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Snake venom proteome and immuno-profiling of the hundred-pace viper, Deinagkistrodon acutus, in Taiwan.
Acta Trop. 2019 Jan; 189:137-144.AT

Abstract

Deinagkistrodon acutus, also known as the hundred-pace viper or Chinese moccasin, is a clinically significant venomous snake in Taiwan. To address the lack of knowledge on the venom proteome of D. acutus, the venom composition was studied by a bottom-up proteomic approach combining reverse phase high-performance liquid chromatography, SDS-PAGE, and LC-MS/MS analysis. The immunoreactivity and cross-reactivity of Taiwanese freeze-dried D. acutus antivenom (DA-AV) and hemorrhagic antivenom (FH-AV) were investigated, as well. The proteomic analysis revealed the presence of 29 distinct proteins from D. acutus venom belonging to 8 snake venom protein families. Snake venom metalloproteinase (SVMP, 46.86%), C-type lectin (CLEC, 37.59%), phospholipase A2 (PLA2, 7.33%) and snake venom serine protease (SVSP, 6.62%) were the most abundant proteins. In addition to DA-AV, FH-AV also showed a profile of broad immunorecognition toward the venom of D. acutus. Remarkably, both antivenoms specifically reacted with the HPLC fractions containing SVMPs, and the titer was 5-10 times higher than fractions of other components. This information helps us to deeply understand the pathophysiology of D. acutus envenomation and guide us to development of more effective antivenom for clinical treatment.

Authors+Show Affiliations

Department of Emergency Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.Department of Emergency Medicine, En Chu Kong Hospital, New Taipei City, Taiwan; Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu, Taiwan.Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu, Taiwan; Divison of Nephrology, Department of Internal Medicine, En-Chu-Kong Hospital, New Taipei City, Taiwan; Renal Care Joint Foundation, Taipei, Taiwan.Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.Department of Emergency Medicine, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taoyuan, Taiwan. Electronic address: master198012@gmail.com.Department of Emergency Medicine, En Chu Kong Hospital, New Taipei City, Taiwan; Department of Medical Laboratory Science and Biotechnology, Yuanpei University, Hsinchu, Taiwan. Electronic address: sogahsieh@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30268686

Citation

Chen, Po-Chuan, et al. "Snake Venom Proteome and Immuno-profiling of the Hundred-pace Viper, Deinagkistrodon Acutus, in Taiwan." Acta Tropica, vol. 189, 2019, pp. 137-144.
Chen PC, Huang MN, Chang JF, et al. Snake venom proteome and immuno-profiling of the hundred-pace viper, Deinagkistrodon acutus, in Taiwan. Acta Trop. 2019;189:137-144.
Chen, P. C., Huang, M. N., Chang, J. F., Liu, C. C., Chen, C. K., & Hsieh, C. H. (2019). Snake venom proteome and immuno-profiling of the hundred-pace viper, Deinagkistrodon acutus, in Taiwan. Acta Tropica, 189, 137-144. https://doi.org/10.1016/j.actatropica.2018.09.017
Chen PC, et al. Snake Venom Proteome and Immuno-profiling of the Hundred-pace Viper, Deinagkistrodon Acutus, in Taiwan. Acta Trop. 2019;189:137-144. PubMed PMID: 30268686.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Snake venom proteome and immuno-profiling of the hundred-pace viper, Deinagkistrodon acutus, in Taiwan. AU - Chen,Po-Chuan, AU - Huang,Ming-Nan, AU - Chang,Jia-Feng, AU - Liu,Chien-Chun, AU - Chen,Chun-Kuei, AU - Hsieh,Cheng-Hsien, Y1 - 2018/09/27/ PY - 2018/03/06/received PY - 2018/07/31/revised PY - 2018/09/20/accepted PY - 2018/10/1/pubmed PY - 2019/1/3/medline PY - 2018/10/1/entrez KW - Antivenoms KW - Deinagkistrodon acutus KW - Immunoprofile KW - Venom proteome SP - 137 EP - 144 JF - Acta tropica JO - Acta Trop VL - 189 N2 - Deinagkistrodon acutus, also known as the hundred-pace viper or Chinese moccasin, is a clinically significant venomous snake in Taiwan. To address the lack of knowledge on the venom proteome of D. acutus, the venom composition was studied by a bottom-up proteomic approach combining reverse phase high-performance liquid chromatography, SDS-PAGE, and LC-MS/MS analysis. The immunoreactivity and cross-reactivity of Taiwanese freeze-dried D. acutus antivenom (DA-AV) and hemorrhagic antivenom (FH-AV) were investigated, as well. The proteomic analysis revealed the presence of 29 distinct proteins from D. acutus venom belonging to 8 snake venom protein families. Snake venom metalloproteinase (SVMP, 46.86%), C-type lectin (CLEC, 37.59%), phospholipase A2 (PLA2, 7.33%) and snake venom serine protease (SVSP, 6.62%) were the most abundant proteins. In addition to DA-AV, FH-AV also showed a profile of broad immunorecognition toward the venom of D. acutus. Remarkably, both antivenoms specifically reacted with the HPLC fractions containing SVMPs, and the titer was 5-10 times higher than fractions of other components. This information helps us to deeply understand the pathophysiology of D. acutus envenomation and guide us to development of more effective antivenom for clinical treatment. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/30268686/Snake_venom_proteome_and_immuno_profiling_of_the_hundred_pace_viper_Deinagkistrodon_acutus_in_Taiwan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(18)30268-7 DB - PRIME DP - Unbound Medicine ER -