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Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis.
World J Microbiol Biotechnol. 2018 Sep 29; 34(10):153.WJ

Abstract

Genome editing using engineered nucleases has rapidly transformed from a niche technology to a mainstream method used in various host cells. Its widespread adoption has been largely developed by the emergence of the clustered regularly interspaced short palindromic repeats (CRISPR) system, which uses an easily customizable specificity RNA-guided DNA endonuclease, such as Cas9. Recently, CRISPR/Cas9 mediated genome engineering has been widely applied to model organisms, including Bacillus subtilis, enabling facile, rapid high-fidelity modification of endogenous native genes. Here, we reviewed the recent progress in B. subtilis gene editing using CRISPR/Cas9 based tools, and highlighted state-of-the-art strategies for design of CRISPR/Cas9 system. Finally, future perspectives on the use of CRISPR/Cas9 genome engineering for sequence-specific genome editing in B. subtilis are provided.

Authors+Show Affiliations

Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China. Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People's Republic of China. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China.Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China. Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People's Republic of China. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China.Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China. Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People's Republic of China. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China.Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China. zww@tju.edu.cn. Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People's Republic of China. zww@tju.edu.cn. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, People's Republic of China. zww@tju.edu.cn.

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

30269229

Citation

Hong, Kun-Qiang, et al. "Recent Advances in CRISPR/Cas9 Mediated Genome Editing in Bacillus Subtilis." World Journal of Microbiology & Biotechnology, vol. 34, no. 10, 2018, p. 153.
Hong KQ, Liu DY, Chen T, et al. Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis. World J Microbiol Biotechnol. 2018;34(10):153.
Hong, K. Q., Liu, D. Y., Chen, T., & Wang, Z. W. (2018). Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis. World Journal of Microbiology & Biotechnology, 34(10), 153. https://doi.org/10.1007/s11274-018-2537-1
Hong KQ, et al. Recent Advances in CRISPR/Cas9 Mediated Genome Editing in Bacillus Subtilis. World J Microbiol Biotechnol. 2018 Sep 29;34(10):153. PubMed PMID: 30269229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis. AU - Hong,Kun-Qiang, AU - Liu,Ding-Yu, AU - Chen,Tao, AU - Wang,Zhi-Wen, Y1 - 2018/09/29/ PY - 2018/07/29/received PY - 2018/09/20/accepted PY - 2018/10/1/entrez PY - 2018/10/1/pubmed PY - 2018/10/16/medline KW - Bacillus subtilis KW - CRISPR/Cas9 KW - Genome editing KW - High-throughout SP - 153 EP - 153 JF - World journal of microbiology & biotechnology JO - World J Microbiol Biotechnol VL - 34 IS - 10 N2 - Genome editing using engineered nucleases has rapidly transformed from a niche technology to a mainstream method used in various host cells. Its widespread adoption has been largely developed by the emergence of the clustered regularly interspaced short palindromic repeats (CRISPR) system, which uses an easily customizable specificity RNA-guided DNA endonuclease, such as Cas9. Recently, CRISPR/Cas9 mediated genome engineering has been widely applied to model organisms, including Bacillus subtilis, enabling facile, rapid high-fidelity modification of endogenous native genes. Here, we reviewed the recent progress in B. subtilis gene editing using CRISPR/Cas9 based tools, and highlighted state-of-the-art strategies for design of CRISPR/Cas9 system. Finally, future perspectives on the use of CRISPR/Cas9 genome engineering for sequence-specific genome editing in B. subtilis are provided. SN - 1573-0972 UR - https://www.unboundmedicine.com/medline/citation/30269229/Recent_advances_in_CRISPR/Cas9_mediated_genome_editing_in_Bacillus_subtilis_ L2 - https://dx.doi.org/10.1007/s11274-018-2537-1 DB - PRIME DP - Unbound Medicine ER -