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Thymoquinone protects human retinal pigment epithelial cells against hydrogen peroxide induced oxidative stress and apoptosis.
J Cell Biochem. 2019 03; 120(3):4514-4522.JC

Abstract

Oxidative stress in retinal pigment epithelium (RPE) cells may contribute to the progression of age-related macular degeneration. Thymoquinone (TQ), an active component derived from Nigella sativa, possesses antioxidative effect. However, the role of TQ in RPE cells under oxidative stress condition remains unclear. The present study aimed to examine the protective effect of TQ against hydrogen peroxide (H2 O2)-induced oxidative stress in human RPE cells. Our results showed that TQ improved the cell viability and apoptosis in H2 O2 -induced ARPE cells. We also found that the levels of reactive oxygen species and malondialdehyde induced by H2 O2 were reduced after the pretreatment of TQ. In addition, the inhibitory effect of H2 O2 on the glutathione (GSH) level and superoxide dismutase activity was markedly attenuated by TQ pretreatment. Moreover, TQ enhanced the activation of Nrf2/heme oxygenase 1 (HO-1) signaling pathway in H2 O2 -induced ARPE cells. Knockdown of Nrf2 abolished the protective effect of TQ on H2 O2 -induced oxidative damage. These results suggested that TQ protected ARPE cells from H2 O2 -induced oxidative stress and apoptosis via the Nrf2/HO-1 signaling pathway.

Authors+Show Affiliations

Department of Ophthalmology, Huaihe Hospital, Henan University, Kaifeng, China.Department of Ophthalmology, Huaihe Hospital, Henan University, Kaifeng, China.Department of Ophthalmology, Huaihe Hospital, Henan University, Kaifeng, China.Department of Ophthalmology, Huaihe Hospital, Henan University, Kaifeng, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

30269355

Citation

Hu, Xin, et al. "Thymoquinone Protects Human Retinal Pigment Epithelial Cells Against Hydrogen Peroxide Induced Oxidative Stress and Apoptosis." Journal of Cellular Biochemistry, vol. 120, no. 3, 2019, pp. 4514-4522.
Hu X, Liang Y, Zhao B, et al. Thymoquinone protects human retinal pigment epithelial cells against hydrogen peroxide induced oxidative stress and apoptosis. J Cell Biochem. 2019;120(3):4514-4522.
Hu, X., Liang, Y., Zhao, B., & Wang, Y. (2019). Thymoquinone protects human retinal pigment epithelial cells against hydrogen peroxide induced oxidative stress and apoptosis. Journal of Cellular Biochemistry, 120(3), 4514-4522. https://doi.org/10.1002/jcb.27739
Hu X, et al. Thymoquinone Protects Human Retinal Pigment Epithelial Cells Against Hydrogen Peroxide Induced Oxidative Stress and Apoptosis. J Cell Biochem. 2019;120(3):4514-4522. PubMed PMID: 30269355.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thymoquinone protects human retinal pigment epithelial cells against hydrogen peroxide induced oxidative stress and apoptosis. AU - Hu,Xin, AU - Liang,Yuanyuan, AU - Zhao,Bo, AU - Wang,Yongyi, Y1 - 2018/09/30/ PY - 2018/06/04/received PY - 2018/09/30/accepted PY - 2018/10/1/pubmed PY - 2020/3/19/medline PY - 2018/10/1/entrez KW - Nrf2/heme oxygenase 1 (HO-1) signaling pathway KW - age-related macular degeneration (AMD) KW - apoptosis KW - oxidative stress KW - retinal pigment epithelium (RPE) cells KW - thymoquinone (TQ) SP - 4514 EP - 4522 JF - Journal of cellular biochemistry JO - J. Cell. Biochem. VL - 120 IS - 3 N2 - Oxidative stress in retinal pigment epithelium (RPE) cells may contribute to the progression of age-related macular degeneration. Thymoquinone (TQ), an active component derived from Nigella sativa, possesses antioxidative effect. However, the role of TQ in RPE cells under oxidative stress condition remains unclear. The present study aimed to examine the protective effect of TQ against hydrogen peroxide (H2 O2)-induced oxidative stress in human RPE cells. Our results showed that TQ improved the cell viability and apoptosis in H2 O2 -induced ARPE cells. We also found that the levels of reactive oxygen species and malondialdehyde induced by H2 O2 were reduced after the pretreatment of TQ. In addition, the inhibitory effect of H2 O2 on the glutathione (GSH) level and superoxide dismutase activity was markedly attenuated by TQ pretreatment. Moreover, TQ enhanced the activation of Nrf2/heme oxygenase 1 (HO-1) signaling pathway in H2 O2 -induced ARPE cells. Knockdown of Nrf2 abolished the protective effect of TQ on H2 O2 -induced oxidative damage. These results suggested that TQ protected ARPE cells from H2 O2 -induced oxidative stress and apoptosis via the Nrf2/HO-1 signaling pathway. SN - 1097-4644 UR - https://www.unboundmedicine.com/medline/citation/30269355/Thymoquinone_protects_human_retinal_pigment_epithelial_cells_against_hydrogen_peroxide_induced_oxidative_stress_and_apoptosis_ L2 - https://doi.org/10.1002/jcb.27739 DB - PRIME DP - Unbound Medicine ER -