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Factors affecting the likelihood of pregnancy and embryonic loss after transfer of cryopreserved in vitro produced equine embryos.
Equine Vet J 2019; 51(4):446-450EV

Abstract

BACKGROUND

In vitro embryo production (IVEP) is increasingly popular but data assessing the outcome of transferred embryos are scarce.

OBJECTIVES

To determine the likelihood of pregnancy and embryonic loss after transfer of frozen-thawed IVP embryos and identify factors influencing success.

STUDY DESIGN

Retrospective clinical study.

METHODS

Blastocysts (n = 261) were produced from immature oocytes of Warmblood mares (n = 116) by Intracytoplasmic Sperm Injection (ICSI) and in vitro culture, and cryopreserved. Thawed IVP embryos were transferred into recipient mares on day 4, 5 or 6 after ovulation. The influence of donor mare (age, reproductive history), recipient mare (age, reproductive status, management; in-house vs. outpatient, day post-ovulation), embryo (interval from ICSI to blastocyst formation) and management factors (season when ovum pickup was performed, year and method of transfer) on likelihood of pregnancy and embryonic loss was examined, and the developmental stage of the IVP embryo at the time of transfer was estimated.

RESULTS

The percentage of mares pregnant 7-10, 23 and 37 days after transfer was 56% (147/261), 49% (129/261), and 48% (124/261), respectively. Development of IVP embryos after transfer equated to day 5 or 6 in vivo embryos. With the exception of year of transfer, none of the factors had an impact on the likelihood of pregnancy or embryonic loss. Nevertheless, the likelihood of pregnancy tended to be lower for IVP embryos from infertile mares or when embryos were transferred into recipient mares on day 6 after ovulation rather than on day 4 or 5. Finally, the diameter of the embryonic vesicle 7 days post transfer was lower for pregnancies that were lost compared to those that were maintained.

MAIN LIMITATIONS

Small sample size in some of the donor and recipient mare categories.

CONCLUSIONS

Cryopreserved IVP embryos should be transferred into recipient mares on day 4 or 5 after ovulation and a slower rate of post transfer vesicle expansion indicates a higher risk of subsequent embryonic loss The Summary is available in Portuguese - see Supporting Information.

Authors+Show Affiliations

Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.Department of Farm Animal Health, Section of Epidemiology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.Laboratory of Reproductive Technologies, Avantea, Cremona, Italy. Fondazione Avantea, Cremona, Italy.Laboratory of Reproductive Technologies, Avantea, Cremona, Italy.Laboratory of Reproductive Technologies, Avantea, Cremona, Italy. Fondazione Avantea, Cremona, Italy.Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30269336

Citation

Claes, A, et al. "Factors Affecting the Likelihood of Pregnancy and Embryonic Loss After Transfer of Cryopreserved in Vitro Produced Equine Embryos." Equine Veterinary Journal, vol. 51, no. 4, 2019, pp. 446-450.
Claes A, Cuervo-Arango J, van den Broek J, et al. Factors affecting the likelihood of pregnancy and embryonic loss after transfer of cryopreserved in vitro produced equine embryos. Equine Vet J. 2019;51(4):446-450.
Claes, A., Cuervo-Arango, J., van den Broek, J., Galli, C., Colleoni, S., Lazzari, G., ... Stout, T. A. (2019). Factors affecting the likelihood of pregnancy and embryonic loss after transfer of cryopreserved in vitro produced equine embryos. Equine Veterinary Journal, 51(4), pp. 446-450. doi:10.1111/evj.13028.
Claes A, et al. Factors Affecting the Likelihood of Pregnancy and Embryonic Loss After Transfer of Cryopreserved in Vitro Produced Equine Embryos. Equine Vet J. 2019;51(4):446-450. PubMed PMID: 30269336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Factors affecting the likelihood of pregnancy and embryonic loss after transfer of cryopreserved in vitro produced equine embryos. AU - Claes,A, AU - Cuervo-Arango,J, AU - van den Broek,J, AU - Galli,C, AU - Colleoni,S, AU - Lazzari,G, AU - Deelen,C, AU - Beitsma,M, AU - Stout,T A, Y1 - 2018/10/24/ PY - 2018/02/15/received PY - 2018/08/22/revised PY - 2018/09/24/accepted PY - 2018/10/1/pubmed PY - 2019/11/13/medline PY - 2018/10/1/entrez KW - embryonic loss KW - horse KW - intracytoplasmic sperm injection KW - in vitro embryo production KW - pregnancy SP - 446 EP - 450 JF - Equine veterinary journal JO - Equine Vet. J. VL - 51 IS - 4 N2 - BACKGROUND: In vitro embryo production (IVEP) is increasingly popular but data assessing the outcome of transferred embryos are scarce. OBJECTIVES: To determine the likelihood of pregnancy and embryonic loss after transfer of frozen-thawed IVP embryos and identify factors influencing success. STUDY DESIGN: Retrospective clinical study. METHODS: Blastocysts (n = 261) were produced from immature oocytes of Warmblood mares (n = 116) by Intracytoplasmic Sperm Injection (ICSI) and in vitro culture, and cryopreserved. Thawed IVP embryos were transferred into recipient mares on day 4, 5 or 6 after ovulation. The influence of donor mare (age, reproductive history), recipient mare (age, reproductive status, management; in-house vs. outpatient, day post-ovulation), embryo (interval from ICSI to blastocyst formation) and management factors (season when ovum pickup was performed, year and method of transfer) on likelihood of pregnancy and embryonic loss was examined, and the developmental stage of the IVP embryo at the time of transfer was estimated. RESULTS: The percentage of mares pregnant 7-10, 23 and 37 days after transfer was 56% (147/261), 49% (129/261), and 48% (124/261), respectively. Development of IVP embryos after transfer equated to day 5 or 6 in vivo embryos. With the exception of year of transfer, none of the factors had an impact on the likelihood of pregnancy or embryonic loss. Nevertheless, the likelihood of pregnancy tended to be lower for IVP embryos from infertile mares or when embryos were transferred into recipient mares on day 6 after ovulation rather than on day 4 or 5. Finally, the diameter of the embryonic vesicle 7 days post transfer was lower for pregnancies that were lost compared to those that were maintained. MAIN LIMITATIONS: Small sample size in some of the donor and recipient mare categories. CONCLUSIONS: Cryopreserved IVP embryos should be transferred into recipient mares on day 4 or 5 after ovulation and a slower rate of post transfer vesicle expansion indicates a higher risk of subsequent embryonic loss The Summary is available in Portuguese - see Supporting Information. SN - 2042-3306 UR - https://www.unboundmedicine.com/medline/citation/30269336/Factors_affecting_the_likelihood_of_pregnancy_and_embryonic_loss_after_transfer_of_cryopreserved_in_vitro_produced_equine_embryos_ L2 - https://doi.org/10.1111/evj.13028 DB - PRIME DP - Unbound Medicine ER -