/03/accepted PY - 2018/9/27/pubmed PY - 2019/3/27/medline PY - 2018/9/26/entrez KW - cell death KW - development KW - dorsal root ganglia KW - embryonic macrophage KW - microglia KW - neurogenesis SP - 2470 EP - 2486 JF - Glia JO - Glia VL - 66 IS - 11 N2 - Microglia are known to regulate several aspects of the development of the central nervous system. When microglia colonize the spinal cord, from E11.5 in the mouse embryo, they interact with growing central axons of dorsal root ganglion sensory neurons (SNs), which suggests that they may have some functions in SN development. To address this issue, we analyzed the effects of embryonic macrophage ablation on the early development of SNs using mouse embryo lacking embryonic macrophages (PU.1 knock-out mice) and immune cell ablation. We discovered that, in addition to microglia, embryonic macrophages contact tropomyosin receptor kinase (Trk) C+ SN, TrkB+ SN, and TrkA+ SN peripheral neurites from E11.5. Deprivation of immune cells resulted in an initial reduction of TrkC+ SN and TrkB+ SN populations at E11.5 that was unlikely to be related to an alteration in their developmental cell death (DCD), followed by a transitory increase in their number at E12.5. It also resulted in a reduction of TrkA+ SN number during the developmental period analyzed (E11.5-E15.5), although we did not observe any change in their DCD. Proliferation of cells negative for brain fatty acid-binding protein (BFABP-), which likely correspond to neuronal progenitors, was increased at E11.5, while their proliferation was decreased at E12.5, which could partly explain the alterations of SN subtype production observed from E11.5. In addition, we observed alterations in the proliferation of glial cell progenitors (BFABP+ cells) in the absence of embryonic macrophages. Our data indicate that embryonic macrophages and microglia ablation alter the development of SNs. SN - 1098-1136 UR - https://www.unboundmedicine.com/medline/citation/30252950/Embryonic_macrophages_and_microglia_ablation_alter_the_development_of_dorsal_root_ganglion_sensory_neurons_in_mouse_embryos_ L2 - https://doi.org/10.1002/glia.23499 DB - PRIME DP - Unbound Medicine ER -