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Lonicera japonica extends lifespan and healthspan in Caenorhabditis elegans.
Free Radic Biol Med. 2018 12; 129:310-322.FR

Abstract

Lonicera japonica (LJ) is widely used as the local medicine to improve body and prevent ills in China, but mechanisms of its healthy beneficial effects remain largely unclear. Here, we evaluated the anti-aging and healthspan promoting activities of 75% ethanol extract of LJ (LJ-E) in the animal model Caenorhabditis elegans. Our results showed that LJ-E (500 μg/mL) treatment enhanced the mean lifespan of worms by over 21.87% and significantly improved age-associated physiological functions in C. elegans. The 500 μg/mL concentration of LJ-E enhanced the survival rates under oxidative and thermal stresses, and decreased reactive oxygen species (ROS) levels and fat accumulation in the worms. Gene-specific mutant studies showed that LJ-E-mediated lifespan extension was dependent on mev-1, daf-2, daf-16, and hsf-1, but not eat-2 genes. LJ-E could upregulate stress-inducible genes, viz., hsp-16.2, sod-3 and mtl-1. Moreover, we found that the D1086.10 protein interacted with superoxide dismutase (SOD)-3 by functional protein association networks analysis according to RNA-sequencing results. It was confirmed that D1086.10 was needed to promote longevity, and positively regulated expression of sod-3 by using D1086.10 mutants. Furthermore, LJ-E significantly delayed amyloid β-protein induced paralysis in CL4176 strain. Given the important role of autophagy in aging and protein homeostasis, we observed that LJ-E could remarkably increase the mRNA expression of autophagy gene bec-1 in CL4176 strain, and decrease expression of autophagy substrate p62 protein by more than 40.0% in BC12921 strain. Finally, we found that combination composed of three major compounds (54 μg/mL chlorogenic acid, 15 μg/mL 1,5-dicaffeoylquinic acid and 7.5 μg/mL 1,3-dicaffeoylquinic acid) of 500 μg/mL LJ-E could significantly delay paralysis in CL4176 worms caused by Aβ toxicity, comparable to that of LJ-E. Overall, our study may have important implications in using Lonicera japonica to promote healthy aging and have a potency to design therapeutics for age-related diseases.

Authors+Show Affiliations

Research Center for Translational Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.Research Center for Translational Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.Research Center for Translational Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.Research Center for Translational Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.Research Center for Translational Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.Research Center for Translational Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China. Electronic address: hbwang@tongji.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

30266681

Citation

Yang, Zhen-Zhou, et al. "Lonicera Japonica Extends Lifespan and Healthspan in Caenorhabditis Elegans." Free Radical Biology & Medicine, vol. 129, 2018, pp. 310-322.
Yang ZZ, Yu YT, Lin HR, et al. Lonicera japonica extends lifespan and healthspan in Caenorhabditis elegans. Free Radic Biol Med. 2018;129:310-322.
Yang, Z. Z., Yu, Y. T., Lin, H. R., Liao, D. C., Cui, X. H., & Wang, H. B. (2018). Lonicera japonica extends lifespan and healthspan in Caenorhabditis elegans. Free Radical Biology & Medicine, 129, 310-322. https://doi.org/10.1016/j.freeradbiomed.2018.09.035
Yang ZZ, et al. Lonicera Japonica Extends Lifespan and Healthspan in Caenorhabditis Elegans. Free Radic Biol Med. 2018;129:310-322. PubMed PMID: 30266681.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lonicera japonica extends lifespan and healthspan in Caenorhabditis elegans. AU - Yang,Zhen-Zhou, AU - Yu,Ying-Ting, AU - Lin,Hong-Ru, AU - Liao,De-Chun, AU - Cui,Xiang-Huan, AU - Wang,Hong-Bing, Y1 - 2018/09/25/ PY - 2018/08/13/received PY - 2018/09/07/revised PY - 2018/09/21/accepted PY - 2018/9/30/pubmed PY - 2019/9/29/medline PY - 2018/9/30/entrez KW - Caenorhabditis elegans KW - Lifespan KW - Lonicera japonica KW - RNA-sequencing SP - 310 EP - 322 JF - Free radical biology & medicine JO - Free Radic. Biol. Med. VL - 129 N2 - Lonicera japonica (LJ) is widely used as the local medicine to improve body and prevent ills in China, but mechanisms of its healthy beneficial effects remain largely unclear. Here, we evaluated the anti-aging and healthspan promoting activities of 75% ethanol extract of LJ (LJ-E) in the animal model Caenorhabditis elegans. Our results showed that LJ-E (500 μg/mL) treatment enhanced the mean lifespan of worms by over 21.87% and significantly improved age-associated physiological functions in C. elegans. The 500 μg/mL concentration of LJ-E enhanced the survival rates under oxidative and thermal stresses, and decreased reactive oxygen species (ROS) levels and fat accumulation in the worms. Gene-specific mutant studies showed that LJ-E-mediated lifespan extension was dependent on mev-1, daf-2, daf-16, and hsf-1, but not eat-2 genes. LJ-E could upregulate stress-inducible genes, viz., hsp-16.2, sod-3 and mtl-1. Moreover, we found that the D1086.10 protein interacted with superoxide dismutase (SOD)-3 by functional protein association networks analysis according to RNA-sequencing results. It was confirmed that D1086.10 was needed to promote longevity, and positively regulated expression of sod-3 by using D1086.10 mutants. Furthermore, LJ-E significantly delayed amyloid β-protein induced paralysis in CL4176 strain. Given the important role of autophagy in aging and protein homeostasis, we observed that LJ-E could remarkably increase the mRNA expression of autophagy gene bec-1 in CL4176 strain, and decrease expression of autophagy substrate p62 protein by more than 40.0% in BC12921 strain. Finally, we found that combination composed of three major compounds (54 μg/mL chlorogenic acid, 15 μg/mL 1,5-dicaffeoylquinic acid and 7.5 μg/mL 1,3-dicaffeoylquinic acid) of 500 μg/mL LJ-E could significantly delay paralysis in CL4176 worms caused by Aβ toxicity, comparable to that of LJ-E. Overall, our study may have important implications in using Lonicera japonica to promote healthy aging and have a potency to design therapeutics for age-related diseases. SN - 1873-4596 UR - https://www.unboundmedicine.com/medline/citation/30266681/Lonicera_japonica_extends_lifespan_and_healthspan_in_Caenorhabditis_elegans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-5849(18)31395-9 DB - PRIME DP - Unbound Medicine ER -